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Mcl-1 is up regulated by prenylated coumarin, umbelliprenin in jurkat cells.

Gholami O, Jeddi-Tehrani M, Iranshahi M, Zarnani AH, Ziai SA - Iran J Pharm Res (2014)

Bottom Line: We showed that, expression of Mcl-1 mRNA was increased from 1 hour to 3 hours incubation, but this increase has a scale down pattern.Moreover umbelliprenin could inhibit Mcl-1 protein.In conclusion umbelliprenin treatment modulates Mcl-1 expression at both the transcriptional and posttranslational levels.

View Article: PubMed Central - PubMed

Affiliation: Physiology and Pharmacology Department, Faculty of Medicine, Sabzevar University of Medical Sciences.

ABSTRACT
Chronic lymphocytic leukaemia (CLL) is the most common B-cell malignancy in the western world and exists as subtypes with very different clinical courses. Myeloid cell leukemia 1 (Mcl-1) is one member of Bcl-2 family proteins that has been shown to be expressed in various tissues and malignant cells, including CLL, where its expression is significantly associated with a failure to achieve complete remission following cytotoxic therapy. Induction of apoptosis by prenylated coumarin, umbelliprenin, in Jurkat cells was previously shown. We examined whether umbelliprenin can down-regulate Mcl-1 gene and protein in Jurkat cells. In this regard cells were incubated by umbelliprenin, and then down- regulation of Mcl-1 gene was studied by Real Time PCR method. Moreover, down-regulation of Mcl-1 protein was studied by western blot analysis. We showed that, expression of Mcl-1 mRNA was increased from 1 hour to 3 hours incubation, but this increase has a scale down pattern. Moreover umbelliprenin could inhibit Mcl-1 protein. In conclusion umbelliprenin treatment modulates Mcl-1 expression at both the transcriptional and posttranslational levels.

No MeSH data available.


Related in: MedlinePlus

Changing in Mcl-1 protein expression by umbelliprenin (50 µM) on jurkat cells after 3, 6, 16 hours incubation. Umbelliprenin decreases Mcl-1 contents. β actin was used as a loading control. The ratio of each protein to β actin was calculated each time and showed as a column chart. Data are shown as mean ± standard deviation. *P<0.05.
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Figure 2: Changing in Mcl-1 protein expression by umbelliprenin (50 µM) on jurkat cells after 3, 6, 16 hours incubation. Umbelliprenin decreases Mcl-1 contents. β actin was used as a loading control. The ratio of each protein to β actin was calculated each time and showed as a column chart. Data are shown as mean ± standard deviation. *P<0.05.

Mentions: A modest increase in Mcl-1 protein was observed after 3 hours following umbelliprenin exposure, followed by a decrease in Mcl-1 protein within 6 hours that continued through 16 hours. The expression of Mcl-1 protein following umbelliprenin exposure appeared to be biphasic demonstrating a transient increase in Mcl-1 protein during the initial stages of an apoptotic response. Down-regulation of full length Mcl-1 protein was likely due to posttranslational regulation by caspases (Figure 2).


Mcl-1 is up regulated by prenylated coumarin, umbelliprenin in jurkat cells.

Gholami O, Jeddi-Tehrani M, Iranshahi M, Zarnani AH, Ziai SA - Iran J Pharm Res (2014)

Changing in Mcl-1 protein expression by umbelliprenin (50 µM) on jurkat cells after 3, 6, 16 hours incubation. Umbelliprenin decreases Mcl-1 contents. β actin was used as a loading control. The ratio of each protein to β actin was calculated each time and showed as a column chart. Data are shown as mean ± standard deviation. *P<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232805&req=5

Figure 2: Changing in Mcl-1 protein expression by umbelliprenin (50 µM) on jurkat cells after 3, 6, 16 hours incubation. Umbelliprenin decreases Mcl-1 contents. β actin was used as a loading control. The ratio of each protein to β actin was calculated each time and showed as a column chart. Data are shown as mean ± standard deviation. *P<0.05.
Mentions: A modest increase in Mcl-1 protein was observed after 3 hours following umbelliprenin exposure, followed by a decrease in Mcl-1 protein within 6 hours that continued through 16 hours. The expression of Mcl-1 protein following umbelliprenin exposure appeared to be biphasic demonstrating a transient increase in Mcl-1 protein during the initial stages of an apoptotic response. Down-regulation of full length Mcl-1 protein was likely due to posttranslational regulation by caspases (Figure 2).

Bottom Line: We showed that, expression of Mcl-1 mRNA was increased from 1 hour to 3 hours incubation, but this increase has a scale down pattern.Moreover umbelliprenin could inhibit Mcl-1 protein.In conclusion umbelliprenin treatment modulates Mcl-1 expression at both the transcriptional and posttranslational levels.

View Article: PubMed Central - PubMed

Affiliation: Physiology and Pharmacology Department, Faculty of Medicine, Sabzevar University of Medical Sciences.

ABSTRACT
Chronic lymphocytic leukaemia (CLL) is the most common B-cell malignancy in the western world and exists as subtypes with very different clinical courses. Myeloid cell leukemia 1 (Mcl-1) is one member of Bcl-2 family proteins that has been shown to be expressed in various tissues and malignant cells, including CLL, where its expression is significantly associated with a failure to achieve complete remission following cytotoxic therapy. Induction of apoptosis by prenylated coumarin, umbelliprenin, in Jurkat cells was previously shown. We examined whether umbelliprenin can down-regulate Mcl-1 gene and protein in Jurkat cells. In this regard cells were incubated by umbelliprenin, and then down- regulation of Mcl-1 gene was studied by Real Time PCR method. Moreover, down-regulation of Mcl-1 protein was studied by western blot analysis. We showed that, expression of Mcl-1 mRNA was increased from 1 hour to 3 hours incubation, but this increase has a scale down pattern. Moreover umbelliprenin could inhibit Mcl-1 protein. In conclusion umbelliprenin treatment modulates Mcl-1 expression at both the transcriptional and posttranslational levels.

No MeSH data available.


Related in: MedlinePlus