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Determination of sunset yellow and tartrazine in food samples by combining ionic liquid-based aqueous two-phase system with high performance liquid chromatography.

Sha O, Zhu X, Feng Y, Ma W - J Anal Methods Chem (2014)

Bottom Line: Calibration plots were linear in the range of 0.01-50.0 μg/mL for both Ta and SY.The limits of detection were 5.2 ng/mL for Ta and 6.9 ng/mL for SY.This method proves successful for the separation/analysis of tartrazine and sunset yellow in soft drink sample, candy sample, and instant powder drink and leads to consistent results as obtained from the Chinese national standard method.

View Article: PubMed Central - PubMed

Affiliation: Analysis and Test Centre of Jiangsu Marine Resources Development Research Institute, Lianyungang 222001, China ; College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China ; School of Chemistry and Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, China.

ABSTRACT
We proposed a simple and effective method, by coupling ionic liquid-based aqueous two-phase systems (IL-ATPSs) with high performance liquid chromatography (HPLC), for the analysis of determining tartrazine and sunset yellow in food samples. Under the optimized conditions, IL-ATPSs generated an extraction efficiency of 99% for both analytes, which could then be directly analyzed by HPLC without further treatment. Calibration plots were linear in the range of 0.01-50.0 μg/mL for both Ta and SY. The limits of detection were 5.2 ng/mL for Ta and 6.9 ng/mL for SY. This method proves successful for the separation/analysis of tartrazine and sunset yellow in soft drink sample, candy sample, and instant powder drink and leads to consistent results as obtained from the Chinese national standard method.

No MeSH data available.


Related in: MedlinePlus

Effects of K2HPO4 amount on extraction efficiency, partition coefficient, and phase ratio (mSY = 100 μg, mTa = 100 μg, VIL = 0.30 mL).
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fig3: Effects of K2HPO4 amount on extraction efficiency, partition coefficient, and phase ratio (mSY = 100 μg, mTa = 100 μg, VIL = 0.30 mL).

Mentions: With a fixed volume of [C4MIM]Br at 0.3 mL, [C4MIM]Br-K2HPO4 ATPS was formed when the amount of K2HPO4 was over 2.7 g in weight for Ta and 2.9 g in weight for SY, respectively. The influence of K2HPO4 concentration on the extraction efficiency, partition coefficient, and phase ratio was studied with 0.3 mL [C4MIM]Br added and the results obtained were shown in Figure 3. It revealed that the extraction efficiency obtained was above 95% with K2HPO4 of 3.00–3.40 g (Figure 3(a)). The partition coefficient of Ta and SY was increased to a maximum value when the amount of K2HPO4 was set at 3.0 g and 3.1 g, respectively, then decreased with the increased amount of K2HPO4. The phase ratio of Ta and SY was increased with the amount of K2HPO4 (Figure 3(b)). In order to achieve quantitative extraction and higher separation efficiency, 3.0 g of K2HPO4 was used in all following experiments for the simultaneous extraction of SY and Ta.


Determination of sunset yellow and tartrazine in food samples by combining ionic liquid-based aqueous two-phase system with high performance liquid chromatography.

Sha O, Zhu X, Feng Y, Ma W - J Anal Methods Chem (2014)

Effects of K2HPO4 amount on extraction efficiency, partition coefficient, and phase ratio (mSY = 100 μg, mTa = 100 μg, VIL = 0.30 mL).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4236886&req=5

fig3: Effects of K2HPO4 amount on extraction efficiency, partition coefficient, and phase ratio (mSY = 100 μg, mTa = 100 μg, VIL = 0.30 mL).
Mentions: With a fixed volume of [C4MIM]Br at 0.3 mL, [C4MIM]Br-K2HPO4 ATPS was formed when the amount of K2HPO4 was over 2.7 g in weight for Ta and 2.9 g in weight for SY, respectively. The influence of K2HPO4 concentration on the extraction efficiency, partition coefficient, and phase ratio was studied with 0.3 mL [C4MIM]Br added and the results obtained were shown in Figure 3. It revealed that the extraction efficiency obtained was above 95% with K2HPO4 of 3.00–3.40 g (Figure 3(a)). The partition coefficient of Ta and SY was increased to a maximum value when the amount of K2HPO4 was set at 3.0 g and 3.1 g, respectively, then decreased with the increased amount of K2HPO4. The phase ratio of Ta and SY was increased with the amount of K2HPO4 (Figure 3(b)). In order to achieve quantitative extraction and higher separation efficiency, 3.0 g of K2HPO4 was used in all following experiments for the simultaneous extraction of SY and Ta.

Bottom Line: Calibration plots were linear in the range of 0.01-50.0 μg/mL for both Ta and SY.The limits of detection were 5.2 ng/mL for Ta and 6.9 ng/mL for SY.This method proves successful for the separation/analysis of tartrazine and sunset yellow in soft drink sample, candy sample, and instant powder drink and leads to consistent results as obtained from the Chinese national standard method.

View Article: PubMed Central - PubMed

Affiliation: Analysis and Test Centre of Jiangsu Marine Resources Development Research Institute, Lianyungang 222001, China ; College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, China ; School of Chemistry and Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, China.

ABSTRACT
We proposed a simple and effective method, by coupling ionic liquid-based aqueous two-phase systems (IL-ATPSs) with high performance liquid chromatography (HPLC), for the analysis of determining tartrazine and sunset yellow in food samples. Under the optimized conditions, IL-ATPSs generated an extraction efficiency of 99% for both analytes, which could then be directly analyzed by HPLC without further treatment. Calibration plots were linear in the range of 0.01-50.0 μg/mL for both Ta and SY. The limits of detection were 5.2 ng/mL for Ta and 6.9 ng/mL for SY. This method proves successful for the separation/analysis of tartrazine and sunset yellow in soft drink sample, candy sample, and instant powder drink and leads to consistent results as obtained from the Chinese national standard method.

No MeSH data available.


Related in: MedlinePlus