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Erythrocyte stiffness during morphological remodeling induced by carbon ion radiation.

Zhang B, Liu B, Zhang H, Wang J - PLoS ONE (2014)

Bottom Line: The adverse effect induced by carbon ion radiation (CIR) is still an unavoidable hazard to the treatment object.Thus, evaluation of its adverse effects on the body is a critical problem with respect to radiation therapy.Additionally, for the first time, the current study provides a new strategy for enhancing the assessment of the curative effects and safety of clinical radiotherapy, as well as reducing adverse effects.

View Article: PubMed Central - PubMed

Affiliation: School of Civil Engineering and Mechanics, Lanzhou University, Lanzhou, 730000, PR China; Key Laboratory of Mechanics on Disaster and Environment in Western China, The Ministry of Education of China, Lanzhou University, 730000, PR China; Institute of Biomechanics and Medical Engineering, Lanzhou University, Lanzhou, 730000, PR China.

ABSTRACT
The adverse effect induced by carbon ion radiation (CIR) is still an unavoidable hazard to the treatment object. Thus, evaluation of its adverse effects on the body is a critical problem with respect to radiation therapy. We aimed to investigate the change between the configuration and mechanical properties of erythrocytes induced by radiation and found differences in both the configuration and the mechanical properties with involving in morphological remodeling process. Syrian hamsters were subjected to whole-body irradiation with carbon ion beams (1, 2, 4, and 6 Gy) or X-rays (2, 4, 6, and 12 Gy) for 3, 14 and 28 days. Erythrocytes in peripheral blood and bone marrow were collected for cytomorphological analysis. The mechanical properties of the erythrocytes were determined using atomic force microscopy, and the expression of the cytoskeletal protein spectrin-α1 was analyzed via western blotting. The results showed that dynamic changes were evident in erythrocytes exposed to different doses of carbon ion beams compared with X-rays and the control (0 Gy). The magnitude of impairment of the cell number and cellular morphology manifested the subtle variation according to the irradiation dose. In particular, the differences in the size, shape and mechanical properties of the erythrocytes were well exhibited. Furthermore, immunoblot data showed that the expression of the cytoskeletal protein spectrin-α1 was changed after irradiation, and there was a common pattern among its substantive characteristics in the irradiated group. Based on these findings, the present study concluded that CIR could induce a change in mechanical properties during morphological remodeling of erythrocytes. According to the unique characteristics of the biomechanical categories, we deduce that changes in cytomorphology and mechanical properties can be measured to evaluate the adverse effects generated by tumor radiotherapy. Additionally, for the first time, the current study provides a new strategy for enhancing the assessment of the curative effects and safety of clinical radiotherapy, as well as reducing adverse effects.

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The number of erythroid cells in BM accounted for the relative percentage of the total of BM cells after exposed carbon ion beams or X-rays.From the column chart showed that the pronormoblast and basophilic erythroblast seemed upward tendency with the increasing of absorbed dose in carbon ions radiation, but the change in X-rays radiation groups seemed to be not obvious, as for the polychromatophilic erythroblast and normoblast, the percentage of cells number had decreased significantly in two types of irradiation, and the differences between groups had significant statistical significance (p<0.05).
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pone-0112624-g004: The number of erythroid cells in BM accounted for the relative percentage of the total of BM cells after exposed carbon ion beams or X-rays.From the column chart showed that the pronormoblast and basophilic erythroblast seemed upward tendency with the increasing of absorbed dose in carbon ions radiation, but the change in X-rays radiation groups seemed to be not obvious, as for the polychromatophilic erythroblast and normoblast, the percentage of cells number had decreased significantly in two types of irradiation, and the differences between groups had significant statistical significance (p<0.05).

Mentions: Changes in the BM erythrocytes after irradiation were analyzed using a tangible cell-counting method. The purpose of our observations was to compare the characteristics among the different cell population types in the total number of BM cells and to understand the distribution characteristics of erythroid cells in BM caused by irradiation. The results revealed a mild myeloproliferative effect with small doses of radiation. Additionally, given the priority of the early development of erythroid cells, the pronormoblasts and basophilic erythroblasts seemed to demonstrate a tendency to increase with increasing absorbed doses of CIR; however, this change in the X-ray radiation group was not obvious. When the radiation dose increased, an obvious inhibitory effect was shown on BM. However, the proportions of polychromatophilic erythroblasts and normoblasts decreased significantly following both types of irradiation. In addition, the data indicated the presence of the myeloid maturation stage in a significantly higher proportion of granulocytes. Furthermore, the annular core, class changes and lack of particles were easily observed via the additive pleochroism of the erythrocytes (Figure 3 and Figure 4).


