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Protective Effects of Lindera coreana on UVB-induced Oxidative Stress in Human HaCaT Keratinocytes.

Song JL, Gao Y - Iran J Pharm Res (2014)

Bottom Line: UVB significantly decreased the cell viability (p<0.05).LCE did not exhibit significantly cytotoxic effects and increased the viability of the HaCaT cells in a concentration-dependent manner.These results suggest that LCE exerted cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzymes activities.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, People's Republic of China. ; Department of Food Science and Nutrition, Pusan National University, Busan 609-735, South Korea.

ABSTRACT
The present study was designed to investigate the protective effect of ethanol extracts from Lindera coreana leaves (LCE) on UVB-induced oxidative stress in HaCaT keratinocytes. The HaCaT cells were pretreated with LCE for 24 h and then exposed to UVB (20 mJ/cm(2)) for 2 h. UVB significantly decreased the cell viability (p<0.05). LCE did not exhibit significantly cytotoxic effects and increased the viability of the HaCaT cells in a concentration-dependent manner. To further investigate the protective effects of LCE on UVB-induced oxidative stress in HaCaT cells, the cellular levels of reactive oxygen species (ROS), lipid peroxidation and endogenous antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px), were analyzed. LCE decreased the intracellular levels of ROS and lipid peroxidation and increased the activity of antioxidant enzymes. These results suggest that LCE exerted cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzymes activities. In addition, LCE also decreased the TNF-α, IL-1β and IL-6 levels in UVB-irradiated HaCaT cells.

No MeSH data available.


Related in: MedlinePlus

Effects of Lindera coreana leaf ethanol extracts (LCE) on cell viability in HaCaT keratinocytes. Data are representative of three independent experiments as mean ± SD. ns Means not significant
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Figure 1: Effects of Lindera coreana leaf ethanol extracts (LCE) on cell viability in HaCaT keratinocytes. Data are representative of three independent experiments as mean ± SD. ns Means not significant

Mentions: To investigate LCE-induced cytotoxicity, HaCaT cells were first treated with various concentrations of LCE (10-200 ug/mL) for 24 h and the cell viability was determined using MTT assay. LCE did not exhibited any significant cytotoxicity and the cell viability were both more than 90% (Figure 1). Based on these results, concentrations between 10 and 200 ug/mL were used to further studies. Human skin keratinocytes are essential cells in the skin and connective tissue, and are continuously exposed to UV irradiation (23). As shown in Figure 2, UVB (20 mJ/cm2) significantly induced the cell death in the HaCaT cells. However, following treatment with various concentrations of LCE, the cell viability was increased in a concentration-dependent manner. These results suggested that LCE exhibited a significantly protective effect which may be due to the free radical scavenging activity of LCE.


Protective Effects of Lindera coreana on UVB-induced Oxidative Stress in Human HaCaT Keratinocytes.

Song JL, Gao Y - Iran J Pharm Res (2014)

Effects of Lindera coreana leaf ethanol extracts (LCE) on cell viability in HaCaT keratinocytes. Data are representative of three independent experiments as mean ± SD. ns Means not significant
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232803&req=5

Figure 1: Effects of Lindera coreana leaf ethanol extracts (LCE) on cell viability in HaCaT keratinocytes. Data are representative of three independent experiments as mean ± SD. ns Means not significant
Mentions: To investigate LCE-induced cytotoxicity, HaCaT cells were first treated with various concentrations of LCE (10-200 ug/mL) for 24 h and the cell viability was determined using MTT assay. LCE did not exhibited any significant cytotoxicity and the cell viability were both more than 90% (Figure 1). Based on these results, concentrations between 10 and 200 ug/mL were used to further studies. Human skin keratinocytes are essential cells in the skin and connective tissue, and are continuously exposed to UV irradiation (23). As shown in Figure 2, UVB (20 mJ/cm2) significantly induced the cell death in the HaCaT cells. However, following treatment with various concentrations of LCE, the cell viability was increased in a concentration-dependent manner. These results suggested that LCE exhibited a significantly protective effect which may be due to the free radical scavenging activity of LCE.

Bottom Line: UVB significantly decreased the cell viability (p<0.05).LCE did not exhibit significantly cytotoxic effects and increased the viability of the HaCaT cells in a concentration-dependent manner.These results suggest that LCE exerted cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzymes activities.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, People's Republic of China. ; Department of Food Science and Nutrition, Pusan National University, Busan 609-735, South Korea.

ABSTRACT
The present study was designed to investigate the protective effect of ethanol extracts from Lindera coreana leaves (LCE) on UVB-induced oxidative stress in HaCaT keratinocytes. The HaCaT cells were pretreated with LCE for 24 h and then exposed to UVB (20 mJ/cm(2)) for 2 h. UVB significantly decreased the cell viability (p<0.05). LCE did not exhibit significantly cytotoxic effects and increased the viability of the HaCaT cells in a concentration-dependent manner. To further investigate the protective effects of LCE on UVB-induced oxidative stress in HaCaT cells, the cellular levels of reactive oxygen species (ROS), lipid peroxidation and endogenous antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-px), were analyzed. LCE decreased the intracellular levels of ROS and lipid peroxidation and increased the activity of antioxidant enzymes. These results suggest that LCE exerted cytoprotective activity against UVB-induced oxidative stress in HaCaT cells through the inhibition of lipid peroxidation, reduction of ROS levels and stimulation of antioxidant enzymes activities. In addition, LCE also decreased the TNF-α, IL-1β and IL-6 levels in UVB-irradiated HaCaT cells.

No MeSH data available.


Related in: MedlinePlus