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Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression.

Guo SB, Li Q, Duan ZJ, Wang QM, Zhou Q, Sun XY - Mol Med Rep (2014)

Bottom Line: As compared with the sham group, hepatic HO-1 mRNA and protein expression levels, serum levels of ALT, AST and TBIL, COHb in arterial blood, hydroxyproline and collagen type I content were all significantly increased in the cirrhotic group.As compared with the cirrhotic group, the octreotide-treated group exhibited significantly reduced hepatic HO-1 expression levels, serum levels of ALT, AST and TBIL, COHb in arterial blood and the extent of hepatic fibrosis, whereas the cobalt protoporphyrin group exhibited significantly increased hepatic HO-1 expression levels, as well as aggravated hepatic function and fibrosis (P<0.05).In conclusion, octreotide inhibited hepatic HO-1 overexpression in cirrhotic rats, reduced hepatic HO-1 expression levels to relieve liver injury and attenuated liver fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 0086-116011, P.R. China.

ABSTRACT
The aim of the present study was to investigate the effects of octreotide treatment on hepatic heme oxygenase-1 (HO-1) expression, together with the influence of altered hepatic HO-1 expression levels on hepatic function and fibrosis in bile duct-ligated rats. The rats were divided randomly into sham, cirrhotic, cobalt protoporphyrin and octreotide treatment groups. The expression levels of hepatic HO-1 mRNA were measured by reverse-transcription polymerase chain reaction, while the protein expression was determined by western blotting and immunohistochemical analysis. Hematoxylin and eosin, and Van Gieson's staining, along with determination of the hydroxyproline content in the liver, were performed to determine the degree of liver fibrosis. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and carboxyhemoglobin (COHb) in arterial blood, and the mean arterial pressure and portal vein pressure were also measured. As compared with the sham group, hepatic HO-1 mRNA and protein expression levels, serum levels of ALT, AST and TBIL, COHb in arterial blood, hydroxyproline and collagen type I content were all significantly increased in the cirrhotic group. As compared with the cirrhotic group, the octreotide-treated group exhibited significantly reduced hepatic HO-1 expression levels, serum levels of ALT, AST and TBIL, COHb in arterial blood and the extent of hepatic fibrosis, whereas the cobalt protoporphyrin group exhibited significantly increased hepatic HO-1 expression levels, as well as aggravated hepatic function and fibrosis (P<0.05). In conclusion, octreotide inhibited hepatic HO-1 overexpression in cirrhotic rats, reduced hepatic HO-1 expression levels to relieve liver injury and attenuated liver fibrosis.

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Hepatic heme oxygenase-1 (HO-1) protein expression levels, detected by western blot analysis. (A) Hepatic HO-1 protein expression levels were greater in the bile duct ligation (BDL) group than in the Sham group; the levels were significantly higher in the cobalt protoporphyrin (CoPP) group and lower in the octreotide group (Oct), compared with the cirrhotic group. (B) Quantitative data: The ratio of the corresponding hepatic HO-1 protein expression levels to those of β-actin. aP<0.01 vs. sham group; bP<0.05 vs. BDL group. kDa, kilodaltons.
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f4-mmr-11-01-0083: Hepatic heme oxygenase-1 (HO-1) protein expression levels, detected by western blot analysis. (A) Hepatic HO-1 protein expression levels were greater in the bile duct ligation (BDL) group than in the Sham group; the levels were significantly higher in the cobalt protoporphyrin (CoPP) group and lower in the octreotide group (Oct), compared with the cirrhotic group. (B) Quantitative data: The ratio of the corresponding hepatic HO-1 protein expression levels to those of β-actin. aP<0.01 vs. sham group; bP<0.05 vs. BDL group. kDa, kilodaltons.

Mentions: Western blot analysis revealed that the protein expression levels of HO-1 were significantly higher in the BDL group compared with those of the sham group (P<0.01; Fig. 4); in addition, HO-1 expression was increased in the CoPP group and decreased in the octreotide group compared with that of the BDL group.


Octreotide attenuates liver fibrosis by inhibiting hepatic heme oxygenase-1 expression.

