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Role of matrix metalloproteinase (MMP) 2 and MMP-9 in soft tissue sarcoma.

Yang HK, Jeong KC, Kim YK, Jung ST - Clin Orthop Surg (2014)

Bottom Line: By Western blot analysis, the expression levels of MMP-2, TIMP-1, and TIMP-2 were higher in the metastatic group (p < 0.05), but MMP-9 showed only a slight increase in the metastatic group compared with the non-metastatic group (p > 0.05).Finally, gelatin zymography analysis showed that the expression levels of the pro- and active forms of MMP-2 were significantly higher in the metastatic group (p < 0.05), but the expression of the pro- and active forms of MMP-9 showed a slight decrease in the metastatic group (p > 0.05).These results suggest that MMP-2, MMP-9, TIMP-1, and TIMP-2 may have important roles in the development and progression of MFH, and that the degree of expression of these metalloproteinases and their inhibitors, especially MMP-2, could be useful as prognostic factors related to metastasis in MFH.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Chonnam National University Medical School, Gwangju, Korea.

ABSTRACT

Background: We investigated the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in malignant fibrous histiocytoma (MFH), and determined whether these could be useful as prognostic factors.

Methods: Among patients treated from 1993 to 2007, 30 cases of MFH were evaluated. Immunohistochemical staining was performed for MMP-2, MMP-9, TIMP-1, and TIMP-2 using paraffin wax-embedded blocks of MFH tissues. Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot and zymography were performed using fresh tissues obtained from 17 of the 30 cases. The levels of MMP and TIMP expression were compared between the MFH and normal control groups, and between non-metastatic and metastatic MFH groups.

Results: Expression levels of MMP-2, MMP-9, TIMP-1, and TIMP-2 were higher in the MFH group than the control group by RT-PCR, Western blotting, and zymography. Immunohistochemical staining revealed that MMP-2 and MMP-9 protein expression was higher in the metastatic than in the non-metastatic group. The expression levels of MMP-2 and TIMP-1 were significantly higher in the metastatic than in the non-metastatic group (p < 0.05) by RT-PCR. By Western blot analysis, the expression levels of MMP-2, TIMP-1, and TIMP-2 were higher in the metastatic group (p < 0.05), but MMP-9 showed only a slight increase in the metastatic group compared with the non-metastatic group (p > 0.05). Finally, gelatin zymography analysis showed that the expression levels of the pro- and active forms of MMP-2 were significantly higher in the metastatic group (p < 0.05), but the expression of the pro- and active forms of MMP-9 showed a slight decrease in the metastatic group (p > 0.05).

Conclusions: These results suggest that MMP-2, MMP-9, TIMP-1, and TIMP-2 may have important roles in the development and progression of MFH, and that the degree of expression of these metalloproteinases and their inhibitors, especially MMP-2, could be useful as prognostic factors related to metastasis in MFH.

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Western blotting results for matrix metalloproteinase (MMP) 2, MMP-9, tissue inhibitors of metalloproteinase (TIMP) 1, and TIMP-2. The expression levels of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the non-metastatic malignant fibrous histiocytoma (MFH) and metastatic MFH groups were higher than those in the control group, and these results were significant for MMP-9 and TIMP-2 (p < 0.05). The expression levels of MMP-2, TIMP-1, and TIMP-2 were higher in the metastatic MFH group than in the non-metastatic MFH group (p < 0.05). Meta: metastatic. *Significant, Mann-Whitney U-test, p < 0.05.
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Figure 3: Western blotting results for matrix metalloproteinase (MMP) 2, MMP-9, tissue inhibitors of metalloproteinase (TIMP) 1, and TIMP-2. The expression levels of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the non-metastatic malignant fibrous histiocytoma (MFH) and metastatic MFH groups were higher than those in the control group, and these results were significant for MMP-9 and TIMP-2 (p < 0.05). The expression levels of MMP-2, TIMP-1, and TIMP-2 were higher in the metastatic MFH group than in the non-metastatic MFH group (p < 0.05). Meta: metastatic. *Significant, Mann-Whitney U-test, p < 0.05.

Mentions: The Western blotting results also showed that the expressions of MMP-2, MMP-9, TIMP-1, and TIMP-2 were higher in the non-metastatic and metastatic groups than in the control group, and these results were significant for MMP-9 and TIMP-2 (p < 0.05). The expression levels of MMP-2, TIMP-1, and TIMP-2 were significantly higher in the metastatic group than the non-metastatic group (p < 0.05), but there was no statistically significant difference in the expression of MMP-9 between two groups (p > 0.05) (Fig. 3).


