The ortholog of the human proto-oncogene ROS1 is required for epithelial development in C. elegans.
Bottom Line: Little is known about the role of ROS1, however in vertebrates it has been implicated in promoting differentiation programs in specialized epithelial tissues.We also provide evidence of a direct relationship between ROL-3, the mucin SRAP-1, and BCC-1, the homolog of mRNA regulating protein Bicaudal-C.This study answers a longstanding question as to the developmental function of ROL-3, identifies three new genes that are expressed and function in the developing epithelium of C. elegans, and introduces the nematode as a potentially powerful model system for investigating the increasingly important, yet poorly understood, human oncogene ROS1.
Affiliation: Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z4. firstname.lastname@example.orgShow MeSH
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Mentions: The rol-3 locus of C. elegans encodes a type I integral membrane protein related to the sevenless subfamily of tyrosine kinase insulin receptor genes (Fig. 1b). The structure of the 2481aa protein is typical of an RTK of this class with a single transmembrane domain separating the intracellular and extracellular regions of the protein. The large extracellular portion of the protein contains an N-terminal signal peptide sequence and six fibronectin type III (Fn3) domains. The intracellular region contains a single kinase domain (TyrK; Fig. 1c). BlastP analysis identifies strong similarity between ROL-3 and the human proto-oncogene and orphan receptor ROS1 (29% similarity, 17% identity), particularly within the kinase domain (60% similarity, 35% identity), indicating that ROL-3 is the likely C. elegans ortholog of this human proto-oncogene (Acquaviva et al., 2009).
Affiliation: Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z4. email@example.com