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Determination of some B Vitamins in Sour Cherry Juice Using Dispersive Liquid-liquid Microextraction Followed by High-performance Liquid Chromatography.

Parsaei P, Bahmaei M, Ghannadi A - Iran J Pharm Res (2014)

Bottom Line: The extraction efficiency of thiamine, nicotinamide and pyridoxine was influenced by factors such as: additional salt effect, the kind and volume of disperser and extraction solvents.Gise (Prunus cerasus var.This variety was identified in high altitude areas of Isfahan province after five years of study, since 2005, by Agricultural and Natural Resources Research Center of Isfahan.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Islamic Azad University, North Tehran Branch, Tehran, Iran.

ABSTRACT
Dispersive liquid-liquid microextraction method (DLLME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in sour cherry juice. This method was rapid, simple and sensitive. Separation was accomplished using a C18 column. The optimum chromatographic conditions were found to be: mobile phase consisted of 8% methanol and 92% aqueous phase (1% (V/V) acetic acid water solution); flow rate, 0.7 mL/min; detection wavelength, 260 nm and pH, 3.3. The extraction efficiency of thiamine, nicotinamide and pyridoxine was influenced by factors such as: additional salt effect, the kind and volume of disperser and extraction solvents. In this research, the limit of detection (LOD) and quantification (LOQ) were 0.9 and 3 ng/mL for thiamine, 1.5 and 5 ng/mL for nicotinamide, 0.9 and 3 ng/mL for pyridoxine. The relative standard deviations (RSDs) were less than 2.87% (n=3). An appropriate linear behavior over the observed concentration range was obtained with the value of R²>0.996 for the target vitamins. This method was successfully applied to the sour cherry juice samples. Sour cherry var. Gise (Prunus cerasus var. Gise), which was used in this research, was a local variety of the sour cherry with large stone, double flowers, double fruits, dark red skin and dark red juice. This variety was identified in high altitude areas of Isfahan province after five years of study, since 2005, by Agricultural and Natural Resources Research Center of Isfahan.

No MeSH data available.


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The HPLC chromatograms of thiamine (B1), nicotinamide (B3) and pyridoxine (B6) for standard solutions. A: After extraction using DLLME; B: sour cherry juice without extraction; C: sour cherry juice after extraction using DLLME.
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Figure 1: The HPLC chromatograms of thiamine (B1), nicotinamide (B3) and pyridoxine (B6) for standard solutions. A: After extraction using DLLME; B: sour cherry juice without extraction; C: sour cherry juice after extraction using DLLME.

Mentions: The maximum UV spectra absorption of thiamine, nicotinamide and pyridoxine were 260 nm, therefore, the HPLC wavelength was adjusted at 260 nm. We used a C18 column and examined different concentrations of the aqueous phase (acetic acid water solution) and methanol. The optimum conditions were obtained to be: mobile phase consisted of 8% methanol and 92% aqueous phase (1 % (V/V) acetic acid water solution); pH 3.3. It had the sharpest peaks and the best resolution factors for thiamine, nicotinamide and pyridoxine. Figure 1A shows the HPLC chromatograms of thiamine, nicotinamide and pyridoxine for standard solutions after DLLME. The retention times of target vitamins were 4.6, 6.6 and 8.0 min for thiamine, nicotinamide and pyridoxine, respectively.


Determination of some B Vitamins in Sour Cherry Juice Using Dispersive Liquid-liquid Microextraction Followed by High-performance Liquid Chromatography.

Parsaei P, Bahmaei M, Ghannadi A - Iran J Pharm Res (2014)

The HPLC chromatograms of thiamine (B1), nicotinamide (B3) and pyridoxine (B6) for standard solutions. A: After extraction using DLLME; B: sour cherry juice without extraction; C: sour cherry juice after extraction using DLLME.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232812&req=5

Figure 1: The HPLC chromatograms of thiamine (B1), nicotinamide (B3) and pyridoxine (B6) for standard solutions. A: After extraction using DLLME; B: sour cherry juice without extraction; C: sour cherry juice after extraction using DLLME.
Mentions: The maximum UV spectra absorption of thiamine, nicotinamide and pyridoxine were 260 nm, therefore, the HPLC wavelength was adjusted at 260 nm. We used a C18 column and examined different concentrations of the aqueous phase (acetic acid water solution) and methanol. The optimum conditions were obtained to be: mobile phase consisted of 8% methanol and 92% aqueous phase (1 % (V/V) acetic acid water solution); pH 3.3. It had the sharpest peaks and the best resolution factors for thiamine, nicotinamide and pyridoxine. Figure 1A shows the HPLC chromatograms of thiamine, nicotinamide and pyridoxine for standard solutions after DLLME. The retention times of target vitamins were 4.6, 6.6 and 8.0 min for thiamine, nicotinamide and pyridoxine, respectively.

Bottom Line: The extraction efficiency of thiamine, nicotinamide and pyridoxine was influenced by factors such as: additional salt effect, the kind and volume of disperser and extraction solvents.Gise (Prunus cerasus var.This variety was identified in high altitude areas of Isfahan province after five years of study, since 2005, by Agricultural and Natural Resources Research Center of Isfahan.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Islamic Azad University, North Tehran Branch, Tehran, Iran.

ABSTRACT
Dispersive liquid-liquid microextraction method (DLLME) combined with high-performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to determine thiamine (B1), nicotinamide (B3) and pyridoxine (B6) in sour cherry juice. This method was rapid, simple and sensitive. Separation was accomplished using a C18 column. The optimum chromatographic conditions were found to be: mobile phase consisted of 8% methanol and 92% aqueous phase (1% (V/V) acetic acid water solution); flow rate, 0.7 mL/min; detection wavelength, 260 nm and pH, 3.3. The extraction efficiency of thiamine, nicotinamide and pyridoxine was influenced by factors such as: additional salt effect, the kind and volume of disperser and extraction solvents. In this research, the limit of detection (LOD) and quantification (LOQ) were 0.9 and 3 ng/mL for thiamine, 1.5 and 5 ng/mL for nicotinamide, 0.9 and 3 ng/mL for pyridoxine. The relative standard deviations (RSDs) were less than 2.87% (n=3). An appropriate linear behavior over the observed concentration range was obtained with the value of R²>0.996 for the target vitamins. This method was successfully applied to the sour cherry juice samples. Sour cherry var. Gise (Prunus cerasus var. Gise), which was used in this research, was a local variety of the sour cherry with large stone, double flowers, double fruits, dark red skin and dark red juice. This variety was identified in high altitude areas of Isfahan province after five years of study, since 2005, by Agricultural and Natural Resources Research Center of Isfahan.

No MeSH data available.


Related in: MedlinePlus