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Econazole Nitrate Induces Apoptosis in MCF-7 Cells via Mitochondrial and Caspase Pathways.

Sun J, Yu CH, Zhao XL, Wang Y, Jiang SG, Gong XF - Iran J Pharm Res (2014)

Bottom Line: In addition, western blot analysis showed that EN resulted in the decrease expression of procaspase-3, procaspase-9 and bcl-2.In conclusion, these findings suggest that EN may be an effective way for treating human breast cancer.The anti-tumor mechanisms of EN might involve mitochondrial and caspase pathways.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080 , PR China.

ABSTRACT
Econazole nitrate (EN), a synthetic compound, is now in use as a routine antifungal drug. EN was shown to have antitumor effect, the tumor cell killing mechanisms, however, remain unclear. In this research, the apoptosis-inducing effect of EN on MCF-7 cells was investigated. The results showed that EN inhibited the proliferation of MCF-7 cells in a time- and dose-dependent manner by MTT method and colony forming assay. MCF-7 cells treated with EN showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Meanwhile, the loss of mitochondrial membrane potential was showed by flow cytometry. In addition, western blot analysis showed that EN resulted in the decrease expression of procaspase-3, procaspase-9 and bcl-2. In conclusion, these findings suggest that EN may be an effective way for treating human breast cancer. The anti-tumor mechanisms of EN might involve mitochondrial and caspase pathways.

No MeSH data available.


Related in: MedlinePlus

EN induces cell growth inhibition. The MCF-7 cells were exposed to EN at concentration ranging from 0-120 μM and incubated for 24 h (◆), 48 h (■) and 72 h (▲). The inhibitiory ratio was measured by MTT assay. Data represent the mean ±SD of three independent experiments
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Figure 2: EN induces cell growth inhibition. The MCF-7 cells were exposed to EN at concentration ranging from 0-120 μM and incubated for 24 h (◆), 48 h (■) and 72 h (▲). The inhibitiory ratio was measured by MTT assay. Data represent the mean ±SD of three independent experiments

Mentions: To investigate the potential effects of EN on the growth and viability of human breast carcinoma MCF-7 cells, the cells were treated with EN in varying concentrations(0-120 μM), and over varying lengths of time (24-72 h), then assessed by MTT assay. EN significantly reduced cell viability in a time- and dose-dependent manner in MCF-7 cells (Figure 2), and the IC50 value for MCF-7 cells is 45 μΜ for 48 h.


Econazole Nitrate Induces Apoptosis in MCF-7 Cells via Mitochondrial and Caspase Pathways.

Sun J, Yu CH, Zhao XL, Wang Y, Jiang SG, Gong XF - Iran J Pharm Res (2014)

EN induces cell growth inhibition. The MCF-7 cells were exposed to EN at concentration ranging from 0-120 μM and incubated for 24 h (◆), 48 h (■) and 72 h (▲). The inhibitiory ratio was measured by MTT assay. Data represent the mean ±SD of three independent experiments
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232799&req=5

Figure 2: EN induces cell growth inhibition. The MCF-7 cells were exposed to EN at concentration ranging from 0-120 μM and incubated for 24 h (◆), 48 h (■) and 72 h (▲). The inhibitiory ratio was measured by MTT assay. Data represent the mean ±SD of three independent experiments
Mentions: To investigate the potential effects of EN on the growth and viability of human breast carcinoma MCF-7 cells, the cells were treated with EN in varying concentrations(0-120 μM), and over varying lengths of time (24-72 h), then assessed by MTT assay. EN significantly reduced cell viability in a time- and dose-dependent manner in MCF-7 cells (Figure 2), and the IC50 value for MCF-7 cells is 45 μΜ for 48 h.

Bottom Line: In addition, western blot analysis showed that EN resulted in the decrease expression of procaspase-3, procaspase-9 and bcl-2.In conclusion, these findings suggest that EN may be an effective way for treating human breast cancer.The anti-tumor mechanisms of EN might involve mitochondrial and caspase pathways.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080 , PR China.

ABSTRACT
Econazole nitrate (EN), a synthetic compound, is now in use as a routine antifungal drug. EN was shown to have antitumor effect, the tumor cell killing mechanisms, however, remain unclear. In this research, the apoptosis-inducing effect of EN on MCF-7 cells was investigated. The results showed that EN inhibited the proliferation of MCF-7 cells in a time- and dose-dependent manner by MTT method and colony forming assay. MCF-7 cells treated with EN showed typical characteristics of apoptosis including the morphological changes and DNA fragmentation. Meanwhile, the loss of mitochondrial membrane potential was showed by flow cytometry. In addition, western blot analysis showed that EN resulted in the decrease expression of procaspase-3, procaspase-9 and bcl-2. In conclusion, these findings suggest that EN may be an effective way for treating human breast cancer. The anti-tumor mechanisms of EN might involve mitochondrial and caspase pathways.

No MeSH data available.


Related in: MedlinePlus