Double staining with crystal violet and methylene blue is appropriate for colonic endocytoscopy: an in vivo prospective pilot study.
Bottom Line: Time for each parameter to become 'recognizable' was measured, and the average times for the three staining regimens were compared.MB alone and CM double staining resulted in 'recognizable' (102 ± 27 vs 89 ± 22 s, P=0.263) nuclei within comparable periods of time, whereas CV alone was unable to identify nuclei.Double staining with CV and MB, which rapidly provided recognizable images of both nuclei and gland formation, is an appropriate staining regimen for colonic EC.
Affiliation: Digestive Disease Center, Showa University Northern Yokohama Hospital, Yokohama, Japan.Show MeSH
Mentions: Figure 2 shows the patient flowchart. From May 2011 to March 2012, 30 patients (24 males and six females, mean age 65 ± 11 years) were allocated equally to the three groups as follows. Ten (eight males, two females, mean age 65 ± 12 years) were assessed with CV alone, 10 (seven males, three females, mean age 61 ± 9 years) were assessed with MB alone, and 10 (nine males, one female, mean age 70 ± 10 years) were assessed with CM double. EC images were obtained for all patients. None experienced EC complications or adverse reactions.
Affiliation: Digestive Disease Center, Showa University Northern Yokohama Hospital, Yokohama, Japan.