Erythrocyte stiffness during morphological remodeling induced by carbon ion radiation.

Zhang B, Liu B, Zhang H, Wang J - PLoS ONE (2014)

The number of erythroid cells in BM accounted for the relative percentage of the total of BM cells after exposed carbon ion beams or X-rays.From the column chart showed that the pronormoblast and basophilic erythroblast seemed upward tendency with the increasing of absorbed dose in carbon ions radiation, but the change in X-rays radiation groups seemed to be not obvious, as for the polychromatophilic erythroblast and normoblast, the percentage of cells number had decreased significantly in two types of irradiation, and the differences between groups had significant statistical significance (p<0.05).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4234377&req=5

pone-0112624-g004: The number of erythroid cells in BM accounted for the relative percentage of the total of BM cells after exposed carbon ion beams or X-rays.From the column chart showed that the pronormoblast and basophilic erythroblast seemed upward tendency with the increasing of absorbed dose in carbon ions radiation, but the change in X-rays radiation groups seemed to be not obvious, as for the polychromatophilic erythroblast and normoblast, the percentage of cells number had decreased significantly in two types of irradiation, and the differences between groups had significant statistical significance (p<0.05).
Mentions: Changes in the BM erythrocytes after irradiation were analyzed using a tangible cell-counting method. The purpose of our observations was to compare the characteristics among the different cell population types in the total number of BM cells and to understand the distribution characteristics of erythroid cells in BM caused by irradiation. The results revealed a mild myeloproliferative effect with small doses of radiation. Additionally, given the priority of the early development of erythroid cells, the pronormoblasts and basophilic erythroblasts seemed to demonstrate a tendency to increase with increasing absorbed doses of CIR; however, this change in the X-ray radiation group was not obvious. When the radiation dose increased, an obvious inhibitory effect was shown on BM. However, the proportions of polychromatophilic erythroblasts and normoblasts decreased significantly following both types of irradiation. In addition, the data indicated the presence of the myeloid maturation stage in a significantly higher proportion of granulocytes. Furthermore, the annular core, class changes and lack of particles were easily observed via the additive pleochroism of the erythrocytes (Figure 3 and Figure 4).

Bottom Line: The adverse effect induced by carbon ion radiation (CIR) is still an unavoidable hazard to the treatment object.Thus, evaluation of its adverse effects on the body is a critical problem with respect to radiation therapy.Additionally, for the first time, the current study provides a new strategy for enhancing the assessment of the curative effects and safety of clinical radiotherapy, as well as reducing adverse effects.

View Article: PubMed Central - PubMed

Affiliation: School of Civil Engineering and Mechanics, Lanzhou University, Lanzhou, 730000, PR China; Key Laboratory of Mechanics on Disaster and Environment in Western China, The Ministry of Education of China, Lanzhou University, 730000, PR China; Institute of Biomechanics and Medical Engineering, Lanzhou University, Lanzhou, 730000, PR China.

ABSTRACT
The adverse effect induced by carbon ion radiation (CIR) is still an unavoidable hazard to the treatment object. Thus, evaluation of its adverse effects on the body is a critical problem with respect to radiation therapy. We aimed to investigate the change between the configuration and mechanical properties of erythrocytes induced by radiation and found differences in both the configuration and the mechanical properties with involving in morphological remodeling process. Syrian hamsters were subjected to whole-body irradiation with carbon ion beams (1, 2, 4, and 6 Gy) or X-rays (2, 4, 6, and 12 Gy) for 3, 14 and 28 days. Erythrocytes in peripheral blood and bone marrow were collected for cytomorphological analysis. The mechanical properties of the erythrocytes were determined using atomic force microscopy, and the expression of the cytoskeletal protein spectrin-α1 was analyzed via western blotting. The results showed that dynamic changes were evident in erythrocytes exposed to different doses of carbon ion beams compared with X-rays and the control (0 Gy). The magnitude of impairment of the cell number and cellular morphology manifested the subtle variation according to the irradiation dose. In particular, the differences in the size, shape and mechanical properties of the erythrocytes were well exhibited. Furthermore, immunoblot data showed that the expression of the cytoskeletal protein spectrin-α1 was changed after irradiation, and there was a common pattern among its substantive characteristics in the irradiated group. Based on these findings, the present study concluded that CIR could induce a change in mechanical properties during morphological remodeling of erythrocytes. According to the unique characteristics of the biomechanical categories, we deduce that changes in cytomorphology and mechanical properties can be measured to evaluate the adverse effects generated by tumor radiotherapy. Additionally, for the first time, the current study provides a new strategy for enhancing the assessment of the curative effects and safety of clinical radiotherapy, as well as reducing adverse effects.

Show MeSH
Related in: MedlinePlus