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High-throughput formation of lipid bilayer membrane arrays with an asymmetric lipid composition.

Watanabe R, Soga N, Yamanaka T, Noji H - Sci Rep (2014)

Bottom Line: We present a micro-device in which more than 10,000 asymmetric lipid bilayer membranes are formed at a time on micro-chamber arrays.The arrayed asymmetric lipid bilayers, where lipid compositions are different between the inner and outer leaflets, are formed with high efficiency of over 97% by injecting several types of liquids into a micro-device that has hydrophilic-in-hydrophobic surfaces.The lipid compositional asymmetry is an intrinsic property of bio-membranes, and therefore, this micro-device extends the versatility of artificial lipid-bilayer systems, which were previously limited to symmetric bilayer formation, and could contribute to the understanding of the role of lipid compositional asymmetry in cell physiology and also to further analytical and pharmacological applications.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan [2] PRESTO, JST, Bunkyo-ku, Tokyo 113-8656, Japan.

ABSTRACT
We present a micro-device in which more than 10,000 asymmetric lipid bilayer membranes are formed at a time on micro-chamber arrays. The arrayed asymmetric lipid bilayers, where lipid compositions are different between the inner and outer leaflets, are formed with high efficiency of over 97% by injecting several types of liquids into a micro-device that has hydrophilic-in-hydrophobic surfaces. The lipid compositional asymmetry is an intrinsic property of bio-membranes, and therefore, this micro-device extends the versatility of artificial lipid-bilayer systems, which were previously limited to symmetric bilayer formation, and could contribute to the understanding of the role of lipid compositional asymmetry in cell physiology and also to further analytical and pharmacological applications.

No MeSH data available.


Related in: MedlinePlus

Micro-chamber arrays for asymmetric lipid bilayer formation.(a) Fabricated micro-device where the substrate of hydrophilic-in-hydrophobic structures is assembled with cover glass that has an access port for sample injection. (b) Bright field image of the through-hole structures of the carbon-fluorine hydrophobic polymer on a hydrophilic glass substrate. (c) Illustration of a fabricated structure (diameter, 4 μm; thickness, 500 nm).
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f1: Micro-chamber arrays for asymmetric lipid bilayer formation.(a) Fabricated micro-device where the substrate of hydrophilic-in-hydrophobic structures is assembled with cover glass that has an access port for sample injection. (b) Bright field image of the through-hole structures of the carbon-fluorine hydrophobic polymer on a hydrophilic glass substrate. (c) Illustration of a fabricated structure (diameter, 4 μm; thickness, 500 nm).

Mentions: Using conventional soft lithography, we fabricated a micro-device with more than 10,000 through-hole structures (7 fL, Φ = 4 μm, and h = 0.5 μm) on a carbon-fluorine hydrophobic polymer (CYTOP, Asahi-glass, Japan) on a hydrophilic glass substrate9101112 (Fig. 1). Because the fabrication process was well established in our previous studies101112, we are able to efficiently fabricate the micro-device, achieving a fabrication success rate of around 100%8. The through-hole structures were utilized as micro-chambers, whose orifice is suitable to mechanically support the lipid membrane because of its hydrophobicity8. Teflon has high hydrophobicity and has thus frequently been used as a support medium for lipid membranes in previous studies8. For exchange of the sample solution, a flow channel was constructed from the micro-chamber array patterned on a glass substrate, a spacer sheet, and a glass block, which had an access port for sample injection (Fig. 1a).


High-throughput formation of lipid bilayer membrane arrays with an asymmetric lipid composition.

Watanabe R, Soga N, Yamanaka T, Noji H - Sci Rep (2014)

Micro-chamber arrays for asymmetric lipid bilayer formation.(a) Fabricated micro-device where the substrate of hydrophilic-in-hydrophobic structures is assembled with cover glass that has an access port for sample injection. (b) Bright field image of the through-hole structures of the carbon-fluorine hydrophobic polymer on a hydrophilic glass substrate. (c) Illustration of a fabricated structure (diameter, 4 μm; thickness, 500 nm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4233334&req=5

f1: Micro-chamber arrays for asymmetric lipid bilayer formation.(a) Fabricated micro-device where the substrate of hydrophilic-in-hydrophobic structures is assembled with cover glass that has an access port for sample injection. (b) Bright field image of the through-hole structures of the carbon-fluorine hydrophobic polymer on a hydrophilic glass substrate. (c) Illustration of a fabricated structure (diameter, 4 μm; thickness, 500 nm).
Mentions: Using conventional soft lithography, we fabricated a micro-device with more than 10,000 through-hole structures (7 fL, Φ = 4 μm, and h = 0.5 μm) on a carbon-fluorine hydrophobic polymer (CYTOP, Asahi-glass, Japan) on a hydrophilic glass substrate9101112 (Fig. 1). Because the fabrication process was well established in our previous studies101112, we are able to efficiently fabricate the micro-device, achieving a fabrication success rate of around 100%8. The through-hole structures were utilized as micro-chambers, whose orifice is suitable to mechanically support the lipid membrane because of its hydrophobicity8. Teflon has high hydrophobicity and has thus frequently been used as a support medium for lipid membranes in previous studies8. For exchange of the sample solution, a flow channel was constructed from the micro-chamber array patterned on a glass substrate, a spacer sheet, and a glass block, which had an access port for sample injection (Fig. 1a).

Bottom Line: We present a micro-device in which more than 10,000 asymmetric lipid bilayer membranes are formed at a time on micro-chamber arrays.The arrayed asymmetric lipid bilayers, where lipid compositions are different between the inner and outer leaflets, are formed with high efficiency of over 97% by injecting several types of liquids into a micro-device that has hydrophilic-in-hydrophobic surfaces.The lipid compositional asymmetry is an intrinsic property of bio-membranes, and therefore, this micro-device extends the versatility of artificial lipid-bilayer systems, which were previously limited to symmetric bilayer formation, and could contribute to the understanding of the role of lipid compositional asymmetry in cell physiology and also to further analytical and pharmacological applications.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan [2] PRESTO, JST, Bunkyo-ku, Tokyo 113-8656, Japan.

ABSTRACT
We present a micro-device in which more than 10,000 asymmetric lipid bilayer membranes are formed at a time on micro-chamber arrays. The arrayed asymmetric lipid bilayers, where lipid compositions are different between the inner and outer leaflets, are formed with high efficiency of over 97% by injecting several types of liquids into a micro-device that has hydrophilic-in-hydrophobic surfaces. The lipid compositional asymmetry is an intrinsic property of bio-membranes, and therefore, this micro-device extends the versatility of artificial lipid-bilayer systems, which were previously limited to symmetric bilayer formation, and could contribute to the understanding of the role of lipid compositional asymmetry in cell physiology and also to further analytical and pharmacological applications.

No MeSH data available.


Related in: MedlinePlus