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The effect of poloxamer 407-based hydrogel on the osteoinductivity of demineralized bone matrix.

Lee JH, Baek HR, Lee KM, Lee HK, Im SB, Kim YS, Lee JH, Chang BS, Lee CK - Clin Orthop Surg (2014)

Bottom Line: Both of the compositions were applied to human mesenchymal stem cell (MSC) cultures, and monitored for alkaline phosphatase (ALP) staining and ALP activity.The DBM-W group showed stronger ALP staining at 7, 14, and 21 days of treatment, and significantly higher ALP activity at 7 and 14 days of treatment, compared to the DBM-H group.Decalcified histology found bony tissue with lamellation in both groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea. ; Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, Seoul, Korea.

ABSTRACT

Background: Demineralized bone matrix (DBM) is used for bone healing due to its osteoinductivity, but it requires a carrier for clinical application. Here, we report the effects on the osteoinductivity of DBM by use of a poloxamer 407-based hydrogel as the carrier, compared to sterile water.

Methods: DBM-W and DBM-H represent 27 wt% of DBM with sterile water and DBM with a poloxamer 407-based hydrogel, respectively. Both of the compositions were applied to human mesenchymal stem cell (MSC) cultures, and monitored for alkaline phosphatase (ALP) staining and ALP activity. Six 10-week-old athymic nude rats were used for abdominal muscle grafting with either DBM-W or DBM-H, and were tested by plane radiography, microfocus X-ray computed tomography (CT), and decalcified histology to evaluate ectopic bone formation.

Results: The DBM-W group showed stronger ALP staining at 7, 14, and 21 days of treatment, and significantly higher ALP activity at 7 and 14 days of treatment, compared to the DBM-H group. Plane radiography could not confirm the radio-opaque lesions in the rat ectopic bone formulation model. However, ectopic bone formation was observed in both groups by micro-CT. Compared to the DBM-H group, the DBM-W group showed higher bone volume, percent bone volume and trabecular number, and the difference in percent bone volume was statistically significant. Decalcified histology found bony tissue with lamellation in both groups.

Conclusions: Our results suggest that poloxamer 407-based hydrogel has efficacy as a DBM carrier since it shows ectopic bone formation, but its effects on the quality and quantity of osteoblastic differentiation in rat abdominal ectopic bone and MSC are considered negative.

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Related in: MedlinePlus

Microfocus X-ray computed tomography of a specimen from the DBM-W group (A) and DBM-H group (B) after 8 weeks of implantation. For both A and B, each panel represents a transaxial image, sagittal image, and coronal image (from left to right). DBM: demineralized bone matrix, DBM-W: DBM with sterile water, DBM-H: DBM with poloxamer 407-based hydrogel.
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Figure 4: Microfocus X-ray computed tomography of a specimen from the DBM-W group (A) and DBM-H group (B) after 8 weeks of implantation. For both A and B, each panel represents a transaxial image, sagittal image, and coronal image (from left to right). DBM: demineralized bone matrix, DBM-W: DBM with sterile water, DBM-H: DBM with poloxamer 407-based hydrogel.

Mentions: Plane radiography could not detect any clear radio-opaque lesions in either of the groups (Fig. 3). However, micro-CT confirmed distinct calcified tissues in both of the groups by coronal, axial and sagittal imaging (Fig. 4). Quantitative analysis further revealed that the DBM-W group had a higher bone volume and trabecular number, but with no statistical significance. However, the DBM-W group had a significantly higher percentage of bone volume (p = 0.015) than the DBM-H group (Table 1).


The effect of poloxamer 407-based hydrogel on the osteoinductivity of demineralized bone matrix.

Lee JH, Baek HR, Lee KM, Lee HK, Im SB, Kim YS, Lee JH, Chang BS, Lee CK - Clin Orthop Surg (2014)

Microfocus X-ray computed tomography of a specimen from the DBM-W group (A) and DBM-H group (B) after 8 weeks of implantation. For both A and B, each panel represents a transaxial image, sagittal image, and coronal image (from left to right). DBM: demineralized bone matrix, DBM-W: DBM with sterile water, DBM-H: DBM with poloxamer 407-based hydrogel.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4233226&req=5

Figure 4: Microfocus X-ray computed tomography of a specimen from the DBM-W group (A) and DBM-H group (B) after 8 weeks of implantation. For both A and B, each panel represents a transaxial image, sagittal image, and coronal image (from left to right). DBM: demineralized bone matrix, DBM-W: DBM with sterile water, DBM-H: DBM with poloxamer 407-based hydrogel.
Mentions: Plane radiography could not detect any clear radio-opaque lesions in either of the groups (Fig. 3). However, micro-CT confirmed distinct calcified tissues in both of the groups by coronal, axial and sagittal imaging (Fig. 4). Quantitative analysis further revealed that the DBM-W group had a higher bone volume and trabecular number, but with no statistical significance. However, the DBM-W group had a significantly higher percentage of bone volume (p = 0.015) than the DBM-H group (Table 1).

Bottom Line: Both of the compositions were applied to human mesenchymal stem cell (MSC) cultures, and monitored for alkaline phosphatase (ALP) staining and ALP activity.The DBM-W group showed stronger ALP staining at 7, 14, and 21 days of treatment, and significantly higher ALP activity at 7 and 14 days of treatment, compared to the DBM-H group.Decalcified histology found bony tissue with lamellation in both groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, SMG-SNU Boramae Medical Center, Seoul National University College of Medicine, Seoul, Korea. ; Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, Seoul, Korea.

ABSTRACT

Background: Demineralized bone matrix (DBM) is used for bone healing due to its osteoinductivity, but it requires a carrier for clinical application. Here, we report the effects on the osteoinductivity of DBM by use of a poloxamer 407-based hydrogel as the carrier, compared to sterile water.

Methods: DBM-W and DBM-H represent 27 wt% of DBM with sterile water and DBM with a poloxamer 407-based hydrogel, respectively. Both of the compositions were applied to human mesenchymal stem cell (MSC) cultures, and monitored for alkaline phosphatase (ALP) staining and ALP activity. Six 10-week-old athymic nude rats were used for abdominal muscle grafting with either DBM-W or DBM-H, and were tested by plane radiography, microfocus X-ray computed tomography (CT), and decalcified histology to evaluate ectopic bone formation.

Results: The DBM-W group showed stronger ALP staining at 7, 14, and 21 days of treatment, and significantly higher ALP activity at 7 and 14 days of treatment, compared to the DBM-H group. Plane radiography could not confirm the radio-opaque lesions in the rat ectopic bone formulation model. However, ectopic bone formation was observed in both groups by micro-CT. Compared to the DBM-H group, the DBM-W group showed higher bone volume, percent bone volume and trabecular number, and the difference in percent bone volume was statistically significant. Decalcified histology found bony tissue with lamellation in both groups.

Conclusions: Our results suggest that poloxamer 407-based hydrogel has efficacy as a DBM carrier since it shows ectopic bone formation, but its effects on the quality and quantity of osteoblastic differentiation in rat abdominal ectopic bone and MSC are considered negative.

Show MeSH
Related in: MedlinePlus