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Protective Effects of Lindera coreana on UVB-induced Oxidative Stress in Human HaCaT Keratinocytes.

Song JL, Gao Y - Iran J Pharm Res (2014)

Bottom Line: UVB significantly decreased the cell viability (p<0.05).LCE did not exhibit significantly cytotoxic effects and increased the viability of the HaCaT cells in a concentration-dependent manner.These results suggest that LCE exerted cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzymes activities.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, People's Republic of China. ; Department of Food Science and Nutrition, Pusan National University, Busan 609-735, South Korea.

ABSTRACT
The present study was designed to investigate the protective effect of ethanol extracts from Lindera coreana leaves (LCE) on UVB-induced oxidative stress in HaCaT keratinocytes. The HaCaT cells were pretreated with LCE for 24 h and then exposed to UVB (20 mJ/cm(2)) for 2 h. UVB significantly decreased the cell viability (p<0.05). LCE did not exhibit significantly cytotoxic effects and increased the viability of the HaCaT cells in a concentration-dependent manner. To further investigate the protective effects of LCE on UVB-induced oxidative stress in HaCaT cells, the cellular levels of reactive oxygen species (ROS), lipid peroxidation and endogenous antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px), were analyzed. LCE decreased the intracellular levels of ROS and lipid peroxidation and increased the activity of antioxidant enzymes. These results suggest that LCE exerted cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzymes activities. In addition, LCE also decreased the TNF-α, IL-1β and IL-6 levels in UVB-irradiated HaCaT cells.

No MeSH data available.


Related in: MedlinePlus

Effects of Lindera coreana leaf ethanol extracts (LCE) on intracellular reactive oxygen species (ROS) levels in UVB (20 mJ/cm2) irradiated HaCaT keratinocytes. Data are representative of three independent experiments as mean ± SD. a~e Mean values with different letters on the bars are significantly different (p < 0.05) according to Duncan’s multiple range test
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Figure 3: Effects of Lindera coreana leaf ethanol extracts (LCE) on intracellular reactive oxygen species (ROS) levels in UVB (20 mJ/cm2) irradiated HaCaT keratinocytes. Data are representative of three independent experiments as mean ± SD. a~e Mean values with different letters on the bars are significantly different (p < 0.05) according to Duncan’s multiple range test

Mentions: UV-irradiation induced ROS generation plays a very important role in the skin aging, inflammation and photocarcinogengesis (24). Increased ROS can break the intracellular antioxidant defense system, resulting in some biomacromolecules (such as DNA, lipid and protein) damage and cause cell death (25). Botanical antioxidants have been reported that to attenuate ROS generation and reduce UV-induced skin photoaging and photocarcinogenesis (26, 27). To investigate the protective effects of LCE in UVB-irradiated HaCaT cells, the intracellular ROS levels were evaluated using a fluorescent probe H2DCF-DA. As shown in Figure 3, UVB significantly increased the ROS generation compared with those in the normal cells. In the presence of UVB, LCE significantly reduced the ROS generations in a concentration-dependant manner between 10 and 200 ug/mL. Treatment with same concentrations of LCE alone did not significantly increase the intracellular ROS levels (data not shown). These results suggested that LCE is a free radical scavenger.


Protective Effects of Lindera coreana on UVB-induced Oxidative Stress in Human HaCaT Keratinocytes.

Song JL, Gao Y - Iran J Pharm Res (2014)

Effects of Lindera coreana leaf ethanol extracts (LCE) on intracellular reactive oxygen species (ROS) levels in UVB (20 mJ/cm2) irradiated HaCaT keratinocytes. Data are representative of three independent experiments as mean ± SD. a~e Mean values with different letters on the bars are significantly different (p < 0.05) according to Duncan’s multiple range test
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232803&req=5

Figure 3: Effects of Lindera coreana leaf ethanol extracts (LCE) on intracellular reactive oxygen species (ROS) levels in UVB (20 mJ/cm2) irradiated HaCaT keratinocytes. Data are representative of three independent experiments as mean ± SD. a~e Mean values with different letters on the bars are significantly different (p < 0.05) according to Duncan’s multiple range test
Mentions: UV-irradiation induced ROS generation plays a very important role in the skin aging, inflammation and photocarcinogengesis (24). Increased ROS can break the intracellular antioxidant defense system, resulting in some biomacromolecules (such as DNA, lipid and protein) damage and cause cell death (25). Botanical antioxidants have been reported that to attenuate ROS generation and reduce UV-induced skin photoaging and photocarcinogenesis (26, 27). To investigate the protective effects of LCE in UVB-irradiated HaCaT cells, the intracellular ROS levels were evaluated using a fluorescent probe H2DCF-DA. As shown in Figure 3, UVB significantly increased the ROS generation compared with those in the normal cells. In the presence of UVB, LCE significantly reduced the ROS generations in a concentration-dependant manner between 10 and 200 ug/mL. Treatment with same concentrations of LCE alone did not significantly increase the intracellular ROS levels (data not shown). These results suggested that LCE is a free radical scavenger.

Bottom Line: UVB significantly decreased the cell viability (p<0.05).LCE did not exhibit significantly cytotoxic effects and increased the viability of the HaCaT cells in a concentration-dependent manner.These results suggest that LCE exerted cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzymes activities.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, People's Republic of China. ; Department of Food Science and Nutrition, Pusan National University, Busan 609-735, South Korea.

ABSTRACT
The present study was designed to investigate the protective effect of ethanol extracts from Lindera coreana leaves (LCE) on UVB-induced oxidative stress in HaCaT keratinocytes. The HaCaT cells were pretreated with LCE for 24 h and then exposed to UVB (20 mJ/cm(2)) for 2 h. UVB significantly decreased the cell viability (p<0.05). LCE did not exhibit significantly cytotoxic effects and increased the viability of the HaCaT cells in a concentration-dependent manner. To further investigate the protective effects of LCE on UVB-induced oxidative stress in HaCaT cells, the cellular levels of reactive oxygen species (ROS), lipid peroxidation and endogenous antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px), were analyzed. LCE decreased the intracellular levels of ROS and lipid peroxidation and increased the activity of antioxidant enzymes. These results suggest that LCE exerted cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzymes activities. In addition, LCE also decreased the TNF-α, IL-1β and IL-6 levels in UVB-irradiated HaCaT cells.

No MeSH data available.


Related in: MedlinePlus