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Automated sample-changing robot for solution scattering experiments at the EMBL Hamburg SAXS station X33.

Round AR, Franke D, Moritz S, Huchler R, Fritsche M, Malthan D, Klaering R, Svergun DI, Roessle M - J Appl Crystallogr (2008)

Bottom Line: At X33, a throughput of approximately 12 samples per hour, with a failure rate of sample loading of less than 0.5%, was observed.The feedback from users indicates that the ease of use and reliability of the user operation at the beamline were greatly improved compared with the manual filling mode.Full integration with the beamline control software, allowing for automated data collection of all samples loaded into the machine with remote control from the user, is presently being implemented.

View Article: PubMed Central - HTML - PubMed

Affiliation: EMBL Hamburg, Building 25a, Notkestrasse 85, 22603 Hamburg, Germany.

ABSTRACT
There is a rapidly increasing interest in the use of synchrotron small-angle X-ray scattering (SAXS) for large-scale studies of biological macromolecules in solution, and this requires an adequate means of automating the experiment. A prototype has been developed of an automated sample changer for solution SAXS, where the solutions are kept in thermostatically controlled well plates allowing for operation with up to 192 samples. The measuring protocol involves controlled loading of protein solutions and matching buffers, followed by cleaning and drying of the cell between measurements. The system was installed and tested at the X33 beamline of the EMBL, at the storage ring DORIS-III (DESY, Hamburg), where it was used by over 50 external groups during 2007. At X33, a throughput of approximately 12 samples per hour, with a failure rate of sample loading of less than 0.5%, was observed. The feedback from users indicates that the ease of use and reliability of the user operation at the beamline were greatly improved compared with the manual filling mode. The changer is controlled by a client-server-based network protocol, locally and remotely. During the testing phase, the changer was operated in an attended mode to assess its reliability and convenience. Full integration with the beamline control software, allowing for automated data collection of all samples loaded into the machine with remote control from the user, is presently being implemented. The approach reported is not limited to synchrotron-based SAXS but can also be used on laboratory and neutron sources.

No MeSH data available.


Comparison of 5 mg ml−1 BSA (bovine serum albumin) protein solution manually and automatically filled by the sample changer. The deviations are in the range of the experimental errors
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fig4: Comparison of 5 mg ml−1 BSA (bovine serum albumin) protein solution manually and automatically filled by the sample changer. The deviations are in the range of the experimental errors

Mentions: The automatic sample changer was put into test operation in Hamburg in August 2007 and was thoroughly tested offline before installing it at the X33 beamline. Comparison of SAXS patterns between manual filling and automated operation showed no differences (see Fig. 4 ▶). During the first three months of user operation in September–December 2007, over 50 international groups used the changer to measure over 2000 protein samples of various concentrations. A total of over 4500 individual SAXS images have been collected at the X33 beamline from the samples loaded by the device.


Automated sample-changing robot for solution scattering experiments at the EMBL Hamburg SAXS station X33.

Round AR, Franke D, Moritz S, Huchler R, Fritsche M, Malthan D, Klaering R, Svergun DI, Roessle M - J Appl Crystallogr (2008)

Comparison of 5 mg ml−1 BSA (bovine serum albumin) protein solution manually and automatically filled by the sample changer. The deviations are in the range of the experimental errors
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4233401&req=5

fig4: Comparison of 5 mg ml−1 BSA (bovine serum albumin) protein solution manually and automatically filled by the sample changer. The deviations are in the range of the experimental errors
Mentions: The automatic sample changer was put into test operation in Hamburg in August 2007 and was thoroughly tested offline before installing it at the X33 beamline. Comparison of SAXS patterns between manual filling and automated operation showed no differences (see Fig. 4 ▶). During the first three months of user operation in September–December 2007, over 50 international groups used the changer to measure over 2000 protein samples of various concentrations. A total of over 4500 individual SAXS images have been collected at the X33 beamline from the samples loaded by the device.

Bottom Line: At X33, a throughput of approximately 12 samples per hour, with a failure rate of sample loading of less than 0.5%, was observed.The feedback from users indicates that the ease of use and reliability of the user operation at the beamline were greatly improved compared with the manual filling mode.Full integration with the beamline control software, allowing for automated data collection of all samples loaded into the machine with remote control from the user, is presently being implemented.

View Article: PubMed Central - HTML - PubMed

Affiliation: EMBL Hamburg, Building 25a, Notkestrasse 85, 22603 Hamburg, Germany.

ABSTRACT
There is a rapidly increasing interest in the use of synchrotron small-angle X-ray scattering (SAXS) for large-scale studies of biological macromolecules in solution, and this requires an adequate means of automating the experiment. A prototype has been developed of an automated sample changer for solution SAXS, where the solutions are kept in thermostatically controlled well plates allowing for operation with up to 192 samples. The measuring protocol involves controlled loading of protein solutions and matching buffers, followed by cleaning and drying of the cell between measurements. The system was installed and tested at the X33 beamline of the EMBL, at the storage ring DORIS-III (DESY, Hamburg), where it was used by over 50 external groups during 2007. At X33, a throughput of approximately 12 samples per hour, with a failure rate of sample loading of less than 0.5%, was observed. The feedback from users indicates that the ease of use and reliability of the user operation at the beamline were greatly improved compared with the manual filling mode. The changer is controlled by a client-server-based network protocol, locally and remotely. During the testing phase, the changer was operated in an attended mode to assess its reliability and convenience. Full integration with the beamline control software, allowing for automated data collection of all samples loaded into the machine with remote control from the user, is presently being implemented. The approach reported is not limited to synchrotron-based SAXS but can also be used on laboratory and neutron sources.

No MeSH data available.