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Earthworm (Pheretima aspergillum) extract stimulates osteoblast activity and inhibits osteoclast differentiation.

Fu YT, Chen KY, Chen YS, Yao CH - BMC Complement Altern Med (2014)

Bottom Line: The potential benefits of earthworm (Pheretima aspergillum) for healing have received considerable attention recently.The most effective concentration of earthworm extract was 3 mg/ml, significantly increasing osteoblast proliferation and differentiation, matrix calcium deposition and the expression levels of alkaline phosphatase, osteopontin and osteocalcin.Conversely, 3 mg/ml earthworm extract significantly reduced the tartrate-resistant acid phosphatase activity of osteoclasts without altering cell viability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan. chyao@mail.cmu.edu.tw.

ABSTRACT

Background: The potential benefits of earthworm (Pheretima aspergillum) for healing have received considerable attention recently. Osteoblast and osteoclast activities are very important in bone remodeling, which is crucial to repair bone injuries. This study investigated the effects of earthworm extract on bone cell activities.

Methods: Osteoblast-like MG-63 cells and RAW 264.7 macrophage cells were used for identifying the cellular effects of different concentrations of earthworm extract on osteoblasts and osteoclasts, respectively. The optimal concentration of earthworm extract was determined by mitochondrial colorimetric assay, alkaline phosphatase activity, matrix calcium deposition, Western blotting and tartrate-resistant acid phosphatase activity.

Results: Earthworm extract had a dose-dependent effect on bone cell activities. The most effective concentration of earthworm extract was 3 mg/ml, significantly increasing osteoblast proliferation and differentiation, matrix calcium deposition and the expression levels of alkaline phosphatase, osteopontin and osteocalcin. Conversely, 3 mg/ml earthworm extract significantly reduced the tartrate-resistant acid phosphatase activity of osteoclasts without altering cell viability.

Conclusions: Earthworm extract has beneficial effects on bone cell cultures, indicating that earthworm extract is a potential agent for use in bone regeneration.

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Related in: MedlinePlus

Earthworm extract stimulates matrix calcium deposition by osteoblasts. MG-63 cells were cultured in osteogenic medium mixed with PBS as a control or different concentrations of earthworm extract. After 14 day of culture, matrix calcium deposition was determined using von Kossa staining. Arrows demonstrate deposition of mineralized matrix.
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Fig4: Earthworm extract stimulates matrix calcium deposition by osteoblasts. MG-63 cells were cultured in osteogenic medium mixed with PBS as a control or different concentrations of earthworm extract. After 14 day of culture, matrix calcium deposition was determined using von Kossa staining. Arrows demonstrate deposition of mineralized matrix.

Mentions: Von Kossa staining is specific for the calcified extracellular matrix[27]. Dark staining of nodules demonstrates deposition of the mineralized matrix of bone formation. Figure 4 shows the effect of various concentrations of earthworm extract on matrix calcium deposition after 14 days of incubation. Osteoblasts cultured in medium containing 500 μg/ml to 3 mg/ml earthworm extract had high matrix mineralization. However, mineralization of osteoblasts treated with 12 mg/ml earthworm extract was not observed after culturing for 14 days.Figure 4


Earthworm (Pheretima aspergillum) extract stimulates osteoblast activity and inhibits osteoclast differentiation.

Fu YT, Chen KY, Chen YS, Yao CH - BMC Complement Altern Med (2014)

Earthworm extract stimulates matrix calcium deposition by osteoblasts. MG-63 cells were cultured in osteogenic medium mixed with PBS as a control or different concentrations of earthworm extract. After 14 day of culture, matrix calcium deposition was determined using von Kossa staining. Arrows demonstrate deposition of mineralized matrix.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4233063&req=5

Fig4: Earthworm extract stimulates matrix calcium deposition by osteoblasts. MG-63 cells were cultured in osteogenic medium mixed with PBS as a control or different concentrations of earthworm extract. After 14 day of culture, matrix calcium deposition was determined using von Kossa staining. Arrows demonstrate deposition of mineralized matrix.
Mentions: Von Kossa staining is specific for the calcified extracellular matrix[27]. Dark staining of nodules demonstrates deposition of the mineralized matrix of bone formation. Figure 4 shows the effect of various concentrations of earthworm extract on matrix calcium deposition after 14 days of incubation. Osteoblasts cultured in medium containing 500 μg/ml to 3 mg/ml earthworm extract had high matrix mineralization. However, mineralization of osteoblasts treated with 12 mg/ml earthworm extract was not observed after culturing for 14 days.Figure 4

Bottom Line: The potential benefits of earthworm (Pheretima aspergillum) for healing have received considerable attention recently.The most effective concentration of earthworm extract was 3 mg/ml, significantly increasing osteoblast proliferation and differentiation, matrix calcium deposition and the expression levels of alkaline phosphatase, osteopontin and osteocalcin.Conversely, 3 mg/ml earthworm extract significantly reduced the tartrate-resistant acid phosphatase activity of osteoclasts without altering cell viability.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan. chyao@mail.cmu.edu.tw.

ABSTRACT

Background: The potential benefits of earthworm (Pheretima aspergillum) for healing have received considerable attention recently. Osteoblast and osteoclast activities are very important in bone remodeling, which is crucial to repair bone injuries. This study investigated the effects of earthworm extract on bone cell activities.

Methods: Osteoblast-like MG-63 cells and RAW 264.7 macrophage cells were used for identifying the cellular effects of different concentrations of earthworm extract on osteoblasts and osteoclasts, respectively. The optimal concentration of earthworm extract was determined by mitochondrial colorimetric assay, alkaline phosphatase activity, matrix calcium deposition, Western blotting and tartrate-resistant acid phosphatase activity.

Results: Earthworm extract had a dose-dependent effect on bone cell activities. The most effective concentration of earthworm extract was 3 mg/ml, significantly increasing osteoblast proliferation and differentiation, matrix calcium deposition and the expression levels of alkaline phosphatase, osteopontin and osteocalcin. Conversely, 3 mg/ml earthworm extract significantly reduced the tartrate-resistant acid phosphatase activity of osteoclasts without altering cell viability.

Conclusions: Earthworm extract has beneficial effects on bone cell cultures, indicating that earthworm extract is a potential agent for use in bone regeneration.

Show MeSH
Related in: MedlinePlus