Mixed-polarization phenotype of ascites-associated macrophages in human ovarian carcinoma: correlation of CD163 expression, cytokine levels and early relapse.
Bottom Line: However, global gene expression profiles determined for 17 of these patients revealed a mixed-polarization phenotype unrelated to the M1/M2 classification.CD163 surface expression also correlated with the ascites levels of IL-6 and IL-10 (p < 0.05), both cytokines induced CD163 expression, and their ascites levels showed a clear inverse association with RFS (p < 0.01).These findings define a subgroup of patients with high CD163 expression, high IL-6 and/or IL-10 levels and poor clinical outcome.
Affiliation: Clinic for Gynecology, Gynecological Oncology and Gynecological Endocrinology, Philipps University, Marburg, Germany.Show MeSH
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Mentions: In view of the observed correlations of RFS, CD163 surface expression and STAT3-activating cytokines (Fig. 6), we searched for associations of the TAM transcriptomes with STAT signaling. Kang et al. recently published a meta-analysis of STAT-binding sites identified by ChIP-Seq analyses.20 We used a 65-gene “STAT signature” derived from our study for hierarchal clustering of the 17 TAM samples analyzed by expression profiling. As shown by the heat map in Supporting Information Figure S3, the resulting rank order did not show any significant association with CD163 surface expression or IL-6 or IL-10 ascites levels, suggesting that elevated CD163 expression or high cytokine levels are not indicative of an activated STAT3 pathway.
Affiliation: Clinic for Gynecology, Gynecological Oncology and Gynecological Endocrinology, Philipps University, Marburg, Germany.