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Structural characterisation of a polysaccharide from radix ranunculus ternati.

Huang X, Zhao Y, Jin X - Iran J Pharm Res (2014)

Bottom Line: And the absolute configuration of Glc was identified as D.The repetitive unit was likely composed of 3 As, 3 Cs, 13 Bs and 1 D.Together with the average molecular weight, it was predictable that HB-1 consisted of about seven of the repetitive unit.

View Article: PubMed Central - PubMed

Affiliation: Zhongshan Hospital, Xiamen University, Xiamen 361000, China .

ABSTRACT
A water soluble polysaccharide, HB-1, with a molecular weight of 23,930, was isolated from radix Ranunculi ternati. by hot water extraction, ethanol precipitation, deproteination´╝îultrafiltration and gel-filtration column chromatography. Its sugar composition was determined by GLC as Glc, Ara, and Gal in a molar ration of 16.071: 2.722: 1. And the absolute configuration of Glc was identified as D. Smith degradation and methylation reaction showed the proportion of -(1)Glc (A) was about 16%, -(1)Glc(4)- (B) about 62%, (C) about 14%, and -(1)Gal(6)- (D) about 8%. The repetitive unit was likely composed of 3 As, 3 Cs, 13 Bs and 1 D. Together with the average molecular weight, it was predictable that HB-1 consisted of about seven of the repetitive unit. The inhibition activity of HB-1 on human glioma cell line SF188 was also measured, only to find it inactive.

No MeSH data available.


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Elution profile of HB-1.
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Figure 2: Elution profile of HB-1.

Mentions: We now report the isolation and structural elucidation of a neutral polysaccharide from radix Ranunculi ternate. The crude polysaccharides HA and HB were prepared from this herb by hot water extraction, EtOH precipitation, deproteinization, and ultrafiltration. HB-1 was obtained from HB by DEAE Sephadex A-50 (Figure 1) and Sephadex G100 gel-filtration. Its purity was examined on TSKgel G4000PWXL column, see Figure 2 for the elution profile. The average molecular weight examined of HB-1 is 23,930, which is consistent with the result of ultrafiltration, so our job is a successful example of the use of ultrafiltration in the isolation of polysaccharides.


Structural characterisation of a polysaccharide from radix ranunculus ternati.

Huang X, Zhao Y, Jin X - Iran J Pharm Res (2014)

Elution profile of HB-1.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232807&req=5

Figure 2: Elution profile of HB-1.
Mentions: We now report the isolation and structural elucidation of a neutral polysaccharide from radix Ranunculi ternate. The crude polysaccharides HA and HB were prepared from this herb by hot water extraction, EtOH precipitation, deproteinization, and ultrafiltration. HB-1 was obtained from HB by DEAE Sephadex A-50 (Figure 1) and Sephadex G100 gel-filtration. Its purity was examined on TSKgel G4000PWXL column, see Figure 2 for the elution profile. The average molecular weight examined of HB-1 is 23,930, which is consistent with the result of ultrafiltration, so our job is a successful example of the use of ultrafiltration in the isolation of polysaccharides.

Bottom Line: And the absolute configuration of Glc was identified as D.The repetitive unit was likely composed of 3 As, 3 Cs, 13 Bs and 1 D.Together with the average molecular weight, it was predictable that HB-1 consisted of about seven of the repetitive unit.

View Article: PubMed Central - PubMed

Affiliation: Zhongshan Hospital, Xiamen University, Xiamen 361000, China .

ABSTRACT
A water soluble polysaccharide, HB-1, with a molecular weight of 23,930, was isolated from radix Ranunculi ternati. by hot water extraction, ethanol precipitation, deproteination´╝îultrafiltration and gel-filtration column chromatography. Its sugar composition was determined by GLC as Glc, Ara, and Gal in a molar ration of 16.071: 2.722: 1. And the absolute configuration of Glc was identified as D. Smith degradation and methylation reaction showed the proportion of -(1)Glc (A) was about 16%, -(1)Glc(4)- (B) about 62%, (C) about 14%, and -(1)Gal(6)- (D) about 8%. The repetitive unit was likely composed of 3 As, 3 Cs, 13 Bs and 1 D. Together with the average molecular weight, it was predictable that HB-1 consisted of about seven of the repetitive unit. The inhibition activity of HB-1 on human glioma cell line SF188 was also measured, only to find it inactive.

No MeSH data available.


Related in: MedlinePlus