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The ortholog of the human proto-oncogene ROS1 is required for epithelial development in C. elegans.

Jones MR, Rose AM, Baillie DL - Genesis (2013)

Bottom Line: Little is known about the role of ROS1, however in vertebrates it has been implicated in promoting differentiation programs in specialized epithelial tissues.We also provide evidence of a direct relationship between ROL-3, the mucin SRAP-1, and BCC-1, the homolog of mRNA regulating protein Bicaudal-C.This study answers a longstanding question as to the developmental function of ROL-3, identifies three new genes that are expressed and function in the developing epithelium of C. elegans, and introduces the nematode as a potentially powerful model system for investigating the increasingly important, yet poorly understood, human oncogene ROS1.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z4. jonesmr@mail.ubc.ca

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ROL-3 is expressed in a dynamic pattern in the developing hypodermis. a–f, A rol-3::GFP transcriptional fusion is expressed in the major hypodermis throughout development. Expression begins at the embryonic stage in most, if not all, major hypodermal cells (arrowheads a–d) and continues throughout post-embryonic development (arrowheads e and f). Expression is not observed in the seam cells (arrows a–f). g–k, A dynamic pattern of ROL-3::GFP accumulation is observed throughout post-embryonic development. Broad expression of ROL-3::GFP is seen in the major hypodermis and accumulates in foci adjacent to seam cell boundaries (arrowheads g–i). ROL-3::GFP does not localize with the apical junctions between seam cells (arrows g–i). Prior to seam cell division broad expression of ROL-3::GFP is present in the hypoderm (arrowhead j), but is not present in foci at the seam cell boundary (arrow j). As seam cells divide asymmetrically ROL-3::GFP accumulates at the cell boundaries (arrow k). Localization at anterior daughters is lost as they migrate and fuse with the hypodermis (asterisk k). Localization at cell boundaries persists as seam cell elongation is completed (arrow l) (V = Vulva). m-n, Strong expression of ROL-3::GFP is observed in VPCs at the L1 (m) and early L2 (n) stage. Scale bars, 25 μm.
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fig02: ROL-3 is expressed in a dynamic pattern in the developing hypodermis. a–f, A rol-3::GFP transcriptional fusion is expressed in the major hypodermis throughout development. Expression begins at the embryonic stage in most, if not all, major hypodermal cells (arrowheads a–d) and continues throughout post-embryonic development (arrowheads e and f). Expression is not observed in the seam cells (arrows a–f). g–k, A dynamic pattern of ROL-3::GFP accumulation is observed throughout post-embryonic development. Broad expression of ROL-3::GFP is seen in the major hypodermis and accumulates in foci adjacent to seam cell boundaries (arrowheads g–i). ROL-3::GFP does not localize with the apical junctions between seam cells (arrows g–i). Prior to seam cell division broad expression of ROL-3::GFP is present in the hypoderm (arrowhead j), but is not present in foci at the seam cell boundary (arrow j). As seam cells divide asymmetrically ROL-3::GFP accumulates at the cell boundaries (arrow k). Localization at anterior daughters is lost as they migrate and fuse with the hypodermis (asterisk k). Localization at cell boundaries persists as seam cell elongation is completed (arrow l) (V = Vulva). m-n, Strong expression of ROL-3::GFP is observed in VPCs at the L1 (m) and early L2 (n) stage. Scale bars, 25 μm.

Mentions: To identify the tissues in which rol-3 is expressed, we generated a transcriptional reporter containing 3kb of the rol-3 5′ flanking region fused to the GFP sequence containing a NLS (rol-3::GFP). Animals carrying rol-3::GFP as a transgenic array (sEx1594) express GFP in all major hypodermal nuclei, with the exception of the seam cells (Fig. 2a–f). Expression is first detected during dorsal intercalation and is particularly intense throughout embryogenesis (Fig. 2a–d). Upon hatching rol-3::GFP expression persists in the major hypodermis at a relatively low-level throughout development up until the adult stage, where expression is not detected (Fig. 2e,f and data not shown).


