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Antioxidant, Metal Chelating, Anti-glucosidase Activities and Phytochemical Analysis of Selected Tropical Medicinal Plants.

Wong FC, Yong AL, Ting EP, Khoo SC, Ong HC, Chai TT - Iran J Pharm Res (2014)

Bottom Line: The levels of phenolic constituents in these medicinal plants were also quantified and compared.Our results suggested that C. nutans and C. formosana could potentially be used for the isolation of potent antioxidants and anti-diabetic compounds.To the best of our knowledge, this study represents the first time that C. nutans (Acanthaceae family) was reported in literature with glucosidase inhibition activity.

View Article: PubMed Central - PubMed

Affiliation: Centre for Biodiversity Research, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia. ; Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia.

ABSTRACT
The purpose of this investigation was to determine the antioxidant potentials and anti-glucosidase activities of six tropical medicinal plants. The levels of phenolic constituents in these medicinal plants were also quantified and compared. Antioxidation potentials were determined colorimetrically for scavenging activities against DPPH and NO radicals. Metal chelating assay was based on the measurement of iron-ferrozine absorbance at 562 nm. Anti-diabetic potentials were measured by using α-glucosidase as target enzyme. Medicinal plants' total phenolic, total flavonoid and hydroxycinnamic acid contents were determined using spectrophotometric methods, by comparison to standard plots prepared using gallic acid, quercetin and caffeic acid standards, respectively. Radical scavenging and metal chelating activities were detected in all medicinal plants, in concentration-dependent manners. Among the six plants tested, C. nutans, C. formosana and H. diffusa were found to possess α-glucosidase inhibitory activities. Spectrophotometric analysis indicated that the total phenolic, total flavonoid and hydroxycinnamic acid contents ranged from 12.13-21.39 mg GAE per g of dry sample, 1.83-9.86 mg QE per g of dry sample, and 0.91-2.74 mg CAE per g of dry sample, respectively. Our results suggested that C. nutans and C. formosana could potentially be used for the isolation of potent antioxidants and anti-diabetic compounds. To the best of our knowledge, this study represents the first time that C. nutans (Acanthaceae family) was reported in literature with glucosidase inhibition activity.

No MeSH data available.


Related in: MedlinePlus

NO radical scavenging activities of plant extracts at different concentrations. Data are reported as mean ± SE values (n=3).
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Figure 2: NO radical scavenging activities of plant extracts at different concentrations. Data are reported as mean ± SE values (n=3).

Mentions: In our DPPH radical scavenging assay, all plant extracts exhibited scavenging activities in a concentration-dependent manner, in the range of 1 to 16 mg/mL (Figure 1). Compared at the concentration of 16 mg/mL, C. formosana demonstrated the highest DPPH scavenging activity, while the lowest activity was detected in P. bleo. In addition, we tested our plant extracts in a NO radical scavenging assay. As NO radicals are found in biological systems, the results could be more biologically relevant. NO radical scavenging activities were detected in all plant extracts, in a concentration-dependent manner (Figure 2). Despite its low DPPH radical scavenging activity, P. bleo demonstrated strong NO radical scavenging activity, which was comparable to that of C. formosana and other plant extracts.


Antioxidant, Metal Chelating, Anti-glucosidase Activities and Phytochemical Analysis of Selected Tropical Medicinal Plants.

Wong FC, Yong AL, Ting EP, Khoo SC, Ong HC, Chai TT - Iran J Pharm Res (2014)

NO radical scavenging activities of plant extracts at different concentrations. Data are reported as mean ± SE values (n=3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232808&req=5

Figure 2: NO radical scavenging activities of plant extracts at different concentrations. Data are reported as mean ± SE values (n=3).
Mentions: In our DPPH radical scavenging assay, all plant extracts exhibited scavenging activities in a concentration-dependent manner, in the range of 1 to 16 mg/mL (Figure 1). Compared at the concentration of 16 mg/mL, C. formosana demonstrated the highest DPPH scavenging activity, while the lowest activity was detected in P. bleo. In addition, we tested our plant extracts in a NO radical scavenging assay. As NO radicals are found in biological systems, the results could be more biologically relevant. NO radical scavenging activities were detected in all plant extracts, in a concentration-dependent manner (Figure 2). Despite its low DPPH radical scavenging activity, P. bleo demonstrated strong NO radical scavenging activity, which was comparable to that of C. formosana and other plant extracts.

Bottom Line: The levels of phenolic constituents in these medicinal plants were also quantified and compared.Our results suggested that C. nutans and C. formosana could potentially be used for the isolation of potent antioxidants and anti-diabetic compounds.To the best of our knowledge, this study represents the first time that C. nutans (Acanthaceae family) was reported in literature with glucosidase inhibition activity.

View Article: PubMed Central - PubMed

Affiliation: Centre for Biodiversity Research, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia. ; Department of Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, 31900 Kampar, Malaysia.

ABSTRACT
The purpose of this investigation was to determine the antioxidant potentials and anti-glucosidase activities of six tropical medicinal plants. The levels of phenolic constituents in these medicinal plants were also quantified and compared. Antioxidation potentials were determined colorimetrically for scavenging activities against DPPH and NO radicals. Metal chelating assay was based on the measurement of iron-ferrozine absorbance at 562 nm. Anti-diabetic potentials were measured by using α-glucosidase as target enzyme. Medicinal plants' total phenolic, total flavonoid and hydroxycinnamic acid contents were determined using spectrophotometric methods, by comparison to standard plots prepared using gallic acid, quercetin and caffeic acid standards, respectively. Radical scavenging and metal chelating activities were detected in all medicinal plants, in concentration-dependent manners. Among the six plants tested, C. nutans, C. formosana and H. diffusa were found to possess α-glucosidase inhibitory activities. Spectrophotometric analysis indicated that the total phenolic, total flavonoid and hydroxycinnamic acid contents ranged from 12.13-21.39 mg GAE per g of dry sample, 1.83-9.86 mg QE per g of dry sample, and 0.91-2.74 mg CAE per g of dry sample, respectively. Our results suggested that C. nutans and C. formosana could potentially be used for the isolation of potent antioxidants and anti-diabetic compounds. To the best of our knowledge, this study represents the first time that C. nutans (Acanthaceae family) was reported in literature with glucosidase inhibition activity.

No MeSH data available.


Related in: MedlinePlus