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Mixed-polarization phenotype of ascites-associated macrophages in human ovarian carcinoma: correlation of CD163 expression, cytokine levels and early relapse.

Reinartz S, Schumann T, Finkernagel F, Wortmann A, Jansen JM, Meissner W, Krause M, Schwörer AM, Wagner U, Müller-Brüsselbach S, Müller R - Int. J. Cancer (2013)

Bottom Line: However, global gene expression profiles determined for 17 of these patients revealed a mixed-polarization phenotype unrelated to the M1/M2 classification.CD163 surface expression also correlated with the ascites levels of IL-6 and IL-10 (p < 0.05), both cytokines induced CD163 expression, and their ascites levels showed a clear inverse association with RFS (p < 0.01).These findings define a subgroup of patients with high CD163 expression, high IL-6 and/or IL-10 levels and poor clinical outcome.

View Article: PubMed Central - PubMed

Affiliation: Clinic for Gynecology, Gynecological Oncology and Gynecological Endocrinology, Philipps University, Marburg, Germany.

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CD163 surface expression is associated with IL-6 and IL-10. (a and b) Correlation of CD163 surface expression with ascites levels of IL-6 and IL-10. *p < 0.05; **p < 0.01 by Pearson’s r correlation. (c) Induction of CD163 in TAMs and MDMs by ascites and/or IL-10 and IL-10. TAMs were cultured for 2 days with or without cell-free ascites (10% of total culture medium). MDMs from healthy donors were differentiated in the presence of GM-CSF and either cell-free ascites (10% of total culture medium) or the indicated recombinant cytokines for 5 days. Expression of surface marker CD163 was determined by FACS and is expressed as fold induction, i.e., normalized to 1 for the respective untreated control (untreated TAMs and MMDs, respectively, are represented by the dotted line). Each data point within the same treatment group represents a different donor. Horizontal bars indicate averages. The induction value represented by the open box could not be precisely determined because of an extremely low expression value in untreated MDMs. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
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fig04: CD163 surface expression is associated with IL-6 and IL-10. (a and b) Correlation of CD163 surface expression with ascites levels of IL-6 and IL-10. *p < 0.05; **p < 0.01 by Pearson’s r correlation. (c) Induction of CD163 in TAMs and MDMs by ascites and/or IL-10 and IL-10. TAMs were cultured for 2 days with or without cell-free ascites (10% of total culture medium). MDMs from healthy donors were differentiated in the presence of GM-CSF and either cell-free ascites (10% of total culture medium) or the indicated recombinant cytokines for 5 days. Expression of surface marker CD163 was determined by FACS and is expressed as fold induction, i.e., normalized to 1 for the respective untreated control (untreated TAMs and MMDs, respectively, are represented by the dotted line). Each data point within the same treatment group represents a different donor. Horizontal bars indicate averages. The induction value represented by the open box could not be precisely determined because of an extremely low expression value in untreated MDMs. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Mentions: The data described above raised the possibility that the expression of CD163 on TAMs and the ascites levels of cytokines might be linked. Therefore, we tested this hypothesis for pairs of the respective datasets (i.e., CD163 and cytokine values for the same patient) using Pearson’s r (Supporting Information Table S2). This analysis indeed confirmed a significant correlation of CD163 expression with the concentration of both IL-6 (p = 0.0483; correlation coefficient r = 0.1471; Fig. 4a) and IL-10 (p = 0.0459; r = 0.1501; Fig. 4b). None of the other cytokines tested was significantly associated with CD163 expression (Supporting Information Table S2).


Mixed-polarization phenotype of ascites-associated macrophages in human ovarian carcinoma: correlation of CD163 expression, cytokine levels and early relapse.

Reinartz S, Schumann T, Finkernagel F, Wortmann A, Jansen JM, Meissner W, Krause M, Schwörer AM, Wagner U, Müller-Brüsselbach S, Müller R - Int. J. Cancer (2013)

CD163 surface expression is associated with IL-6 and IL-10. (a and b) Correlation of CD163 surface expression with ascites levels of IL-6 and IL-10. *p < 0.05; **p < 0.01 by Pearson’s r correlation. (c) Induction of CD163 in TAMs and MDMs by ascites and/or IL-10 and IL-10. TAMs were cultured for 2 days with or without cell-free ascites (10% of total culture medium). MDMs from healthy donors were differentiated in the presence of GM-CSF and either cell-free ascites (10% of total culture medium) or the indicated recombinant cytokines for 5 days. Expression of surface marker CD163 was determined by FACS and is expressed as fold induction, i.e., normalized to 1 for the respective untreated control (untreated TAMs and MMDs, respectively, are represented by the dotted line). Each data point within the same treatment group represents a different donor. Horizontal bars indicate averages. The induction value represented by the open box could not be precisely determined because of an extremely low expression value in untreated MDMs. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232932&req=5

fig04: CD163 surface expression is associated with IL-6 and IL-10. (a and b) Correlation of CD163 surface expression with ascites levels of IL-6 and IL-10. *p < 0.05; **p < 0.01 by Pearson’s r correlation. (c) Induction of CD163 in TAMs and MDMs by ascites and/or IL-10 and IL-10. TAMs were cultured for 2 days with or without cell-free ascites (10% of total culture medium). MDMs from healthy donors were differentiated in the presence of GM-CSF and either cell-free ascites (10% of total culture medium) or the indicated recombinant cytokines for 5 days. Expression of surface marker CD163 was determined by FACS and is expressed as fold induction, i.e., normalized to 1 for the respective untreated control (untreated TAMs and MMDs, respectively, are represented by the dotted line). Each data point within the same treatment group represents a different donor. Horizontal bars indicate averages. The induction value represented by the open box could not be precisely determined because of an extremely low expression value in untreated MDMs. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
Mentions: The data described above raised the possibility that the expression of CD163 on TAMs and the ascites levels of cytokines might be linked. Therefore, we tested this hypothesis for pairs of the respective datasets (i.e., CD163 and cytokine values for the same patient) using Pearson’s r (Supporting Information Table S2). This analysis indeed confirmed a significant correlation of CD163 expression with the concentration of both IL-6 (p = 0.0483; correlation coefficient r = 0.1471; Fig. 4a) and IL-10 (p = 0.0459; r = 0.1501; Fig. 4b). None of the other cytokines tested was significantly associated with CD163 expression (Supporting Information Table S2).

Bottom Line: However, global gene expression profiles determined for 17 of these patients revealed a mixed-polarization phenotype unrelated to the M1/M2 classification.CD163 surface expression also correlated with the ascites levels of IL-6 and IL-10 (p < 0.05), both cytokines induced CD163 expression, and their ascites levels showed a clear inverse association with RFS (p < 0.01).These findings define a subgroup of patients with high CD163 expression, high IL-6 and/or IL-10 levels and poor clinical outcome.

View Article: PubMed Central - PubMed

Affiliation: Clinic for Gynecology, Gynecological Oncology and Gynecological Endocrinology, Philipps University, Marburg, Germany.

Show MeSH
Related in: MedlinePlus