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Earthworm (Pheretima aspergillum) extract stimulates osteoblast activity and inhibits osteoclast differentiation.

Fu YT, Chen KY, Chen YS, Yao CH - BMC Complement Altern Med (2014)

Bottom Line: The potential benefits of earthworm (Pheretima aspergillum) for healing have received considerable attention recently.The most effective concentration of earthworm extract was 3 mg/ml, significantly increasing osteoblast proliferation and differentiation, matrix calcium deposition and the expression levels of alkaline phosphatase, osteopontin and osteocalcin.Conversely, 3 mg/ml earthworm extract significantly reduced the tartrate-resistant acid phosphatase activity of osteoclasts without altering cell viability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan. chyao@mail.cmu.edu.tw.

ABSTRACT

Background: The potential benefits of earthworm (Pheretima aspergillum) for healing have received considerable attention recently. Osteoblast and osteoclast activities are very important in bone remodeling, which is crucial to repair bone injuries. This study investigated the effects of earthworm extract on bone cell activities.

Methods: Osteoblast-like MG-63 cells and RAW 264.7 macrophage cells were used for identifying the cellular effects of different concentrations of earthworm extract on osteoblasts and osteoclasts, respectively. The optimal concentration of earthworm extract was determined by mitochondrial colorimetric assay, alkaline phosphatase activity, matrix calcium deposition, Western blotting and tartrate-resistant acid phosphatase activity.

Results: Earthworm extract had a dose-dependent effect on bone cell activities. The most effective concentration of earthworm extract was 3 mg/ml, significantly increasing osteoblast proliferation and differentiation, matrix calcium deposition and the expression levels of alkaline phosphatase, osteopontin and osteocalcin. Conversely, 3 mg/ml earthworm extract significantly reduced the tartrate-resistant acid phosphatase activity of osteoclasts without altering cell viability.

Conclusions: Earthworm extract has beneficial effects on bone cell cultures, indicating that earthworm extract is a potential agent for use in bone regeneration.

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Related in: MedlinePlus

Earthworm extract inhibits the differentiation of osteoclasts. RAW 264.7 cells were seeded in 48-well plates and allowed to adhere for 1 day. Osteoclast differentiation from RAW 264.7 cells was induced with 50 ng/ml RANKL in α-MEM for 6 days. Osteoclasts were then cultured with PBS as a control or different concentrations of earthworm extract for 2 days. Osteoclast differentiation was determined by TRAP activity assay. Results are expressed as percentage of control (*p < 0.05 and **p < 0.01 vs. control).
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Fig7: Earthworm extract inhibits the differentiation of osteoclasts. RAW 264.7 cells were seeded in 48-well plates and allowed to adhere for 1 day. Osteoclast differentiation from RAW 264.7 cells was induced with 50 ng/ml RANKL in α-MEM for 6 days. Osteoclasts were then cultured with PBS as a control or different concentrations of earthworm extract for 2 days. Osteoclast differentiation was determined by TRAP activity assay. Results are expressed as percentage of control (*p < 0.05 and **p < 0.01 vs. control).

Mentions: The effect of earthworm extract on osteoclast differentiation was evaluated by TRAP, a marker of osteoclast differentiation[28]. Figure 7 shows TRAP activity assay results for osteoclasts cultured with various concentrations of earthworm extract for 2 days. A dose-dependent response for TRAP activity existed. Earthworm extract at concentrations < 3 mg/ml markedly decreased the TRAP activity of osteoclasts as compared with that of the control group (p < 0.05). Inhibition of TRAP activity was stronger when the earthworm extract concentration was < 500 μg/ml (p < 0.05). However, 6 mg/ml earthworm extract had no significant effect on TRAP activity (p > 0.05).Figure 7


Earthworm (Pheretima aspergillum) extract stimulates osteoblast activity and inhibits osteoclast differentiation.

Fu YT, Chen KY, Chen YS, Yao CH - BMC Complement Altern Med (2014)

Earthworm extract inhibits the differentiation of osteoclasts. RAW 264.7 cells were seeded in 48-well plates and allowed to adhere for 1 day. Osteoclast differentiation from RAW 264.7 cells was induced with 50 ng/ml RANKL in α-MEM for 6 days. Osteoclasts were then cultured with PBS as a control or different concentrations of earthworm extract for 2 days. Osteoclast differentiation was determined by TRAP activity assay. Results are expressed as percentage of control (*p < 0.05 and **p < 0.01 vs. control).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4233063&req=5

Fig7: Earthworm extract inhibits the differentiation of osteoclasts. RAW 264.7 cells were seeded in 48-well plates and allowed to adhere for 1 day. Osteoclast differentiation from RAW 264.7 cells was induced with 50 ng/ml RANKL in α-MEM for 6 days. Osteoclasts were then cultured with PBS as a control or different concentrations of earthworm extract for 2 days. Osteoclast differentiation was determined by TRAP activity assay. Results are expressed as percentage of control (*p < 0.05 and **p < 0.01 vs. control).
Mentions: The effect of earthworm extract on osteoclast differentiation was evaluated by TRAP, a marker of osteoclast differentiation[28]. Figure 7 shows TRAP activity assay results for osteoclasts cultured with various concentrations of earthworm extract for 2 days. A dose-dependent response for TRAP activity existed. Earthworm extract at concentrations < 3 mg/ml markedly decreased the TRAP activity of osteoclasts as compared with that of the control group (p < 0.05). Inhibition of TRAP activity was stronger when the earthworm extract concentration was < 500 μg/ml (p < 0.05). However, 6 mg/ml earthworm extract had no significant effect on TRAP activity (p > 0.05).Figure 7

Bottom Line: The potential benefits of earthworm (Pheretima aspergillum) for healing have received considerable attention recently.The most effective concentration of earthworm extract was 3 mg/ml, significantly increasing osteoblast proliferation and differentiation, matrix calcium deposition and the expression levels of alkaline phosphatase, osteopontin and osteocalcin.Conversely, 3 mg/ml earthworm extract significantly reduced the tartrate-resistant acid phosphatase activity of osteoclasts without altering cell viability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan. chyao@mail.cmu.edu.tw.

ABSTRACT

Background: The potential benefits of earthworm (Pheretima aspergillum) for healing have received considerable attention recently. Osteoblast and osteoclast activities are very important in bone remodeling, which is crucial to repair bone injuries. This study investigated the effects of earthworm extract on bone cell activities.

Methods: Osteoblast-like MG-63 cells and RAW 264.7 macrophage cells were used for identifying the cellular effects of different concentrations of earthworm extract on osteoblasts and osteoclasts, respectively. The optimal concentration of earthworm extract was determined by mitochondrial colorimetric assay, alkaline phosphatase activity, matrix calcium deposition, Western blotting and tartrate-resistant acid phosphatase activity.

Results: Earthworm extract had a dose-dependent effect on bone cell activities. The most effective concentration of earthworm extract was 3 mg/ml, significantly increasing osteoblast proliferation and differentiation, matrix calcium deposition and the expression levels of alkaline phosphatase, osteopontin and osteocalcin. Conversely, 3 mg/ml earthworm extract significantly reduced the tartrate-resistant acid phosphatase activity of osteoclasts without altering cell viability.

Conclusions: Earthworm extract has beneficial effects on bone cell cultures, indicating that earthworm extract is a potential agent for use in bone regeneration.

Show MeSH
Related in: MedlinePlus