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The Effects of Extending of Co-planarity in a Series of Structurally Relative Polypyridyl Palladium(II) Complexes on DNA-binding and Cytotoxicity Properties.

Shahraki S, Mansouri-Torshizi H, Sori Nezami Z, Ghahghaei A, Yaghoubi F, Divsalar A, Saboury AA, H Shirazi F - Iran J Pharm Res (2014)

Bottom Line: EBr and Scatchard experiments for a-d complexes suggest efficient intercalative binding affinity to CT-DNA giving the order: d > c > b > a.Several binding and thermodynamic parameters are also described.The biological activity of these cationic and water soluble palladium complexes were tested against chronic myelogenous leukemia cell line, K562. b, c and d complexes show cytotoxic concentration (Cc50) values much lower than cisplatin.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Sistan & Baluchestan, Zahedan, Iran.

ABSTRACT
In depth interaction studies between calf thymus deoxyribonucleic acid (CT-DNA) and a series of four structurally relative palladium(II) complexes [Pd(en)(HB)](NO3)2 (a-d), where en is ethylenediamine and heterocyclic base (HB) is 2,2'-bipyridine (bpy, a); 1,10-phenanthroline (phen, b); dipyridoquinoxaline (dpq, c) and dipyridophenazine (dppz, d) (Figure 1), were performed. These studies have been investigated by utilizing the electronic absorption spectroscopy, fluorescence spectra and ethidium bromide (EBr) displacement and gel filtration techniques. a-d complexes cooperatively bind and denature the DNA at low concentrations. Their concentration at midpoint of transition, L1/2, follows the order a > b > c > d. Also the g, the number of binding sites per 1000 nucleotides, follows the order a > b ~ c > d. EBr and Scatchard experiments for a-d complexes suggest efficient intercalative binding affinity to CT-DNA giving the order: d > c > b > a. Several binding and thermodynamic parameters are also described. The biological activity of these cationic and water soluble palladium complexes were tested against chronic myelogenous leukemia cell line, K562. b, c and d complexes show cytotoxic concentration (Cc50) values much lower than cisplatin.

No MeSH data available.


Related in: MedlinePlus

Scatchard plots for binding of [Pd(en)(bpy)](NO3)2 a, [Pd(en)(phen)](NO3)2 b, [Pd(en)(dpq)](NO3)2 c and [Pd(en)(dppz)](NO3)2 d, with CT-DNA
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Figure 7: Scatchard plots for binding of [Pd(en)(bpy)](NO3)2 a, [Pd(en)(phen)](NO3)2 b, [Pd(en)(dpq)](NO3)2 c and [Pd(en)(dppz)](NO3)2 d, with CT-DNA

Mentions: In another experiment, a fixed amount of DNA was titrated with varying amount of each metal complex. The concentration of each metal complex bound to DNA, [L]b, and the concentration of each free metal complex, [L]f , are calculated by using the relationship [L]b=ΔA[L]f/ΔAmax. Here [L]f = [L]t –[L]b where [L]t is the maximum concentration of each metal complex added to saturate all the binding sites of DNA and ν is the ratio of the concentration of bound metal complex to total [DNA]. Using these data (ν, [L]f ), the Scatchard plots were constructed for the interaction of each metal complex at the two temperatures 300 K and 310 K. The Scatchard plots are shown in Figure 7 for a-d complexes. These plots are curvilinear concave downwards, suggesting cooperative binding (26).


The Effects of Extending of Co-planarity in a Series of Structurally Relative Polypyridyl Palladium(II) Complexes on DNA-binding and Cytotoxicity Properties.

Shahraki S, Mansouri-Torshizi H, Sori Nezami Z, Ghahghaei A, Yaghoubi F, Divsalar A, Saboury AA, H Shirazi F - Iran J Pharm Res (2014)

Scatchard plots for binding of [Pd(en)(bpy)](NO3)2 a, [Pd(en)(phen)](NO3)2 b, [Pd(en)(dpq)](NO3)2 c and [Pd(en)(dppz)](NO3)2 d, with CT-DNA
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232794&req=5

Figure 7: Scatchard plots for binding of [Pd(en)(bpy)](NO3)2 a, [Pd(en)(phen)](NO3)2 b, [Pd(en)(dpq)](NO3)2 c and [Pd(en)(dppz)](NO3)2 d, with CT-DNA
Mentions: In another experiment, a fixed amount of DNA was titrated with varying amount of each metal complex. The concentration of each metal complex bound to DNA, [L]b, and the concentration of each free metal complex, [L]f , are calculated by using the relationship [L]b=ΔA[L]f/ΔAmax. Here [L]f = [L]t –[L]b where [L]t is the maximum concentration of each metal complex added to saturate all the binding sites of DNA and ν is the ratio of the concentration of bound metal complex to total [DNA]. Using these data (ν, [L]f ), the Scatchard plots were constructed for the interaction of each metal complex at the two temperatures 300 K and 310 K. The Scatchard plots are shown in Figure 7 for a-d complexes. These plots are curvilinear concave downwards, suggesting cooperative binding (26).

Bottom Line: EBr and Scatchard experiments for a-d complexes suggest efficient intercalative binding affinity to CT-DNA giving the order: d > c > b > a.Several binding and thermodynamic parameters are also described.The biological activity of these cationic and water soluble palladium complexes were tested against chronic myelogenous leukemia cell line, K562. b, c and d complexes show cytotoxic concentration (Cc50) values much lower than cisplatin.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Sistan & Baluchestan, Zahedan, Iran.

ABSTRACT
In depth interaction studies between calf thymus deoxyribonucleic acid (CT-DNA) and a series of four structurally relative palladium(II) complexes [Pd(en)(HB)](NO3)2 (a-d), where en is ethylenediamine and heterocyclic base (HB) is 2,2'-bipyridine (bpy, a); 1,10-phenanthroline (phen, b); dipyridoquinoxaline (dpq, c) and dipyridophenazine (dppz, d) (Figure 1), were performed. These studies have been investigated by utilizing the electronic absorption spectroscopy, fluorescence spectra and ethidium bromide (EBr) displacement and gel filtration techniques. a-d complexes cooperatively bind and denature the DNA at low concentrations. Their concentration at midpoint of transition, L1/2, follows the order a > b > c > d. Also the g, the number of binding sites per 1000 nucleotides, follows the order a > b ~ c > d. EBr and Scatchard experiments for a-d complexes suggest efficient intercalative binding affinity to CT-DNA giving the order: d > c > b > a. Several binding and thermodynamic parameters are also described. The biological activity of these cationic and water soluble palladium complexes were tested against chronic myelogenous leukemia cell line, K562. b, c and d complexes show cytotoxic concentration (Cc50) values much lower than cisplatin.

No MeSH data available.


Related in: MedlinePlus