Mixed-polarization phenotype of ascites-associated macrophages in human ovarian carcinoma: correlation of CD163 expression, cytokine levels and early relapse.
Bottom Line: However, global gene expression profiles determined for 17 of these patients revealed a mixed-polarization phenotype unrelated to the M1/M2 classification.CD163 surface expression also correlated with the ascites levels of IL-6 and IL-10 (p < 0.05), both cytokines induced CD163 expression, and their ascites levels showed a clear inverse association with RFS (p < 0.01).These findings define a subgroup of patients with high CD163 expression, high IL-6 and/or IL-10 levels and poor clinical outcome.
Affiliation: Clinic for Gynecology, Gynecological Oncology and Gynecological Endocrinology, Philipps University, Marburg, Germany.Show MeSH
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Mentions: The association of CD163 surface expression with cytokine levels (see above) prompted us to investigate potential associations between RFS and the concentrations of cytokines in the ascites fluid from the same patients. Patients were grouped as “high” or “low” for each cytokine (cutoff 1,500 ng/ml for IL-6; 200 ng/ml for IL-10 and LIF; Supporting Information Dataset S1). A clear inverse association was observed for IL-6 (log-rank test, p = 0.0015; Fig. 5a), IL-10 (p = 0.0033; Fig. 5b) and LIF (p = 0.0183; Fig. 5c), all three of which are STAT3-inducing cytokines. In contrast, no association was detectable between RFS and the ascites level of leptin, CSF-1 and CCL2, TGF-β or ANGPTL4 (Figs. 5d and 5f) or VEGF-A, VEGF-C and CA125 (graphs not shown; all p-values > 0.3).
Affiliation: Clinic for Gynecology, Gynecological Oncology and Gynecological Endocrinology, Philipps University, Marburg, Germany.