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Role of Fructose as a Potent Antiarrhythmic and Anti-infarct agent in Isolated Rat Heart.

Haghighat Azari M, Najafi M - Iran J Pharm Res (2014)

Bottom Line: In addition, total number of ventricular ectopic beats were significantly decreased by all used concentrations of fructose (P<0.01 for group 2, P<0.001 for groups 3 and 4, respectively).Fructose also produced significant decrease in the number, incidence and duration of ventricular tachycardia compared to the control (P<0.05).Alterations in glycogen storage and/or glycolytic efficiency may probably involve in these cardioprotective effects.

View Article: PubMed Central - PubMed

Affiliation: Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

ABSTRACT
In the current study, effects of acute short term administration of fructose on cardiac arrhythmias and myocardial infarction size following ischemia/reperfusion were investigated in isolated rat heart. The hearts were subjected to 30 min zero flow global ischemia followed by 120 min reperfusion. In the control group, the hearts were perfused by normal drug free Krebs-Henseleit (K/H) solution throughout the experiments, while in the treated groups (2-4), they were perfused with fructose containing K/H solution at 12, 24 and 48 mM concentrations during stabilization and reperfusion time, respectively. Cardiac arrhythmias were determined based on the Lambeth conventions and the infarct size was measured by computerized planimetry. Myocardial infarction size was 22 ± 3% in the control group, however administration of fructose (12, 24 and 48 mM) reduced it to 15 ± 3 (P<0.05), 7±2 (P<0.001) and 4 ± 2% (P<0.001), respectively. A direct linear correlation between fructose concentrations and infarction size reduction was observed (R(2)=0.970). In addition, total number of ventricular ectopic beats were significantly decreased by all used concentrations of fructose (P<0.01 for group 2, P<0.001 for groups 3 and 4, respectively). Fructose also produced significant decrease in the number, incidence and duration of ventricular tachycardia compared to the control (P<0.05). The data showed that acute short term administration of fructose can protect isolated rat heart against ischemia/reperfusion injuries as reduction of infarct size and cardiac arrhythmias. Alterations in glycogen storage and/or glycolytic efficiency may probably involve in these cardioprotective effects. Also it is possible that fructose can act as a pharmacological preconditioning agent.

No MeSH data available.


Related in: MedlinePlus

Relationship between myocardial infarct size reduction and fructose concentrations (0-48 mM) in isolated rat hearts. Data are represented as Mean±SEM
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Figure 4: Relationship between myocardial infarct size reduction and fructose concentrations (0-48 mM) in isolated rat hearts. Data are represented as Mean±SEM

Mentions: Effects of fructose on the myocardial infarction size are summarized in Table 2. Perfusion of the isolated rat hearts by K/H solution containing fructose markedly reduced infarct size by the all used concentrations. As shown in Figure 3, the infarct size was 22 ± 3% in the control group, while fructose (12, 24 and 48 mM) produced significant reduction in the size of myocardial infarction from the control value to 15 ± 3% (P<0.05), 7 ± 2% (P<0.001) and 4 ± 2% (P<0.001), respectively. In addition, administration of fructose significantly decreased the infarcted volume of ischemic hearts in comparison with the control group (Table 2). Furthermore, a direct linear correlation (R2=0.970) between fructose concentrations and reduction of infarct size was observed (Figure 4). Sample staining images to determine infarction size by TTC method in isolated rat heart have been illustrated in Figure 5.


Role of Fructose as a Potent Antiarrhythmic and Anti-infarct agent in Isolated Rat Heart.

Haghighat Azari M, Najafi M - Iran J Pharm Res (2014)

Relationship between myocardial infarct size reduction and fructose concentrations (0-48 mM) in isolated rat hearts. Data are represented as Mean±SEM
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232796&req=5

Figure 4: Relationship between myocardial infarct size reduction and fructose concentrations (0-48 mM) in isolated rat hearts. Data are represented as Mean±SEM
Mentions: Effects of fructose on the myocardial infarction size are summarized in Table 2. Perfusion of the isolated rat hearts by K/H solution containing fructose markedly reduced infarct size by the all used concentrations. As shown in Figure 3, the infarct size was 22 ± 3% in the control group, while fructose (12, 24 and 48 mM) produced significant reduction in the size of myocardial infarction from the control value to 15 ± 3% (P<0.05), 7 ± 2% (P<0.001) and 4 ± 2% (P<0.001), respectively. In addition, administration of fructose significantly decreased the infarcted volume of ischemic hearts in comparison with the control group (Table 2). Furthermore, a direct linear correlation (R2=0.970) between fructose concentrations and reduction of infarct size was observed (Figure 4). Sample staining images to determine infarction size by TTC method in isolated rat heart have been illustrated in Figure 5.

Bottom Line: In addition, total number of ventricular ectopic beats were significantly decreased by all used concentrations of fructose (P<0.01 for group 2, P<0.001 for groups 3 and 4, respectively).Fructose also produced significant decrease in the number, incidence and duration of ventricular tachycardia compared to the control (P<0.05).Alterations in glycogen storage and/or glycolytic efficiency may probably involve in these cardioprotective effects.

View Article: PubMed Central - PubMed

Affiliation: Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

ABSTRACT
In the current study, effects of acute short term administration of fructose on cardiac arrhythmias and myocardial infarction size following ischemia/reperfusion were investigated in isolated rat heart. The hearts were subjected to 30 min zero flow global ischemia followed by 120 min reperfusion. In the control group, the hearts were perfused by normal drug free Krebs-Henseleit (K/H) solution throughout the experiments, while in the treated groups (2-4), they were perfused with fructose containing K/H solution at 12, 24 and 48 mM concentrations during stabilization and reperfusion time, respectively. Cardiac arrhythmias were determined based on the Lambeth conventions and the infarct size was measured by computerized planimetry. Myocardial infarction size was 22 ± 3% in the control group, however administration of fructose (12, 24 and 48 mM) reduced it to 15 ± 3 (P<0.05), 7±2 (P<0.001) and 4 ± 2% (P<0.001), respectively. A direct linear correlation between fructose concentrations and infarction size reduction was observed (R(2)=0.970). In addition, total number of ventricular ectopic beats were significantly decreased by all used concentrations of fructose (P<0.01 for group 2, P<0.001 for groups 3 and 4, respectively). Fructose also produced significant decrease in the number, incidence and duration of ventricular tachycardia compared to the control (P<0.05). The data showed that acute short term administration of fructose can protect isolated rat heart against ischemia/reperfusion injuries as reduction of infarct size and cardiac arrhythmias. Alterations in glycogen storage and/or glycolytic efficiency may probably involve in these cardioprotective effects. Also it is possible that fructose can act as a pharmacological preconditioning agent.

No MeSH data available.


Related in: MedlinePlus