Limits...
Investigating dynamic structural and mechanical changes of neuroblastoma cells associated with glutamate-mediated neurodegeneration.

Fang Y, Iu CY, Lui CN, Zou Y, Fung CK, Li HW, Xi N, Yung KK, Lai KW - Sci Rep (2014)

Bottom Line: Here, we investigate the real-time dynamic structural and mechanical changes associated with the neurodegeneration induced by the activation of N-methyl-D-aspartate (NMDA) receptors (a subtype of glutamate receptors) at the nanoscale.A significant increase in surface roughness and stiffness of the cell is observed after NMDA treatment, which indicates the time-dependent neuronal cell behavior under NMDA-mediated neurodegeneration.The present AFM based study further advance our understanding of the neurodegenerative process to elucidate the pathways and mechanisms that govern NMDA induced neurodegeneration, so as to facilitate the development of novel therapeutic strategies for neurodegenerative diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong.

ABSTRACT
Glutamate-mediated neurodegeneration resulting from excessive activation of glutamate receptors is recognized as one of the major causes of various neurological disorders such as Alzheimer's and Huntington's diseases. However, the underlying mechanisms in the neurodegenerative process remain unidentified. Here, we investigate the real-time dynamic structural and mechanical changes associated with the neurodegeneration induced by the activation of N-methyl-D-aspartate (NMDA) receptors (a subtype of glutamate receptors) at the nanoscale. Atomic force microscopy (AFM) is employed to measure the three-dimensional (3-D) topography and mechanical properties of live SH-SY5Y cells under stimulus of NMDA receptors. A significant increase in surface roughness and stiffness of the cell is observed after NMDA treatment, which indicates the time-dependent neuronal cell behavior under NMDA-mediated neurodegeneration. The present AFM based study further advance our understanding of the neurodegenerative process to elucidate the pathways and mechanisms that govern NMDA induced neurodegeneration, so as to facilitate the development of novel therapeutic strategies for neurodegenerative diseases.

Show MeSH

Related in: MedlinePlus

Representative AFM images of normal SH-SY5Y cells.(A) AFM height image and (B) height profile across the section line as marked in (A). The height of the cell is approximately 3 μm. (C) AFM deformation image and (D) deformation profile across the section line as marked in (C). The average deformation of the cell is about 250 nm. (scale bar: 10 µm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4233341&req=5

f2: Representative AFM images of normal SH-SY5Y cells.(A) AFM height image and (B) height profile across the section line as marked in (A). The height of the cell is approximately 3 μm. (C) AFM deformation image and (D) deformation profile across the section line as marked in (C). The average deformation of the cell is about 250 nm. (scale bar: 10 µm).

Mentions: A representative AFM height image of live SH-SY5Y neuronal cells was obtained (Fig. 2A) and the corresponding cross-sectional height profile indicates that the height of the cell was approximately 3 μm (Fig. 2B). Since the cell was relatively soft, the cell membrane was deformed by the AFM tip during the scanning and the deformation information of the entire cell was obtained from the deformation image and the cross-sectional deformation profile (Fig. 2C, D). The average deformation of the cell was about 250 nm.


Investigating dynamic structural and mechanical changes of neuroblastoma cells associated with glutamate-mediated neurodegeneration.

Fang Y, Iu CY, Lui CN, Zou Y, Fung CK, Li HW, Xi N, Yung KK, Lai KW - Sci Rep (2014)

Representative AFM images of normal SH-SY5Y cells.(A) AFM height image and (B) height profile across the section line as marked in (A). The height of the cell is approximately 3 μm. (C) AFM deformation image and (D) deformation profile across the section line as marked in (C). The average deformation of the cell is about 250 nm. (scale bar: 10 µm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4233341&req=5

f2: Representative AFM images of normal SH-SY5Y cells.(A) AFM height image and (B) height profile across the section line as marked in (A). The height of the cell is approximately 3 μm. (C) AFM deformation image and (D) deformation profile across the section line as marked in (C). The average deformation of the cell is about 250 nm. (scale bar: 10 µm).
Mentions: A representative AFM height image of live SH-SY5Y neuronal cells was obtained (Fig. 2A) and the corresponding cross-sectional height profile indicates that the height of the cell was approximately 3 μm (Fig. 2B). Since the cell was relatively soft, the cell membrane was deformed by the AFM tip during the scanning and the deformation information of the entire cell was obtained from the deformation image and the cross-sectional deformation profile (Fig. 2C, D). The average deformation of the cell was about 250 nm.

Bottom Line: Here, we investigate the real-time dynamic structural and mechanical changes associated with the neurodegeneration induced by the activation of N-methyl-D-aspartate (NMDA) receptors (a subtype of glutamate receptors) at the nanoscale.A significant increase in surface roughness and stiffness of the cell is observed after NMDA treatment, which indicates the time-dependent neuronal cell behavior under NMDA-mediated neurodegeneration.The present AFM based study further advance our understanding of the neurodegenerative process to elucidate the pathways and mechanisms that govern NMDA induced neurodegeneration, so as to facilitate the development of novel therapeutic strategies for neurodegenerative diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong.

ABSTRACT
Glutamate-mediated neurodegeneration resulting from excessive activation of glutamate receptors is recognized as one of the major causes of various neurological disorders such as Alzheimer's and Huntington's diseases. However, the underlying mechanisms in the neurodegenerative process remain unidentified. Here, we investigate the real-time dynamic structural and mechanical changes associated with the neurodegeneration induced by the activation of N-methyl-D-aspartate (NMDA) receptors (a subtype of glutamate receptors) at the nanoscale. Atomic force microscopy (AFM) is employed to measure the three-dimensional (3-D) topography and mechanical properties of live SH-SY5Y cells under stimulus of NMDA receptors. A significant increase in surface roughness and stiffness of the cell is observed after NMDA treatment, which indicates the time-dependent neuronal cell behavior under NMDA-mediated neurodegeneration. The present AFM based study further advance our understanding of the neurodegenerative process to elucidate the pathways and mechanisms that govern NMDA induced neurodegeneration, so as to facilitate the development of novel therapeutic strategies for neurodegenerative diseases.

Show MeSH
Related in: MedlinePlus