The ortholog of the human proto-oncogene ROS1 is required for epithelial development in C. elegans.
Bottom Line: Little is known about the role of ROS1, however in vertebrates it has been implicated in promoting differentiation programs in specialized epithelial tissues.We also provide evidence of a direct relationship between ROL-3, the mucin SRAP-1, and BCC-1, the homolog of mRNA regulating protein Bicaudal-C.This study answers a longstanding question as to the developmental function of ROL-3, identifies three new genes that are expressed and function in the developing epithelium of C. elegans, and introduces the nematode as a potentially powerful model system for investigating the increasingly important, yet poorly understood, human oncogene ROS1.
Affiliation: Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z4. email@example.comShow MeSH
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Mentions: To identify the tissues in which rol-3 is expressed, we generated a transcriptional reporter containing 3kb of the rol-3 5′ flanking region fused to the GFP sequence containing a NLS (rol-3::GFP). Animals carrying rol-3::GFP as a transgenic array (sEx1594) express GFP in all major hypodermal nuclei, with the exception of the seam cells (Fig. 2a–f). Expression is first detected during dorsal intercalation and is particularly intense throughout embryogenesis (Fig. 2a–d). Upon hatching rol-3::GFP expression persists in the major hypodermis at a relatively low-level throughout development up until the adult stage, where expression is not detected (Fig. 2e,f and data not shown).
Affiliation: Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z4. firstname.lastname@example.org