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IKVAV regulates ERK1/2 and Akt signalling pathways in BMMSC population growth and proliferation.

Li B, Qiu T, Zhang P, Wang X, Yin Y, Li S - Cell Prolif. (2014)

Bottom Line: IKVAV peptides were synthesized by the solid-phase method.Meanwhile, phosphorylation levels of ERK1/2 and Akt were partially blocked by ERK1/2 inhibitor (PD98059) and Akt inhibitor (wortmannin), respectively.This study is the first to reveal an enhancement effect of IKVAV peptide on BMMSC at the signal transduction level, and the outcome could provide experimental evidence for application of IKVAV-grafted scaffolds in the field of BMMSC-based tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, and Biomaterials Science and Engineering Research Center, Wuhan University of Technology, Wuhan, 430070, China.

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Effects of two inhibitors on PCNA synthesis of IKVAV-induced BMMSCs. BMMSCs were pretreated by PD98059 (10 μm) or wortmannin (100 nm) or both of them, then stimulated by 0.5 mmIKVAV. The expression of PCNA was detected by RT-PCR. Experiments were performed at least in triplicate (*P < 0.05).
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fig06: Effects of two inhibitors on PCNA synthesis of IKVAV-induced BMMSCs. BMMSCs were pretreated by PD98059 (10 μm) or wortmannin (100 nm) or both of them, then stimulated by 0.5 mmIKVAV. The expression of PCNA was detected by RT-PCR. Experiments were performed at least in triplicate (*P < 0.05).

Mentions: RT-PCR was used to test mRNA synthesis of PCNA in BMMSC pre-treated with the inhibitors. Results in Fig.6 show that PCNA expression was reduced to 27.14% by PD98059 compared to the untreated group, to 51.49% by wortmannin, and to 77.99% by simultaneous use of both inhibitors. Furthermore, expressions of PCNA mRNA were significantly different when comparing IKVAV-induced BMMSCs with the control group (*P < 0.05), PD98059-treated group (*P < 0.05) and the wortmannin-treated group (*P < 0.05).


IKVAV regulates ERK1/2 and Akt signalling pathways in BMMSC population growth and proliferation.

Li B, Qiu T, Zhang P, Wang X, Yin Y, Li S - Cell Prolif. (2014)

Effects of two inhibitors on PCNA synthesis of IKVAV-induced BMMSCs. BMMSCs were pretreated by PD98059 (10 μm) or wortmannin (100 nm) or both of them, then stimulated by 0.5 mmIKVAV. The expression of PCNA was detected by RT-PCR. Experiments were performed at least in triplicate (*P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232901&req=5

fig06: Effects of two inhibitors on PCNA synthesis of IKVAV-induced BMMSCs. BMMSCs were pretreated by PD98059 (10 μm) or wortmannin (100 nm) or both of them, then stimulated by 0.5 mmIKVAV. The expression of PCNA was detected by RT-PCR. Experiments were performed at least in triplicate (*P < 0.05).
Mentions: RT-PCR was used to test mRNA synthesis of PCNA in BMMSC pre-treated with the inhibitors. Results in Fig.6 show that PCNA expression was reduced to 27.14% by PD98059 compared to the untreated group, to 51.49% by wortmannin, and to 77.99% by simultaneous use of both inhibitors. Furthermore, expressions of PCNA mRNA were significantly different when comparing IKVAV-induced BMMSCs with the control group (*P < 0.05), PD98059-treated group (*P < 0.05) and the wortmannin-treated group (*P < 0.05).

Bottom Line: IKVAV peptides were synthesized by the solid-phase method.Meanwhile, phosphorylation levels of ERK1/2 and Akt were partially blocked by ERK1/2 inhibitor (PD98059) and Akt inhibitor (wortmannin), respectively.This study is the first to reveal an enhancement effect of IKVAV peptide on BMMSC at the signal transduction level, and the outcome could provide experimental evidence for application of IKVAV-grafted scaffolds in the field of BMMSC-based tissue engineering.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, and Biomaterials Science and Engineering Research Center, Wuhan University of Technology, Wuhan, 430070, China.

Show MeSH
Related in: MedlinePlus