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Role of Fructose as a Potent Antiarrhythmic and Anti-infarct agent in Isolated Rat Heart.

Haghighat Azari M, Najafi M - Iran J Pharm Res (2014)

Bottom Line: In addition, total number of ventricular ectopic beats were significantly decreased by all used concentrations of fructose (P<0.01 for group 2, P<0.001 for groups 3 and 4, respectively).Fructose also produced significant decrease in the number, incidence and duration of ventricular tachycardia compared to the control (P<0.05).Alterations in glycogen storage and/or glycolytic efficiency may probably involve in these cardioprotective effects.

View Article: PubMed Central - PubMed

Affiliation: Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

ABSTRACT
In the current study, effects of acute short term administration of fructose on cardiac arrhythmias and myocardial infarction size following ischemia/reperfusion were investigated in isolated rat heart. The hearts were subjected to 30 min zero flow global ischemia followed by 120 min reperfusion. In the control group, the hearts were perfused by normal drug free Krebs-Henseleit (K/H) solution throughout the experiments, while in the treated groups (2-4), they were perfused with fructose containing K/H solution at 12, 24 and 48 mM concentrations during stabilization and reperfusion time, respectively. Cardiac arrhythmias were determined based on the Lambeth conventions and the infarct size was measured by computerized planimetry. Myocardial infarction size was 22 ± 3% in the control group, however administration of fructose (12, 24 and 48 mM) reduced it to 15 ± 3 (P<0.05), 7±2 (P<0.001) and 4 ± 2% (P<0.001), respectively. A direct linear correlation between fructose concentrations and infarction size reduction was observed (R(2)=0.970). In addition, total number of ventricular ectopic beats were significantly decreased by all used concentrations of fructose (P<0.01 for group 2, P<0.001 for groups 3 and 4, respectively). Fructose also produced significant decrease in the number, incidence and duration of ventricular tachycardia compared to the control (P<0.05). The data showed that acute short term administration of fructose can protect isolated rat heart against ischemia/reperfusion injuries as reduction of infarct size and cardiac arrhythmias. Alterations in glycogen storage and/or glycolytic efficiency may probably involve in these cardioprotective effects. Also it is possible that fructose can act as a pharmacological preconditioning agent.

No MeSH data available.


Related in: MedlinePlus

The total number of ventricular ectopic beats (VEBs: Single+Salvos+VT) in the control and treated groups receiving 12, 24 and 48 mM of fructose in K/H solution (groups 2, 3 and 4). Data are represented as Mean ± SEM. **P<0.01, ***P<0.001 versus the control group. N=7-10 in each group
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Figure 1: The total number of ventricular ectopic beats (VEBs: Single+Salvos+VT) in the control and treated groups receiving 12, 24 and 48 mM of fructose in K/H solution (groups 2, 3 and 4). Data are represented as Mean ± SEM. **P<0.01, ***P<0.001 versus the control group. N=7-10 in each group

Mentions: The effects of acute high concentrations of fructose against reperfusion-induced cardiac arrhythmias after 30 min zero flow global ischemia are summarized in Table 1. As shown in the table and Figure 1, perfusion of K/H solution containing fructose in groups 2, 3 and 4 significantly decreased the total number of VEBs. In the control group, number of VEBs was 77 ± 4, while the value was decreased by administration of fructose (12, 24 and 48 mM) to 50 ± 7 (P<0.01), 46 ± 4 (P<0.001) and 29 ± 2 (P<0.001), respectively. As illustrated in Figure 2, using fructose also markedly lowered the number of single ectopic beats compared to the control group (P<0.01 for group 3, P<0.001 for groups 2 and 4, respectively). Likewise, fructose at 24 and 48 mM concentrations produced significant decrease (P<0.05) in the number, incidence and duration of VT compared to the control value (Table 1). At the same time, it had no significant reduction in the number of salvos arrhythmias. The time spent in reversible VF was 27 ± 14 sec in the control group, however administration of fructose reduced VF duration not statistically significant. In addition, fructose containing K/H solution lowered the incidence of total VF by 12, 24 and 48 mM, although the effect was not significant compared to the control group (Table 1).


