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Multiple tumor marker protein chip detection system in diagnosis of pancreatic cancer.

Liu F, Du F, Chen X - World J Surg Oncol (2014)

Bottom Line: Early symptoms of pancreatic cancer are often not obvious on imaging (ultrasound, computed tomography (CT), and so on), and when patients present with weight loss, jaundice and abdominal pain and other symptoms, they are usually already in the advanced stages of pancreatic cancer.Twelve tumor markers including AFP, CEA, NSE, CA125, CA15-3, CA242, CA19-9, PSA, f-PSA, FER, β-HCG and HGH were measured by the protein biochip detection in serum in 235 pancreatic cancer patients, 230 benign pancreatic disease patients and 240 healthy people.There was significant difference in CA19-9, NSE, CEA, CA242 and CA125 by multi-tumor marker protein biochip detection among patients with cancer, benign disease and healthy people (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, People's Hospital of Weifang, Weifang 261041, Shandong Province, China. dufutian666@sina.com.

ABSTRACT

Background: The clinical stage of the disease at diagnosis often determines the prognosis and survival rate of a patient with pancreatic cancer. Early symptoms of pancreatic cancer are often not obvious on imaging (ultrasound, computed tomography (CT), and so on), and when patients present with weight loss, jaundice and abdominal pain and other symptoms, they are usually already in the advanced stages of pancreatic cancer. However, the examination of combined tumor markers might improve their sensitivity or specificity in aiding diagnosis.

Methods: Twelve tumor markers including AFP, CEA, NSE, CA125, CA15-3, CA242, CA19-9, PSA, f-PSA, FER, β-HCG and HGH were measured by the protein biochip detection in serum in 235 pancreatic cancer patients, 230 benign pancreatic disease patients and 240 healthy people.

Results: Positive detection rates of tumor markers were: CA19-9 (49.3%), CA125 (45.1%), FER (44.2%), CA242 (42.5%), CEA (38.6%), CA15-3 (36.7%), β-HCG (29.6%), AFP (24.5%), NSE (18.2%), PSA (19.5%), f-PSA (9.4%) and HGH (8.7%) respectively. There was significant difference in CA19-9, NSE, CEA, CA242 and CA125 by multi-tumor marker protein biochip detection among patients with cancer, benign disease and healthy people (P<0.05). The positive rate of 5 tumor markers was 94.9%, and this was much higher than that of any single marker.

Conclusion: The detection of CA19-9, NSE, CEA, CA242 and CA125 in the multi-tumor marker protein biochip system is helpful in the diagnosis of pancreatic cancer.

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The workflow of the chip detection system.
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Fig1: The workflow of the chip detection system.

Mentions: One point five microliters of each of the 12 capture antibodies, with an average concentration of 1 mg/mL, were arrayed in duplicates on a 1 × 1 cm nitrocellulose membrane. The array consisted of a 5 × 5 matrix with 12 pairs of antibody spots and 1 blank spot as control. After spotting, the membrane was mounted with a plastic mold and blocked with 10% BSA. Each protein chip was incubated with 100 μL of serum, rinsed and washed with TBST (0.1 mol Tris-HCl, 8.5 g NaCl, 1 mL Tween 20, pH 7.6). The chip was then incubated with 100 μL of HRP-labeled antibodies for 30 minutes at 37°C and again rinsed and washed with TBST. Chemiluminescence substrates were added for 1 minute. Light signals were captured with the self-built chip reader based on a CCD camera and controlled with a computer system using self-developed software. The workflow of the chip detection system is shown in Figure 1.Figure 1


Multiple tumor marker protein chip detection system in diagnosis of pancreatic cancer.

Liu F, Du F, Chen X - World J Surg Oncol (2014)

The workflow of the chip detection system.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4233091&req=5

Fig1: The workflow of the chip detection system.
Mentions: One point five microliters of each of the 12 capture antibodies, with an average concentration of 1 mg/mL, were arrayed in duplicates on a 1 × 1 cm nitrocellulose membrane. The array consisted of a 5 × 5 matrix with 12 pairs of antibody spots and 1 blank spot as control. After spotting, the membrane was mounted with a plastic mold and blocked with 10% BSA. Each protein chip was incubated with 100 μL of serum, rinsed and washed with TBST (0.1 mol Tris-HCl, 8.5 g NaCl, 1 mL Tween 20, pH 7.6). The chip was then incubated with 100 μL of HRP-labeled antibodies for 30 minutes at 37°C and again rinsed and washed with TBST. Chemiluminescence substrates were added for 1 minute. Light signals were captured with the self-built chip reader based on a CCD camera and controlled with a computer system using self-developed software. The workflow of the chip detection system is shown in Figure 1.Figure 1

Bottom Line: Early symptoms of pancreatic cancer are often not obvious on imaging (ultrasound, computed tomography (CT), and so on), and when patients present with weight loss, jaundice and abdominal pain and other symptoms, they are usually already in the advanced stages of pancreatic cancer.Twelve tumor markers including AFP, CEA, NSE, CA125, CA15-3, CA242, CA19-9, PSA, f-PSA, FER, β-HCG and HGH were measured by the protein biochip detection in serum in 235 pancreatic cancer patients, 230 benign pancreatic disease patients and 240 healthy people.There was significant difference in CA19-9, NSE, CEA, CA242 and CA125 by multi-tumor marker protein biochip detection among patients with cancer, benign disease and healthy people (P<0.05).

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, People's Hospital of Weifang, Weifang 261041, Shandong Province, China. dufutian666@sina.com.

ABSTRACT

Background: The clinical stage of the disease at diagnosis often determines the prognosis and survival rate of a patient with pancreatic cancer. Early symptoms of pancreatic cancer are often not obvious on imaging (ultrasound, computed tomography (CT), and so on), and when patients present with weight loss, jaundice and abdominal pain and other symptoms, they are usually already in the advanced stages of pancreatic cancer. However, the examination of combined tumor markers might improve their sensitivity or specificity in aiding diagnosis.

Methods: Twelve tumor markers including AFP, CEA, NSE, CA125, CA15-3, CA242, CA19-9, PSA, f-PSA, FER, β-HCG and HGH were measured by the protein biochip detection in serum in 235 pancreatic cancer patients, 230 benign pancreatic disease patients and 240 healthy people.

Results: Positive detection rates of tumor markers were: CA19-9 (49.3%), CA125 (45.1%), FER (44.2%), CA242 (42.5%), CEA (38.6%), CA15-3 (36.7%), β-HCG (29.6%), AFP (24.5%), NSE (18.2%), PSA (19.5%), f-PSA (9.4%) and HGH (8.7%) respectively. There was significant difference in CA19-9, NSE, CEA, CA242 and CA125 by multi-tumor marker protein biochip detection among patients with cancer, benign disease and healthy people (P<0.05). The positive rate of 5 tumor markers was 94.9%, and this was much higher than that of any single marker.

Conclusion: The detection of CA19-9, NSE, CEA, CA242 and CA125 in the multi-tumor marker protein biochip system is helpful in the diagnosis of pancreatic cancer.

Show MeSH
Related in: MedlinePlus