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Karyotypic diversity in seven Amazonian anurans in the genus Hypsiboas (family Hylidae).

de Mattos TL, Coelho AC, Schneider CH, Telles DO, Menin M, Gross MC - BMC Genet. (2014)

Bottom Line: The data were compared with a previous phylogenetic proposal for these anurans.The results of this study reinforce the complexity previously observed within the genus Hypsiboas and in the different groups that compose this taxon.More studies are needed focusing on this group and covering larger sampling areas, especially in the Brazilian Amazon, to improve our understanding of this fascinating and complex group.

View Article: PubMed Central - HTML - PubMed

Affiliation: Instituto Nacional de Pesquisas da Amazônia, Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva, Av, André Araújo, 2936, 69080-971 Manaus, AM, Brazil. tldmattos@gmail.com.

ABSTRACT

Background: Hypsiboas species have been divided into seven groups using morphological and genetic characters, but for most of the species, there is no cytogenetic information available. A cytogenetic analysis using conventional staining, C-banding, silver staining, and fluorescence in situ hybridization (FISH) with telomeric sequence probes were used to investigate the karyotype of seven Amazon species of the genus Hypsiboas belonging to the following intrageneric groups: H. punctatus (H. cinerascens), H. semilineatus (H. boans, H. geographicus, and H. wavrini), and H. albopunctatus (H. lanciformis, H. multifasciatus, and H. raniceps). The aim was to differentiate between the karyotypes and use the chromosomal markers to distinguish between the Hypsiboas groups. The data were compared with a previous phylogenetic proposal for these anurans. In addition, H. lanciformis, H. boans, and H. wavrini are described here for the first time, and we characterize the diploid numbers for H. cinerascens, H. geographicus, H. multifasciatus, and H. raniceps.

Results: The diploid number for all of the species analyzed was 24, with the exception of Hypsiboas lanciformis, which had 2n = 22 chromosomes. The constitutive heterochromatin distribution, nucleolar organizer region locations, and interstitial telomeric sites differed between the species. A hypothesis that the heterochromatic patterns are evolving is proposed, with the divergence of the groups probably involving events such as an increase in the heterochromatin in the species of the H. semilineatus group. The FISH conducted with the telomeric probes detected sites in the terminal regions of all of the chromosomes of all species. Interstitial telomeric sites were detected in three species belonging to the H. semilineatus group: H. boans, H. geographicus, and H. wavrini.

Conclusion: The results of this study reinforce the complexity previously observed within the genus Hypsiboas and in the different groups that compose this taxon. More studies are needed focusing on this group and covering larger sampling areas, especially in the Brazilian Amazon, to improve our understanding of this fascinating and complex group.

Show MeSH
Telomeric hybridization. Hypsiboas boans (a), H. geographicus (b), and H. wavrini (c), which are in the H. semilineatus group, showed signs of hybridization based on the telomeric probe (red). The chromosomes were counterstained with DAPI.
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Figure 4: Telomeric hybridization. Hypsiboas boans (a), H. geographicus (b), and H. wavrini (c), which are in the H. semilineatus group, showed signs of hybridization based on the telomeric probe (red). The chromosomes were counterstained with DAPI.

Mentions: Combining telomeric probes with fluorescence in situ hybridization (FISH) detected sites in the terminal regions of all of the chromosomes of all species. Interstitial telomeric sites (ITSs) were detected in three of the species belonging to the H. semilineatus group: H. boans, H. geographicus, and H. wavrini. In H. boans and H. wavrini (Figures 4a and c, respectively), the ITSs were seen on the short arms of both homologs of pair 2 and on the long arms of both homologs of pair 5. The centromeric ITSs in H. geographicus were seen on both homologs of pairs 1 and 5; the ITSs on pair 1 correspond with the NOR sites in this species (Figure 4b). However, ITSs were not found in H. cinerascens, H. lanciformis, H. multifasciatus and H. raniceps.


