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Mcl-1 is up regulated by prenylated coumarin, umbelliprenin in jurkat cells.

Gholami O, Jeddi-Tehrani M, Iranshahi M, Zarnani AH, Ziai SA - Iran J Pharm Res (2014)

Bottom Line: We showed that, expression of Mcl-1 mRNA was increased from 1 hour to 3 hours incubation, but this increase has a scale down pattern.Moreover umbelliprenin could inhibit Mcl-1 protein.In conclusion umbelliprenin treatment modulates Mcl-1 expression at both the transcriptional and posttranslational levels.

View Article: PubMed Central - PubMed

Affiliation: Physiology and Pharmacology Department, Faculty of Medicine, Sabzevar University of Medical Sciences.

ABSTRACT
Chronic lymphocytic leukaemia (CLL) is the most common B-cell malignancy in the western world and exists as subtypes with very different clinical courses. Myeloid cell leukemia 1 (Mcl-1) is one member of Bcl-2 family proteins that has been shown to be expressed in various tissues and malignant cells, including CLL, where its expression is significantly associated with a failure to achieve complete remission following cytotoxic therapy. Induction of apoptosis by prenylated coumarin, umbelliprenin, in Jurkat cells was previously shown. We examined whether umbelliprenin can down-regulate Mcl-1 gene and protein in Jurkat cells. In this regard cells were incubated by umbelliprenin, and then down- regulation of Mcl-1 gene was studied by Real Time PCR method. Moreover, down-regulation of Mcl-1 protein was studied by western blot analysis. We showed that, expression of Mcl-1 mRNA was increased from 1 hour to 3 hours incubation, but this increase has a scale down pattern. Moreover umbelliprenin could inhibit Mcl-1 protein. In conclusion umbelliprenin treatment modulates Mcl-1 expression at both the transcriptional and posttranslational levels.

No MeSH data available.


Related in: MedlinePlus

Increase in relative Mcl-1 mRNA expression (Mcl-1 expression Treat/ Mcl-1 expression Control), by umbelliprenin (50 µM) on jurkat cells after 1, 2, 3 hours incubation. Mcl-1 levels were normalized to β actin. Only after 1 hour incubation this increase was significant (*P= 0.026).
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Figure 3: Increase in relative Mcl-1 mRNA expression (Mcl-1 expression Treat/ Mcl-1 expression Control), by umbelliprenin (50 µM) on jurkat cells after 1, 2, 3 hours incubation. Mcl-1 levels were normalized to β actin. Only after 1 hour incubation this increase was significant (*P= 0.026).

Mentions: Some studies have demonstrated that Mcl-1 mRNA is up-regulated as part of an initial rapid cellular response to cytotoxic stimuli such as chemotherapeutic agents, calcium ionophores, pneumococcal infection and UV irradiation. To determine whether umbelliprenin could similarly affect Mcl-1 gene expression in jurkat cells, we examined Mcl-1 mRNA levels. Real-time PCR was performed on treated jurkat cells for 1, 2 and 3 hours, and then data were analyzed by Rest-rg software. This software calculates relative Mcl-1 expression (Mcl-1 expression Treat/ Mcl-1 expression Control), and shows this relative expression as a unique column (Figure 3). In each case, Mcl-1 mRNA increased in response to umbelliprenin relative to control specimens (Figure 3). This increase is significant for 1 hour treatment. For 2 and 3 hours treatment, Mcl-1 mRNA increased but this increase is not significant. Thus, umbelliprenin falls into the category of apoptosis-inducing agents that initially up-regulates Mcl-1 mRNA.


Mcl-1 is up regulated by prenylated coumarin, umbelliprenin in jurkat cells.

Gholami O, Jeddi-Tehrani M, Iranshahi M, Zarnani AH, Ziai SA - Iran J Pharm Res (2014)

Increase in relative Mcl-1 mRNA expression (Mcl-1 expression Treat/ Mcl-1 expression Control), by umbelliprenin (50 µM) on jurkat cells after 1, 2, 3 hours incubation. Mcl-1 levels were normalized to β actin. Only after 1 hour incubation this increase was significant (*P= 0.026).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232805&req=5

Figure 3: Increase in relative Mcl-1 mRNA expression (Mcl-1 expression Treat/ Mcl-1 expression Control), by umbelliprenin (50 µM) on jurkat cells after 1, 2, 3 hours incubation. Mcl-1 levels were normalized to β actin. Only after 1 hour incubation this increase was significant (*P= 0.026).
Mentions: Some studies have demonstrated that Mcl-1 mRNA is up-regulated as part of an initial rapid cellular response to cytotoxic stimuli such as chemotherapeutic agents, calcium ionophores, pneumococcal infection and UV irradiation. To determine whether umbelliprenin could similarly affect Mcl-1 gene expression in jurkat cells, we examined Mcl-1 mRNA levels. Real-time PCR was performed on treated jurkat cells for 1, 2 and 3 hours, and then data were analyzed by Rest-rg software. This software calculates relative Mcl-1 expression (Mcl-1 expression Treat/ Mcl-1 expression Control), and shows this relative expression as a unique column (Figure 3). In each case, Mcl-1 mRNA increased in response to umbelliprenin relative to control specimens (Figure 3). This increase is significant for 1 hour treatment. For 2 and 3 hours treatment, Mcl-1 mRNA increased but this increase is not significant. Thus, umbelliprenin falls into the category of apoptosis-inducing agents that initially up-regulates Mcl-1 mRNA.

Bottom Line: We showed that, expression of Mcl-1 mRNA was increased from 1 hour to 3 hours incubation, but this increase has a scale down pattern.Moreover umbelliprenin could inhibit Mcl-1 protein.In conclusion umbelliprenin treatment modulates Mcl-1 expression at both the transcriptional and posttranslational levels.

View Article: PubMed Central - PubMed

Affiliation: Physiology and Pharmacology Department, Faculty of Medicine, Sabzevar University of Medical Sciences.

ABSTRACT
Chronic lymphocytic leukaemia (CLL) is the most common B-cell malignancy in the western world and exists as subtypes with very different clinical courses. Myeloid cell leukemia 1 (Mcl-1) is one member of Bcl-2 family proteins that has been shown to be expressed in various tissues and malignant cells, including CLL, where its expression is significantly associated with a failure to achieve complete remission following cytotoxic therapy. Induction of apoptosis by prenylated coumarin, umbelliprenin, in Jurkat cells was previously shown. We examined whether umbelliprenin can down-regulate Mcl-1 gene and protein in Jurkat cells. In this regard cells were incubated by umbelliprenin, and then down- regulation of Mcl-1 gene was studied by Real Time PCR method. Moreover, down-regulation of Mcl-1 protein was studied by western blot analysis. We showed that, expression of Mcl-1 mRNA was increased from 1 hour to 3 hours incubation, but this increase has a scale down pattern. Moreover umbelliprenin could inhibit Mcl-1 protein. In conclusion umbelliprenin treatment modulates Mcl-1 expression at both the transcriptional and posttranslational levels.

No MeSH data available.


Related in: MedlinePlus