Guo SB, Li Q, Duan ZJ, Wang QM, Zhou Q, Sun XY - Mol Med Rep (2014)

Hepatic heme oxygenase-1 (HO-1) protein expression levels, detected by western blot analysis. (A) Hepatic HO-1 protein expression levels were greater in the bile duct ligation (BDL) group than in the Sham group; the levels were significantly higher in the cobalt protoporphyrin (CoPP) group and lower in the octreotide group (Oct), compared with the cirrhotic group. (B) Quantitative data: The ratio of the corresponding hepatic HO-1 protein expression levels to those of β-actin. aP<0.01 vs. sham group; bP<0.05 vs. BDL group. kDa, kilodaltons.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4237075&req=5

f4-mmr-11-01-0083: Hepatic heme oxygenase-1 (HO-1) protein expression levels, detected by western blot analysis. (A) Hepatic HO-1 protein expression levels were greater in the bile duct ligation (BDL) group than in the Sham group; the levels were significantly higher in the cobalt protoporphyrin (CoPP) group and lower in the octreotide group (Oct), compared with the cirrhotic group. (B) Quantitative data: The ratio of the corresponding hepatic HO-1 protein expression levels to those of β-actin. aP<0.01 vs. sham group; bP<0.05 vs. BDL group. kDa, kilodaltons.
Mentions: Western blot analysis revealed that the protein expression levels of HO-1 were significantly higher in the BDL group compared with those of the sham group (P<0.01; Fig. 4); in addition, HO-1 expression was increased in the CoPP group and decreased in the octreotide group compared with that of the BDL group.

Bottom Line: As compared with the sham group, hepatic HO-1 mRNA and protein expression levels, serum levels of ALT, AST and TBIL, COHb in arterial blood, hydroxyproline and collagen type I content were all significantly increased in the cirrhotic group.As compared with the cirrhotic group, the octreotide-treated group exhibited significantly reduced hepatic HO-1 expression levels, serum levels of ALT, AST and TBIL, COHb in arterial blood and the extent of hepatic fibrosis, whereas the cobalt protoporphyrin group exhibited significantly increased hepatic HO-1 expression levels, as well as aggravated hepatic function and fibrosis (P<0.05).In conclusion, octreotide inhibited hepatic HO-1 overexpression in cirrhotic rats, reduced hepatic HO-1 expression levels to relieve liver injury and attenuated liver fibrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 0086-116011, P.R. China.

ABSTRACT
The aim of the present study was to investigate the effects of octreotide treatment on hepatic heme oxygenase-1 (HO-1) expression, together with the influence of altered hepatic HO-1 expression levels on hepatic function and fibrosis in bile duct-ligated rats. The rats were divided randomly into sham, cirrhotic, cobalt protoporphyrin and octreotide treatment groups. The expression levels of hepatic HO-1 mRNA were measured by reverse-transcription polymerase chain reaction, while the protein expression was determined by western blotting and immunohistochemical analysis. Hematoxylin and eosin, and Van Gieson's staining, along with determination of the hydroxyproline content in the liver, were performed to determine the degree of liver fibrosis. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and carboxyhemoglobin (COHb) in arterial blood, and the mean arterial pressure and portal vein pressure were also measured. As compared with the sham group, hepatic HO-1 mRNA and protein expression levels, serum levels of ALT, AST and TBIL, COHb in arterial blood, hydroxyproline and collagen type I content were all significantly increased in the cirrhotic group. As compared with the cirrhotic group, the octreotide-treated group exhibited significantly reduced hepatic HO-1 expression levels, serum levels of ALT, AST and TBIL, COHb in arterial blood and the extent of hepatic fibrosis, whereas the cobalt protoporphyrin group exhibited significantly increased hepatic HO-1 expression levels, as well as aggravated hepatic function and fibrosis (P<0.05). In conclusion, octreotide inhibited hepatic HO-1 overexpression in cirrhotic rats, reduced hepatic HO-1 expression levels to relieve liver injury and attenuated liver fibrosis.

Show MeSH
Related in: MedlinePlus