Limits...
Econazole Nitrate Induces Apoptosis in MCF-7 Cells via Mitochondrial and Caspase Pathways.

Sun J, Yu CH, Zhao XL, Wang Y, Jiang SG, Gong XF - Iran J Pharm Res (2014)

Bottom Line: In addition, western blot analysis showed that EN resulted in the decrease expression of procaspase-3, procaspase-9 and bcl-2.In conclusion, these findings suggest that EN may be an effective way for treating human breast cancer.The anti-tumor mechanisms of EN might involve mitochondrial and caspase pathways.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080 , PR China.

ABSTRACT
Econazole nitrate (EN), a synthetic compound, is now in use as a routine antifungal drug. EN was shown to have antitumor effect, the tumor cell killing mechanisms, however, remain unclear. In this research, the apoptosis-inducing effect of EN on MCF-7 cells was investigated. The results showed that EN inhibited the proliferation of MCF-7 cells in a time- and dose-dependent manner by MTT method and colony forming assay. MCF-7 cells treated with EN showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Meanwhile, the loss of mitochondrial membrane potential was showed by flow cytometry. In addition, western blot analysis showed that EN resulted in the decrease expression of procaspase-3, procaspase-9 and bcl-2. In conclusion, these findings suggest that EN may be an effective way for treating human breast cancer. The anti-tumor mechanisms of EN might involve mitochondrial and caspase pathways.

No MeSH data available.


Related in: MedlinePlus

DNA fragmentation induced by EN in MCF-7 cells. The cells were treated with varied concentrations of EN (0, 20, 40 and 60 μM) for 48 h (A)or treated with 40 μM for 24 h, 48 h and 72 h (B). DNA was isolated by agarose gel electrophoresis and analyzed by ethidium bromide staining.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4232799&req=5

Figure 5: DNA fragmentation induced by EN in MCF-7 cells. The cells were treated with varied concentrations of EN (0, 20, 40 and 60 μM) for 48 h (A)or treated with 40 μM for 24 h, 48 h and 72 h (B). DNA was isolated by agarose gel electrophoresis and analyzed by ethidium bromide staining.

Mentions: One of the specific characteristics of cell apoptosis is DNA fragmentation, which leads to the appearance of DNA ladder. The results showed that DNA ladder was obvious after 40 and 60 µM when treated with EN for 48 h (Figure 4B, left). Additionally, DNA ladder was obvious after 40 µM treatment for 48 and 72 h (Figure 4B, right), which further confirmed that EN induced cell apoptosis.


Econazole Nitrate Induces Apoptosis in MCF-7 Cells via Mitochondrial and Caspase Pathways.

Sun J, Yu CH, Zhao XL, Wang Y, Jiang SG, Gong XF - Iran J Pharm Res (2014)

DNA fragmentation induced by EN in MCF-7 cells. The cells were treated with varied concentrations of EN (0, 20, 40 and 60 μM) for 48 h (A)or treated with 40 μM for 24 h, 48 h and 72 h (B). DNA was isolated by agarose gel electrophoresis and analyzed by ethidium bromide staining.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232799&req=5

Figure 5: DNA fragmentation induced by EN in MCF-7 cells. The cells were treated with varied concentrations of EN (0, 20, 40 and 60 μM) for 48 h (A)or treated with 40 μM for 24 h, 48 h and 72 h (B). DNA was isolated by agarose gel electrophoresis and analyzed by ethidium bromide staining.
Mentions: One of the specific characteristics of cell apoptosis is DNA fragmentation, which leads to the appearance of DNA ladder. The results showed that DNA ladder was obvious after 40 and 60 µM when treated with EN for 48 h (Figure 4B, left). Additionally, DNA ladder was obvious after 40 µM treatment for 48 and 72 h (Figure 4B, right), which further confirmed that EN induced cell apoptosis.

Bottom Line: In addition, western blot analysis showed that EN resulted in the decrease expression of procaspase-3, procaspase-9 and bcl-2.In conclusion, these findings suggest that EN may be an effective way for treating human breast cancer.The anti-tumor mechanisms of EN might involve mitochondrial and caspase pathways.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080 , PR China.

ABSTRACT
Econazole nitrate (EN), a synthetic compound, is now in use as a routine antifungal drug. EN was shown to have antitumor effect, the tumor cell killing mechanisms, however, remain unclear. In this research, the apoptosis-inducing effect of EN on MCF-7 cells was investigated. The results showed that EN inhibited the proliferation of MCF-7 cells in a time- and dose-dependent manner by MTT method and colony forming assay. MCF-7 cells treated with EN showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Meanwhile, the loss of mitochondrial membrane potential was showed by flow cytometry. In addition, western blot analysis showed that EN resulted in the decrease expression of procaspase-3, procaspase-9 and bcl-2. In conclusion, these findings suggest that EN may be an effective way for treating human breast cancer. The anti-tumor mechanisms of EN might involve mitochondrial and caspase pathways.

No MeSH data available.


Related in: MedlinePlus