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Structural characterisation of a polysaccharide from radix ranunculus ternati.

Huang X, Zhao Y, Jin X - Iran J Pharm Res (2014)

Bottom Line: And the absolute configuration of Glc was identified as D.The repetitive unit was likely composed of 3 As, 3 Cs, 13 Bs and 1 D.Together with the average molecular weight, it was predictable that HB-1 consisted of about seven of the repetitive unit.

View Article: PubMed Central - PubMed

Affiliation: Zhongshan Hospital, Xiamen University, Xiamen 361000, China .

ABSTRACT
A water soluble polysaccharide, HB-1, with a molecular weight of 23,930, was isolated from radix Ranunculi ternati. by hot water extraction, ethanol precipitation, deproteination,ultrafiltration and gel-filtration column chromatography. Its sugar composition was determined by GLC as Glc, Ara, and Gal in a molar ration of 16.071: 2.722: 1. And the absolute configuration of Glc was identified as D. Smith degradation and methylation reaction showed the proportion of -(1)Glc (A) was about 16%, -(1)Glc(4)- (B) about 62%, (C) about 14%, and -(1)Gal(6)- (D) about 8%. The repetitive unit was likely composed of 3 As, 3 Cs, 13 Bs and 1 D. Together with the average molecular weight, it was predictable that HB-1 consisted of about seven of the repetitive unit. The inhibition activity of HB-1 on human glioma cell line SF188 was also measured, only to find it inactive.

No MeSH data available.


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Repetitive unit of HB-1.
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Figure 3: Repetitive unit of HB-1.

Mentions: The lack of absorbance at 260 nm and 280 nm indicated that this polysaccharide contained no nucleic acid or protein. The sugar composition of HB-1 was determined by GC-MS as glucose (Glc), arabinose (Ara), and galactose (Gal) in a molar ratio of 16.071: 2.722: 1.000 and the absolute configuration of Glc were identified as D. The result of periodate oxidation and Smith degradation showed that the reaction products were mainly phycitol derived from (1→4) Glc and a few glycerine from (1→) Glc or Ara, (1→4) Ara, or (1→2) Ara. Methylation reaction showed that the proportion of —1Glc (A) was about 16%, —1Glc4— (B) about 62%, (C) about 14%, and —1Gal6— (D) about 8%. The proportion of A was almost the same as that of C, so came the presumption that there were 3 fragments of a attached to C, then C linked together with 13 Bs and 1 D, which was the unit that repeated to form the polysaccharide. The possible structure of the repetitive unit was shown in Figure 3. This unit had a molecular weight of 3350, according to the average molecular weight of the polysaccharide (23, 30), so it was predictable that HB-1 was composed of about seven of the repetitive unit.


Structural characterisation of a polysaccharide from radix ranunculus ternati.

Huang X, Zhao Y, Jin X - Iran J Pharm Res (2014)

Repetitive unit of HB-1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232807&req=5

Figure 3: Repetitive unit of HB-1.
Mentions: The lack of absorbance at 260 nm and 280 nm indicated that this polysaccharide contained no nucleic acid or protein. The sugar composition of HB-1 was determined by GC-MS as glucose (Glc), arabinose (Ara), and galactose (Gal) in a molar ratio of 16.071: 2.722: 1.000 and the absolute configuration of Glc were identified as D. The result of periodate oxidation and Smith degradation showed that the reaction products were mainly phycitol derived from (1→4) Glc and a few glycerine from (1→) Glc or Ara, (1→4) Ara, or (1→2) Ara. Methylation reaction showed that the proportion of —1Glc (A) was about 16%, —1Glc4— (B) about 62%, (C) about 14%, and —1Gal6— (D) about 8%. The proportion of A was almost the same as that of C, so came the presumption that there were 3 fragments of a attached to C, then C linked together with 13 Bs and 1 D, which was the unit that repeated to form the polysaccharide. The possible structure of the repetitive unit was shown in Figure 3. This unit had a molecular weight of 3350, according to the average molecular weight of the polysaccharide (23, 30), so it was predictable that HB-1 was composed of about seven of the repetitive unit.

Bottom Line: And the absolute configuration of Glc was identified as D.The repetitive unit was likely composed of 3 As, 3 Cs, 13 Bs and 1 D.Together with the average molecular weight, it was predictable that HB-1 consisted of about seven of the repetitive unit.

View Article: PubMed Central - PubMed

Affiliation: Zhongshan Hospital, Xiamen University, Xiamen 361000, China .

ABSTRACT
A water soluble polysaccharide, HB-1, with a molecular weight of 23,930, was isolated from radix Ranunculi ternati. by hot water extraction, ethanol precipitation, deproteination,ultrafiltration and gel-filtration column chromatography. Its sugar composition was determined by GLC as Glc, Ara, and Gal in a molar ration of 16.071: 2.722: 1. And the absolute configuration of Glc was identified as D. Smith degradation and methylation reaction showed the proportion of -(1)Glc (A) was about 16%, -(1)Glc(4)- (B) about 62%, (C) about 14%, and -(1)Gal(6)- (D) about 8%. The repetitive unit was likely composed of 3 As, 3 Cs, 13 Bs and 1 D. Together with the average molecular weight, it was predictable that HB-1 consisted of about seven of the repetitive unit. The inhibition activity of HB-1 on human glioma cell line SF188 was also measured, only to find it inactive.

No MeSH data available.


Related in: MedlinePlus