Role of Fructose as a Potent Antiarrhythmic and Anti-infarct agent in Isolated Rat Heart.

Haghighat Azari M, Najafi M - Iran J Pharm Res (2014)

The total number of ventricular ectopic beats (VEBs: Single+Salvos+VT) in the control and treated groups receiving 12, 24 and 48 mM of fructose in K/H solution (groups 2, 3 and 4). Data are represented as Mean ± SEM. **P<0.01, ***P<0.001 versus the control group. N=7-10 in each group
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232796&req=5

Figure 1: The total number of ventricular ectopic beats (VEBs: Single+Salvos+VT) in the control and treated groups receiving 12, 24 and 48 mM of fructose in K/H solution (groups 2, 3 and 4). Data are represented as Mean ± SEM. **P<0.01, ***P<0.001 versus the control group. N=7-10 in each group
Mentions: The effects of acute high concentrations of fructose against reperfusion-induced cardiac arrhythmias after 30 min zero flow global ischemia are summarized in Table 1. As shown in the table and Figure 1, perfusion of K/H solution containing fructose in groups 2, 3 and 4 significantly decreased the total number of VEBs. In the control group, number of VEBs was 77 ± 4, while the value was decreased by administration of fructose (12, 24 and 48 mM) to 50 ± 7 (P<0.01), 46 ± 4 (P<0.001) and 29 ± 2 (P<0.001), respectively. As illustrated in Figure 2, using fructose also markedly lowered the number of single ectopic beats compared to the control group (P<0.01 for group 3, P<0.001 for groups 2 and 4, respectively). Likewise, fructose at 24 and 48 mM concentrations produced significant decrease (P<0.05) in the number, incidence and duration of VT compared to the control value (Table 1). At the same time, it had no significant reduction in the number of salvos arrhythmias. The time spent in reversible VF was 27 ± 14 sec in the control group, however administration of fructose reduced VF duration not statistically significant. In addition, fructose containing K/H solution lowered the incidence of total VF by 12, 24 and 48 mM, although the effect was not significant compared to the control group (Table 1).

Bottom Line: In addition, total number of ventricular ectopic beats were significantly decreased by all used concentrations of fructose (P<0.01 for group 2, P<0.001 for groups 3 and 4, respectively).Fructose also produced significant decrease in the number, incidence and duration of ventricular tachycardia compared to the control (P<0.05).Alterations in glycogen storage and/or glycolytic efficiency may probably involve in these cardioprotective effects.

View Article: PubMed Central - PubMed

Affiliation: Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.

ABSTRACT
In the current study, effects of acute short term administration of fructose on cardiac arrhythmias and myocardial infarction size following ischemia/reperfusion were investigated in isolated rat heart. The hearts were subjected to 30 min zero flow global ischemia followed by 120 min reperfusion. In the control group, the hearts were perfused by normal drug free Krebs-Henseleit (K/H) solution throughout the experiments, while in the treated groups (2-4), they were perfused with fructose containing K/H solution at 12, 24 and 48 mM concentrations during stabilization and reperfusion time, respectively. Cardiac arrhythmias were determined based on the Lambeth conventions and the infarct size was measured by computerized planimetry. Myocardial infarction size was 22 ± 3% in the control group, however administration of fructose (12, 24 and 48 mM) reduced it to 15 ± 3 (P<0.05), 7±2 (P<0.001) and 4 ± 2% (P<0.001), respectively. A direct linear correlation between fructose concentrations and infarction size reduction was observed (R(2)=0.970). In addition, total number of ventricular ectopic beats were significantly decreased by all used concentrations of fructose (P<0.01 for group 2, P<0.001 for groups 3 and 4, respectively). Fructose also produced significant decrease in the number, incidence and duration of ventricular tachycardia compared to the control (P<0.05). The data showed that acute short term administration of fructose can protect isolated rat heart against ischemia/reperfusion injuries as reduction of infarct size and cardiac arrhythmias. Alterations in glycogen storage and/or glycolytic efficiency may probably involve in these cardioprotective effects. Also it is possible that fructose can act as a pharmacological preconditioning agent.

No MeSH data available.


Related in: MedlinePlus