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Identification of the mulberry genes involved in ethylene biosynthesis and signaling pathways and the expression of MaERF-B2-1 and MaERF-B2-2 in the response to flooding stress.

Shang J, Song P, Ma B, Qi X, Zeng Q, Xiang Z, He N - Funct. Integr. Genomics (2014)

Bottom Line: A total of 145 mulberry genes were identified by both homology-based and hidden Markov model (HMM) search, including 29 genes associated with ethylene biosynthesis and 116 genes in the AP2/ERF family.The differences in gene expression were also observed in different tissues.The expression of two mulberry genes in the AP2/ERF family, MaERF-B2-1 and MaERF-B2-2, was found to be associated with the response to flooding stress.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing, 400715, China.

ABSTRACT
The phytohormone ethylene is essential to plant growth and development. It plays crucial roles in responses to biotic and abiotic stress. The mulberry tree is an important crop plant in countries in which people rear silkworms for silk production. The availability of the mulberry genome has made it possible to identify mulberry genes involved in ethylene biosynthesis and signal pathways. A total of 145 mulberry genes were identified by both homology-based and hidden Markov model (HMM) search, including 29 genes associated with ethylene biosynthesis and 116 genes in the AP2/ERF family. Studies on gene structure have provided a genetic basis for understanding the functions of these genes. The differences in gene expression were also observed in different tissues. The expression of two mulberry genes in the AP2/ERF family, MaERF-B2-1 and MaERF-B2-2, was found to be associated with the response to flooding stress.

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Expression patterns of 18 genes involved in the ethylene biosynthesis and signal transduction pathways. Six tissues were used. Relative levels of gene expression by qRT-PCR were normalized against the mulberry ribosomal protein gene Morus024083. Data are represented as mean ± standard error of three replicates
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Fig2: Expression patterns of 18 genes involved in the ethylene biosynthesis and signal transduction pathways. Six tissues were used. Relative levels of gene expression by qRT-PCR were normalized against the mulberry ribosomal protein gene Morus024083. Data are represented as mean ± standard error of three replicates

Mentions: Genes whose expression was detected by qPCR are listed in Fig. 2. Results showed that the genes in these four families have diverse expression profiles across different tissues. MnACS2, MnACO2, MnACO3, MnETR2, MnEIN3, and MnEIL1 have higher levels of expression. On the contrary, the expression of MnACS3, MnACS4, MnACO4, MnERS1, MnEIN4, MnEIL3, and MnEIL4 was relatively repressed. In addition, MnACS5 exhibited tissue-specific expression in female flowers. Among ACO genes, MnACO1 and MnACO2 exhibited tissue-biased expression in fruit, although the expression levels of MnACO1 were only about one tenth of those of MnACO2. MnEIN3 and MnEIL1 showed more expression than the rest of the EIN3 family in roots and fruits.Fig. 2


Identification of the mulberry genes involved in ethylene biosynthesis and signaling pathways and the expression of MaERF-B2-1 and MaERF-B2-2 in the response to flooding stress.

Shang J, Song P, Ma B, Qi X, Zeng Q, Xiang Z, He N - Funct. Integr. Genomics (2014)

Expression patterns of 18 genes involved in the ethylene biosynthesis and signal transduction pathways. Six tissues were used. Relative levels of gene expression by qRT-PCR were normalized against the mulberry ribosomal protein gene Morus024083. Data are represented as mean ± standard error of three replicates
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4233114&req=5

Fig2: Expression patterns of 18 genes involved in the ethylene biosynthesis and signal transduction pathways. Six tissues were used. Relative levels of gene expression by qRT-PCR were normalized against the mulberry ribosomal protein gene Morus024083. Data are represented as mean ± standard error of three replicates
Mentions: Genes whose expression was detected by qPCR are listed in Fig. 2. Results showed that the genes in these four families have diverse expression profiles across different tissues. MnACS2, MnACO2, MnACO3, MnETR2, MnEIN3, and MnEIL1 have higher levels of expression. On the contrary, the expression of MnACS3, MnACS4, MnACO4, MnERS1, MnEIN4, MnEIL3, and MnEIL4 was relatively repressed. In addition, MnACS5 exhibited tissue-specific expression in female flowers. Among ACO genes, MnACO1 and MnACO2 exhibited tissue-biased expression in fruit, although the expression levels of MnACO1 were only about one tenth of those of MnACO2. MnEIN3 and MnEIL1 showed more expression than the rest of the EIN3 family in roots and fruits.Fig. 2

Bottom Line: A total of 145 mulberry genes were identified by both homology-based and hidden Markov model (HMM) search, including 29 genes associated with ethylene biosynthesis and 116 genes in the AP2/ERF family.The differences in gene expression were also observed in different tissues.The expression of two mulberry genes in the AP2/ERF family, MaERF-B2-1 and MaERF-B2-2, was found to be associated with the response to flooding stress.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing, 400715, China.

ABSTRACT
The phytohormone ethylene is essential to plant growth and development. It plays crucial roles in responses to biotic and abiotic stress. The mulberry tree is an important crop plant in countries in which people rear silkworms for silk production. The availability of the mulberry genome has made it possible to identify mulberry genes involved in ethylene biosynthesis and signal pathways. A total of 145 mulberry genes were identified by both homology-based and hidden Markov model (HMM) search, including 29 genes associated with ethylene biosynthesis and 116 genes in the AP2/ERF family. Studies on gene structure have provided a genetic basis for understanding the functions of these genes. The differences in gene expression were also observed in different tissues. The expression of two mulberry genes in the AP2/ERF family, MaERF-B2-1 and MaERF-B2-2, was found to be associated with the response to flooding stress.

Show MeSH