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Automated sample-changing robot for solution scattering experiments at the EMBL Hamburg SAXS station X33.

Round AR, Franke D, Moritz S, Huchler R, Fritsche M, Malthan D, Klaering R, Svergun DI, Roessle M - J Appl Crystallogr (2008)

Bottom Line: At X33, a throughput of approximately 12 samples per hour, with a failure rate of sample loading of less than 0.5%, was observed.The feedback from users indicates that the ease of use and reliability of the user operation at the beamline were greatly improved compared with the manual filling mode.Full integration with the beamline control software, allowing for automated data collection of all samples loaded into the machine with remote control from the user, is presently being implemented.

View Article: PubMed Central - HTML - PubMed

Affiliation: EMBL Hamburg, Building 25a, Notkestrasse 85, 22603 Hamburg, Germany.

ABSTRACT
There is a rapidly increasing interest in the use of synchrotron small-angle X-ray scattering (SAXS) for large-scale studies of biological macromolecules in solution, and this requires an adequate means of automating the experiment. A prototype has been developed of an automated sample changer for solution SAXS, where the solutions are kept in thermostatically controlled well plates allowing for operation with up to 192 samples. The measuring protocol involves controlled loading of protein solutions and matching buffers, followed by cleaning and drying of the cell between measurements. The system was installed and tested at the X33 beamline of the EMBL, at the storage ring DORIS-III (DESY, Hamburg), where it was used by over 50 external groups during 2007. At X33, a throughput of approximately 12 samples per hour, with a failure rate of sample loading of less than 0.5%, was observed. The feedback from users indicates that the ease of use and reliability of the user operation at the beamline were greatly improved compared with the manual filling mode. The changer is controlled by a client-server-based network protocol, locally and remotely. During the testing phase, the changer was operated in an attended mode to assess its reliability and convenience. Full integration with the beamline control software, allowing for automated data collection of all samples loaded into the machine with remote control from the user, is presently being implemented. The approach reported is not limited to synchrotron-based SAXS but can also be used on laboratory and neutron sources.

No MeSH data available.


(a) Automatic sample changer. (b) In operation at the X33 beamline. (1) Integrated touchscreen monitor, (2) motorized XYZ stage, (3) emergency stop buttons, (4) sample storage drawer, (5) in-vacuum sample measurement environment, (6) video monitor to observe sample measurement cell.
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fig1: (a) Automatic sample changer. (b) In operation at the X33 beamline. (1) Integrated touchscreen monitor, (2) motorized XYZ stage, (3) emergency stop buttons, (4) sample storage drawer, (5) in-vacuum sample measurement environment, (6) video monitor to observe sample measurement cell.

Mentions: The prototype device (Fig. 1 ▶) has been designed to fulfill all the tasks required to collect data according to the protocol in use at the X33 beamline. This bending-magnet beamline is dedicated to the measurement of solutions of biological macromolecules and has an in-vacuum sample compartment with flat mica or polycarbonate windows. The sample volume is about 40–45 µl and the typical exposure times are 1–2 min (Roessle et al., 2007 ▶). The measuring protocol involves the loading of protein solutions and matched buffers in an identical way so as not to introduce errors; this approach requires the cleaning and drying of the sample cell between measurements.


Automated sample-changing robot for solution scattering experiments at the EMBL Hamburg SAXS station X33.

Round AR, Franke D, Moritz S, Huchler R, Fritsche M, Malthan D, Klaering R, Svergun DI, Roessle M - J Appl Crystallogr (2008)

(a) Automatic sample changer. (b) In operation at the X33 beamline. (1) Integrated touchscreen monitor, (2) motorized XYZ stage, (3) emergency stop buttons, (4) sample storage drawer, (5) in-vacuum sample measurement environment, (6) video monitor to observe sample measurement cell.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4233401&req=5

fig1: (a) Automatic sample changer. (b) In operation at the X33 beamline. (1) Integrated touchscreen monitor, (2) motorized XYZ stage, (3) emergency stop buttons, (4) sample storage drawer, (5) in-vacuum sample measurement environment, (6) video monitor to observe sample measurement cell.
Mentions: The prototype device (Fig. 1 ▶) has been designed to fulfill all the tasks required to collect data according to the protocol in use at the X33 beamline. This bending-magnet beamline is dedicated to the measurement of solutions of biological macromolecules and has an in-vacuum sample compartment with flat mica or polycarbonate windows. The sample volume is about 40–45 µl and the typical exposure times are 1–2 min (Roessle et al., 2007 ▶). The measuring protocol involves the loading of protein solutions and matched buffers in an identical way so as not to introduce errors; this approach requires the cleaning and drying of the sample cell between measurements.

Bottom Line: At X33, a throughput of approximately 12 samples per hour, with a failure rate of sample loading of less than 0.5%, was observed.The feedback from users indicates that the ease of use and reliability of the user operation at the beamline were greatly improved compared with the manual filling mode.Full integration with the beamline control software, allowing for automated data collection of all samples loaded into the machine with remote control from the user, is presently being implemented.

View Article: PubMed Central - HTML - PubMed

Affiliation: EMBL Hamburg, Building 25a, Notkestrasse 85, 22603 Hamburg, Germany.

ABSTRACT
There is a rapidly increasing interest in the use of synchrotron small-angle X-ray scattering (SAXS) for large-scale studies of biological macromolecules in solution, and this requires an adequate means of automating the experiment. A prototype has been developed of an automated sample changer for solution SAXS, where the solutions are kept in thermostatically controlled well plates allowing for operation with up to 192 samples. The measuring protocol involves controlled loading of protein solutions and matching buffers, followed by cleaning and drying of the cell between measurements. The system was installed and tested at the X33 beamline of the EMBL, at the storage ring DORIS-III (DESY, Hamburg), where it was used by over 50 external groups during 2007. At X33, a throughput of approximately 12 samples per hour, with a failure rate of sample loading of less than 0.5%, was observed. The feedback from users indicates that the ease of use and reliability of the user operation at the beamline were greatly improved compared with the manual filling mode. The changer is controlled by a client-server-based network protocol, locally and remotely. During the testing phase, the changer was operated in an attended mode to assess its reliability and convenience. Full integration with the beamline control software, allowing for automated data collection of all samples loaded into the machine with remote control from the user, is presently being implemented. The approach reported is not limited to synchrotron-based SAXS but can also be used on laboratory and neutron sources.

No MeSH data available.