The ortholog of the human proto-oncogene ROS1 is required for epithelial development in C. elegans.

Jones MR, Rose AM, Baillie DL - Genesis (2013)

ROL-3 is expressed in a dynamic pattern in the developing hypodermis. a–f, A rol-3::GFP transcriptional fusion is expressed in the major hypodermis throughout development. Expression begins at the embryonic stage in most, if not all, major hypodermal cells (arrowheads a–d) and continues throughout post-embryonic development (arrowheads e and f). Expression is not observed in the seam cells (arrows a–f). g–k, A dynamic pattern of ROL-3::GFP accumulation is observed throughout post-embryonic development. Broad expression of ROL-3::GFP is seen in the major hypodermis and accumulates in foci adjacent to seam cell boundaries (arrowheads g–i). ROL-3::GFP does not localize with the apical junctions between seam cells (arrows g–i). Prior to seam cell division broad expression of ROL-3::GFP is present in the hypoderm (arrowhead j), but is not present in foci at the seam cell boundary (arrow j). As seam cells divide asymmetrically ROL-3::GFP accumulates at the cell boundaries (arrow k). Localization at anterior daughters is lost as they migrate and fuse with the hypodermis (asterisk k). Localization at cell boundaries persists as seam cell elongation is completed (arrow l) (V = Vulva). m-n, Strong expression of ROL-3::GFP is observed in VPCs at the L1 (m) and early L2 (n) stage. Scale bars, 25 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4232869&req=5

fig02: ROL-3 is expressed in a dynamic pattern in the developing hypodermis. a–f, A rol-3::GFP transcriptional fusion is expressed in the major hypodermis throughout development. Expression begins at the embryonic stage in most, if not all, major hypodermal cells (arrowheads a–d) and continues throughout post-embryonic development (arrowheads e and f). Expression is not observed in the seam cells (arrows a–f). g–k, A dynamic pattern of ROL-3::GFP accumulation is observed throughout post-embryonic development. Broad expression of ROL-3::GFP is seen in the major hypodermis and accumulates in foci adjacent to seam cell boundaries (arrowheads g–i). ROL-3::GFP does not localize with the apical junctions between seam cells (arrows g–i). Prior to seam cell division broad expression of ROL-3::GFP is present in the hypoderm (arrowhead j), but is not present in foci at the seam cell boundary (arrow j). As seam cells divide asymmetrically ROL-3::GFP accumulates at the cell boundaries (arrow k). Localization at anterior daughters is lost as they migrate and fuse with the hypodermis (asterisk k). Localization at cell boundaries persists as seam cell elongation is completed (arrow l) (V = Vulva). m-n, Strong expression of ROL-3::GFP is observed in VPCs at the L1 (m) and early L2 (n) stage. Scale bars, 25 μm.
Mentions: To identify the tissues in which rol-3 is expressed, we generated a transcriptional reporter containing 3kb of the rol-3 5′ flanking region fused to the GFP sequence containing a NLS (rol-3::GFP). Animals carrying rol-3::GFP as a transgenic array (sEx1594) express GFP in all major hypodermal nuclei, with the exception of the seam cells (Fig. 2a–f). Expression is first detected during dorsal intercalation and is particularly intense throughout embryogenesis (Fig. 2a–d). Upon hatching rol-3::GFP expression persists in the major hypodermis at a relatively low-level throughout development up until the adult stage, where expression is not detected (Fig. 2e,f and data not shown).

Bottom Line: Little is known about the role of ROS1, however in vertebrates it has been implicated in promoting differentiation programs in specialized epithelial tissues.We also provide evidence of a direct relationship between ROL-3, the mucin SRAP-1, and BCC-1, the homolog of mRNA regulating protein Bicaudal-C.This study answers a longstanding question as to the developmental function of ROL-3, identifies three new genes that are expressed and function in the developing epithelium of C. elegans, and introduces the nematode as a potentially powerful model system for investigating the increasingly important, yet poorly understood, human oncogene ROS1.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z4. jonesmr@mail.ubc.ca

Show MeSH
Related in: MedlinePlus