Role of matrix metalloproteinase (MMP) 2 and MMP-9 in soft tissue sarcoma.

Yang HK, Jeong KC, Kim YK, Jung ST - Clin Orthop Surg (2014)

Western blotting results for matrix metalloproteinase (MMP) 2, MMP-9, tissue inhibitors of metalloproteinase (TIMP) 1, and TIMP-2. The expression levels of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the non-metastatic malignant fibrous histiocytoma (MFH) and metastatic MFH groups were higher than those in the control group, and these results were significant for MMP-9 and TIMP-2 (p < 0.05). The expression levels of MMP-2, TIMP-1, and TIMP-2 were higher in the metastatic MFH group than in the non-metastatic MFH group (p < 0.05). Meta: metastatic. *Significant, Mann-Whitney U-test, p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4233225&req=5

Figure 3: Western blotting results for matrix metalloproteinase (MMP) 2, MMP-9, tissue inhibitors of metalloproteinase (TIMP) 1, and TIMP-2. The expression levels of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the non-metastatic malignant fibrous histiocytoma (MFH) and metastatic MFH groups were higher than those in the control group, and these results were significant for MMP-9 and TIMP-2 (p < 0.05). The expression levels of MMP-2, TIMP-1, and TIMP-2 were higher in the metastatic MFH group than in the non-metastatic MFH group (p < 0.05). Meta: metastatic. *Significant, Mann-Whitney U-test, p < 0.05.
Mentions: The Western blotting results also showed that the expressions of MMP-2, MMP-9, TIMP-1, and TIMP-2 were higher in the non-metastatic and metastatic groups than in the control group, and these results were significant for MMP-9 and TIMP-2 (p < 0.05). The expression levels of MMP-2, TIMP-1, and TIMP-2 were significantly higher in the metastatic group than the non-metastatic group (p < 0.05), but there was no statistically significant difference in the expression of MMP-9 between two groups (p > 0.05) (Fig. 3).

Bottom Line: By Western blot analysis, the expression levels of MMP-2, TIMP-1, and TIMP-2 were higher in the metastatic group (p < 0.05), but MMP-9 showed only a slight increase in the metastatic group compared with the non-metastatic group (p > 0.05).Finally, gelatin zymography analysis showed that the expression levels of the pro- and active forms of MMP-2 were significantly higher in the metastatic group (p < 0.05), but the expression of the pro- and active forms of MMP-9 showed a slight decrease in the metastatic group (p > 0.05).These results suggest that MMP-2, MMP-9, TIMP-1, and TIMP-2 may have important roles in the development and progression of MFH, and that the degree of expression of these metalloproteinases and their inhibitors, especially MMP-2, could be useful as prognostic factors related to metastasis in MFH.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Chonnam National University Medical School, Gwangju, Korea.

ABSTRACT

Background: We investigated the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in malignant fibrous histiocytoma (MFH), and determined whether these could be useful as prognostic factors.

Methods: Among patients treated from 1993 to 2007, 30 cases of MFH were evaluated. Immunohistochemical staining was performed for MMP-2, MMP-9, TIMP-1, and TIMP-2 using paraffin wax-embedded blocks of MFH tissues. Reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot and zymography were performed using fresh tissues obtained from 17 of the 30 cases. The levels of MMP and TIMP expression were compared between the MFH and normal control groups, and between non-metastatic and metastatic MFH groups.

Results: Expression levels of MMP-2, MMP-9, TIMP-1, and TIMP-2 were higher in the MFH group than the control group by RT-PCR, Western blotting, and zymography. Immunohistochemical staining revealed that MMP-2 and MMP-9 protein expression was higher in the metastatic than in the non-metastatic group. The expression levels of MMP-2 and TIMP-1 were significantly higher in the metastatic than in the non-metastatic group (p < 0.05) by RT-PCR. By Western blot analysis, the expression levels of MMP-2, TIMP-1, and TIMP-2 were higher in the metastatic group (p < 0.05), but MMP-9 showed only a slight increase in the metastatic group compared with the non-metastatic group (p > 0.05). Finally, gelatin zymography analysis showed that the expression levels of the pro- and active forms of MMP-2 were significantly higher in the metastatic group (p < 0.05), but the expression of the pro- and active forms of MMP-9 showed a slight decrease in the metastatic group (p > 0.05).

Conclusions: These results suggest that MMP-2, MMP-9, TIMP-1, and TIMP-2 may have important roles in the development and progression of MFH, and that the degree of expression of these metalloproteinases and their inhibitors, especially MMP-2, could be useful as prognostic factors related to metastasis in MFH.

Show MeSH
Related in: MedlinePlus