Karyotypic diversity in seven Amazonian anurans in the genus Hypsiboas (family Hylidae).

de Mattos TL, Coelho AC, Schneider CH, Telles DO, Menin M, Gross MC - BMC Genet. (2014)

Telomeric hybridization. Hypsiboas boans (a), H. geographicus (b), and H. wavrini (c), which are in the H. semilineatus group, showed signs of hybridization based on the telomeric probe (red). The chromosomes were counterstained with DAPI.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4234493&req=5

Figure 4: Telomeric hybridization. Hypsiboas boans (a), H. geographicus (b), and H. wavrini (c), which are in the H. semilineatus group, showed signs of hybridization based on the telomeric probe (red). The chromosomes were counterstained with DAPI.
Mentions: Combining telomeric probes with fluorescence in situ hybridization (FISH) detected sites in the terminal regions of all of the chromosomes of all species. Interstitial telomeric sites (ITSs) were detected in three of the species belonging to the H. semilineatus group: H. boans, H. geographicus, and H. wavrini. In H. boans and H. wavrini (Figures 4a and c, respectively), the ITSs were seen on the short arms of both homologs of pair 2 and on the long arms of both homologs of pair 5. The centromeric ITSs in H. geographicus were seen on both homologs of pairs 1 and 5; the ITSs on pair 1 correspond with the NOR sites in this species (Figure 4b). However, ITSs were not found in H. cinerascens, H. lanciformis, H. multifasciatus and H. raniceps.

Bottom Line: The data were compared with a previous phylogenetic proposal for these anurans.The results of this study reinforce the complexity previously observed within the genus Hypsiboas and in the different groups that compose this taxon.More studies are needed focusing on this group and covering larger sampling areas, especially in the Brazilian Amazon, to improve our understanding of this fascinating and complex group.

View Article: PubMed Central - HTML - PubMed

Affiliation: Instituto Nacional de Pesquisas da Amazônia, Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva, Av, André Araújo, 2936, 69080-971 Manaus, AM, Brazil. tldmattos@gmail.com.

ABSTRACT

Background: Hypsiboas species have been divided into seven groups using morphological and genetic characters, but for most of the species, there is no cytogenetic information available. A cytogenetic analysis using conventional staining, C-banding, silver staining, and fluorescence in situ hybridization (FISH) with telomeric sequence probes were used to investigate the karyotype of seven Amazon species of the genus Hypsiboas belonging to the following intrageneric groups: H. punctatus (H. cinerascens), H. semilineatus (H. boans, H. geographicus, and H. wavrini), and H. albopunctatus (H. lanciformis, H. multifasciatus, and H. raniceps). The aim was to differentiate between the karyotypes and use the chromosomal markers to distinguish between the Hypsiboas groups. The data were compared with a previous phylogenetic proposal for these anurans. In addition, H. lanciformis, H. boans, and H. wavrini are described here for the first time, and we characterize the diploid numbers for H. cinerascens, H. geographicus, H. multifasciatus, and H. raniceps.

Results: The diploid number for all of the species analyzed was 24, with the exception of Hypsiboas lanciformis, which had 2n = 22 chromosomes. The constitutive heterochromatin distribution, nucleolar organizer region locations, and interstitial telomeric sites differed between the species. A hypothesis that the heterochromatic patterns are evolving is proposed, with the divergence of the groups probably involving events such as an increase in the heterochromatin in the species of the H. semilineatus group. The FISH conducted with the telomeric probes detected sites in the terminal regions of all of the chromosomes of all species. Interstitial telomeric sites were detected in three species belonging to the H. semilineatus group: H. boans, H. geographicus, and H. wavrini.

Conclusion: The results of this study reinforce the complexity previously observed within the genus Hypsiboas and in the different groups that compose this taxon. More studies are needed focusing on this group and covering larger sampling areas, especially in the Brazilian Amazon, to improve our understanding of this fascinating and complex group.

Show MeSH