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Effect of Euphorbia hirta plant leaf extract on immunostimulant response of Aeromonas hydrophila infected Cyprinus carpio.

Pratheepa V, Sukumaran N - PeerJ (2014)

Bottom Line: It was observed that fish have survival percentage significantly at higher concentration (50 g) of Euphorbia hirta, when compared with the control.The obtained results are statistically significant at P < 0.05 and P < 0.01 levels.This research work suggests that the plant Euphorbia hirta has immunostimulant activity by stimulating both specific and non-specific immunity at higher concentrations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Aquaculture Biotechnology, Manonmaniam Sundaranar University, Sri Paramakalyani Centre for Environmental Sciences , Alwarkurchi, Tamil Nadu , India ; CIIMAR, Marine and Environmental Research Center, University of Porto , Rua dos Bragas, Porto , Portugal.

ABSTRACT
The main objective of the present study is to improve the immune power of Cyprinus carpio by using Euphorbia hirta plant leaf extract as immunostimulants. The haematological, immunological and enzymatic studies were conducted on the medicated fish infected with Aeromonas hydrophila pathogen. The results obtained from the haematological studies show that the RBC count, WBC count and haemoglobin content were increased in the infected fish at higher concentration of leaf extract. The feeds with leaf extract of Euphorbia hirta were able to stimulate the specific immune response by increasing the titre value of antibody. It was able to stimulate the antibody production only up to the 5th day, when fed with higher concentrations of (25 g and 50 g) plant leaf extract. The plant extract showed non-specific immune responses such as lysozyme activity, phagocytic ratio, NBT assay, etc. at higher concentration (50 g) and in the same concentration (50 g), the leaf extract of Euphorbia hirta significantly eliminated the pathogen in blood and kidney. It was observed that fish have survival percentage significantly at higher concentration (50 g) of Euphorbia hirta, when compared with the control. The obtained results are statistically significant at P < 0.05 and P < 0.01 levels. This research work suggests that the plant Euphorbia hirta has immunostimulant activity by stimulating both specific and non-specific immunity at higher concentrations.

No MeSH data available.


Effect of different concentrations of leaf extract of Euphorbia hirta on number of NBT positive cells in Cyprinus carpio infected with the bacterial pathogen, Aeromonas hydrophila.
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fig-1: Effect of different concentrations of leaf extract of Euphorbia hirta on number of NBT positive cells in Cyprinus carpio infected with the bacterial pathogen, Aeromonas hydrophila.

Mentions: The results obtained from the study of phagocytic activity of Euphorbia hirta on Cyprinus carpio is graphically represented in Fig. 1. From the Fig. 1, it is clear that the fish fed with higher concentrations of leaf extract of Euphorbia hirta were able to increase the phagocytic ratio and the maximum phagocytic ratio of 62.67 ± 2.05 % was noticed on the fish fed with 50 g leaf extract of Euphorbia hirta/kg feed. The peak phagocytic ratio of 73.67 ± 1.65% was found on the 15th day after infection with the feed having 50 g leaf extract. In control fish, the phagocytic ratio was increased up to 10 days after infection and in further days it was decreased.


Effect of Euphorbia hirta plant leaf extract on immunostimulant response of Aeromonas hydrophila infected Cyprinus carpio.

Pratheepa V, Sukumaran N - PeerJ (2014)

Effect of different concentrations of leaf extract of Euphorbia hirta on number of NBT positive cells in Cyprinus carpio infected with the bacterial pathogen, Aeromonas hydrophila.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232839&req=5

fig-1: Effect of different concentrations of leaf extract of Euphorbia hirta on number of NBT positive cells in Cyprinus carpio infected with the bacterial pathogen, Aeromonas hydrophila.
Mentions: The results obtained from the study of phagocytic activity of Euphorbia hirta on Cyprinus carpio is graphically represented in Fig. 1. From the Fig. 1, it is clear that the fish fed with higher concentrations of leaf extract of Euphorbia hirta were able to increase the phagocytic ratio and the maximum phagocytic ratio of 62.67 ± 2.05 % was noticed on the fish fed with 50 g leaf extract of Euphorbia hirta/kg feed. The peak phagocytic ratio of 73.67 ± 1.65% was found on the 15th day after infection with the feed having 50 g leaf extract. In control fish, the phagocytic ratio was increased up to 10 days after infection and in further days it was decreased.

Bottom Line: It was observed that fish have survival percentage significantly at higher concentration (50 g) of Euphorbia hirta, when compared with the control.The obtained results are statistically significant at P < 0.05 and P < 0.01 levels.This research work suggests that the plant Euphorbia hirta has immunostimulant activity by stimulating both specific and non-specific immunity at higher concentrations.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Aquaculture Biotechnology, Manonmaniam Sundaranar University, Sri Paramakalyani Centre for Environmental Sciences , Alwarkurchi, Tamil Nadu , India ; CIIMAR, Marine and Environmental Research Center, University of Porto , Rua dos Bragas, Porto , Portugal.

ABSTRACT
The main objective of the present study is to improve the immune power of Cyprinus carpio by using Euphorbia hirta plant leaf extract as immunostimulants. The haematological, immunological and enzymatic studies were conducted on the medicated fish infected with Aeromonas hydrophila pathogen. The results obtained from the haematological studies show that the RBC count, WBC count and haemoglobin content were increased in the infected fish at higher concentration of leaf extract. The feeds with leaf extract of Euphorbia hirta were able to stimulate the specific immune response by increasing the titre value of antibody. It was able to stimulate the antibody production only up to the 5th day, when fed with higher concentrations of (25 g and 50 g) plant leaf extract. The plant extract showed non-specific immune responses such as lysozyme activity, phagocytic ratio, NBT assay, etc. at higher concentration (50 g) and in the same concentration (50 g), the leaf extract of Euphorbia hirta significantly eliminated the pathogen in blood and kidney. It was observed that fish have survival percentage significantly at higher concentration (50 g) of Euphorbia hirta, when compared with the control. The obtained results are statistically significant at P < 0.05 and P < 0.01 levels. This research work suggests that the plant Euphorbia hirta has immunostimulant activity by stimulating both specific and non-specific immunity at higher concentrations.

No MeSH data available.