Limits...
Mixed-polarization phenotype of ascites-associated macrophages in human ovarian carcinoma: correlation of CD163 expression, cytokine levels and early relapse.

Reinartz S, Schumann T, Finkernagel F, Wortmann A, Jansen JM, Meissner W, Krause M, Schwörer AM, Wagner U, Müller-Brüsselbach S, Müller R - Int. J. Cancer (2013)

Bottom Line: However, global gene expression profiles determined for 17 of these patients revealed a mixed-polarization phenotype unrelated to the M1/M2 classification.CD163 surface expression also correlated with the ascites levels of IL-6 and IL-10 (p < 0.05), both cytokines induced CD163 expression, and their ascites levels showed a clear inverse association with RFS (p < 0.01).These findings define a subgroup of patients with high CD163 expression, high IL-6 and/or IL-10 levels and poor clinical outcome.

View Article: PubMed Central - PubMed

Affiliation: Clinic for Gynecology, Gynecological Oncology and Gynecological Endocrinology, Philipps University, Marburg, Germany.

Show MeSH

Related in: MedlinePlus

Summary of the correlations identified in our study. Patients were categorized according to the levels of CD163, IL-6 and IL-10 (high in panel a and low in panel b). The bottom row shows the RFS times after first-line surgery. Empty field represent patients that are not yet evaluable (relapse-free <12 months postsurgery). Panel c shows a schematic representations of the correlations between CD163, IL-6, IL-10 and RFS. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4232932&req=5

fig06: Summary of the correlations identified in our study. Patients were categorized according to the levels of CD163, IL-6 and IL-10 (high in panel a and low in panel b). The bottom row shows the RFS times after first-line surgery. Empty field represent patients that are not yet evaluable (relapse-free <12 months postsurgery). Panel c shows a schematic representations of the correlations between CD163, IL-6, IL-10 and RFS. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Mentions: In view of the observed correlations of RFS, CD163 surface expression and STAT3-activating cytokines (Fig. 6), we searched for associations of the TAM transcriptomes with STAT signaling. Kang et al. recently published a meta-analysis of STAT-binding sites identified by ChIP-Seq analyses.20 We used a 65-gene “STAT signature” derived from our study for hierarchal clustering of the 17 TAM samples analyzed by expression profiling. As shown by the heat map in Supporting Information Figure S3, the resulting rank order did not show any significant association with CD163 surface expression or IL-6 or IL-10 ascites levels, suggesting that elevated CD163 expression or high cytokine levels are not indicative of an activated STAT3 pathway.


Mixed-polarization phenotype of ascites-associated macrophages in human ovarian carcinoma: correlation of CD163 expression, cytokine levels and early relapse.

Reinartz S, Schumann T, Finkernagel F, Wortmann A, Jansen JM, Meissner W, Krause M, Schwörer AM, Wagner U, Müller-Brüsselbach S, Müller R - Int. J. Cancer (2013)

Summary of the correlations identified in our study. Patients were categorized according to the levels of CD163, IL-6 and IL-10 (high in panel a and low in panel b). The bottom row shows the RFS times after first-line surgery. Empty field represent patients that are not yet evaluable (relapse-free <12 months postsurgery). Panel c shows a schematic representations of the correlations between CD163, IL-6, IL-10 and RFS. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232932&req=5

fig06: Summary of the correlations identified in our study. Patients were categorized according to the levels of CD163, IL-6 and IL-10 (high in panel a and low in panel b). The bottom row shows the RFS times after first-line surgery. Empty field represent patients that are not yet evaluable (relapse-free <12 months postsurgery). Panel c shows a schematic representations of the correlations between CD163, IL-6, IL-10 and RFS. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
Mentions: In view of the observed correlations of RFS, CD163 surface expression and STAT3-activating cytokines (Fig. 6), we searched for associations of the TAM transcriptomes with STAT signaling. Kang et al. recently published a meta-analysis of STAT-binding sites identified by ChIP-Seq analyses.20 We used a 65-gene “STAT signature” derived from our study for hierarchal clustering of the 17 TAM samples analyzed by expression profiling. As shown by the heat map in Supporting Information Figure S3, the resulting rank order did not show any significant association with CD163 surface expression or IL-6 or IL-10 ascites levels, suggesting that elevated CD163 expression or high cytokine levels are not indicative of an activated STAT3 pathway.

Bottom Line: However, global gene expression profiles determined for 17 of these patients revealed a mixed-polarization phenotype unrelated to the M1/M2 classification.CD163 surface expression also correlated with the ascites levels of IL-6 and IL-10 (p < 0.05), both cytokines induced CD163 expression, and their ascites levels showed a clear inverse association with RFS (p < 0.01).These findings define a subgroup of patients with high CD163 expression, high IL-6 and/or IL-10 levels and poor clinical outcome.

View Article: PubMed Central - PubMed

Affiliation: Clinic for Gynecology, Gynecological Oncology and Gynecological Endocrinology, Philipps University, Marburg, Germany.

Show MeSH
Related in: MedlinePlus