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Econazole Nitrate Induces Apoptosis in MCF-7 Cells via Mitochondrial and Caspase Pathways.

Sun J, Yu CH, Zhao XL, Wang Y, Jiang SG, Gong XF - Iran J Pharm Res (2014)

Bottom Line: In addition, western blot analysis showed that EN resulted in the decrease expression of procaspase-3, procaspase-9 and bcl-2.In conclusion, these findings suggest that EN may be an effective way for treating human breast cancer.The anti-tumor mechanisms of EN might involve mitochondrial and caspase pathways.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080 , PR China.

ABSTRACT
Econazole nitrate (EN), a synthetic compound, is now in use as a routine antifungal drug. EN was shown to have antitumor effect, the tumor cell killing mechanisms, however, remain unclear. In this research, the apoptosis-inducing effect of EN on MCF-7 cells was investigated. The results showed that EN inhibited the proliferation of MCF-7 cells in a time- and dose-dependent manner by MTT method and colony forming assay. MCF-7 cells treated with EN showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Meanwhile, the loss of mitochondrial membrane potential was showed by flow cytometry. In addition, western blot analysis showed that EN resulted in the decrease expression of procaspase-3, procaspase-9 and bcl-2. In conclusion, these findings suggest that EN may be an effective way for treating human breast cancer. The anti-tumor mechanisms of EN might involve mitochondrial and caspase pathways.

No MeSH data available.


Related in: MedlinePlus

The expression of Bcl-2 in 0, 20, 40, 60 μM EN-treated MCF-7 cells. The cells were treated with EN for 48 h. Bcl-2 were analysed by western blot. GAPDH was used as an equal loading control
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Figure 7: The expression of Bcl-2 in 0, 20, 40, 60 μM EN-treated MCF-7 cells. The cells were treated with EN for 48 h. Bcl-2 were analysed by western blot. GAPDH was used as an equal loading control

Mentions: Mitochondrial Bcl-2 family is a series of proteins that regulate apoptosis and the Bcl-2 protein is an important regulator of the apoptotic cascade and promotes cell survival. We measured the expressions of Bcl-2 by western blot analysis. After incubation with EN, expression of Bcl-2 protein was decreased (Figure 5B). Based on the data, we can extrapolate that the intrinsic mitochondrial pathway may be involved in apoptosis induced by EN.


Econazole Nitrate Induces Apoptosis in MCF-7 Cells via Mitochondrial and Caspase Pathways.

Sun J, Yu CH, Zhao XL, Wang Y, Jiang SG, Gong XF - Iran J Pharm Res (2014)

The expression of Bcl-2 in 0, 20, 40, 60 μM EN-treated MCF-7 cells. The cells were treated with EN for 48 h. Bcl-2 were analysed by western blot. GAPDH was used as an equal loading control
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232799&req=5

Figure 7: The expression of Bcl-2 in 0, 20, 40, 60 μM EN-treated MCF-7 cells. The cells were treated with EN for 48 h. Bcl-2 were analysed by western blot. GAPDH was used as an equal loading control
Mentions: Mitochondrial Bcl-2 family is a series of proteins that regulate apoptosis and the Bcl-2 protein is an important regulator of the apoptotic cascade and promotes cell survival. We measured the expressions of Bcl-2 by western blot analysis. After incubation with EN, expression of Bcl-2 protein was decreased (Figure 5B). Based on the data, we can extrapolate that the intrinsic mitochondrial pathway may be involved in apoptosis induced by EN.

Bottom Line: In addition, western blot analysis showed that EN resulted in the decrease expression of procaspase-3, procaspase-9 and bcl-2.In conclusion, these findings suggest that EN may be an effective way for treating human breast cancer.The anti-tumor mechanisms of EN might involve mitochondrial and caspase pathways.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080 , PR China.

ABSTRACT
Econazole nitrate (EN), a synthetic compound, is now in use as a routine antifungal drug. EN was shown to have antitumor effect, the tumor cell killing mechanisms, however, remain unclear. In this research, the apoptosis-inducing effect of EN on MCF-7 cells was investigated. The results showed that EN inhibited the proliferation of MCF-7 cells in a time- and dose-dependent manner by MTT method and colony forming assay. MCF-7 cells treated with EN showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Meanwhile, the loss of mitochondrial membrane potential was showed by flow cytometry. In addition, western blot analysis showed that EN resulted in the decrease expression of procaspase-3, procaspase-9 and bcl-2. In conclusion, these findings suggest that EN may be an effective way for treating human breast cancer. The anti-tumor mechanisms of EN might involve mitochondrial and caspase pathways.

No MeSH data available.


Related in: MedlinePlus