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The Effects of Extending of Co-planarity in a Series of Structurally Relative Polypyridyl Palladium(II) Complexes on DNA-binding and Cytotoxicity Properties.

Shahraki S, Mansouri-Torshizi H, Sori Nezami Z, Ghahghaei A, Yaghoubi F, Divsalar A, Saboury AA, H Shirazi F - Iran J Pharm Res (2014)

Bottom Line: EBr and Scatchard experiments for a-d complexes suggest efficient intercalative binding affinity to CT-DNA giving the order: d > c > b > a.Several binding and thermodynamic parameters are also described.The biological activity of these cationic and water soluble palladium complexes were tested against chronic myelogenous leukemia cell line, K562. b, c and d complexes show cytotoxic concentration (Cc50) values much lower than cisplatin.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Sistan & Baluchestan, Zahedan, Iran.

ABSTRACT
In depth interaction studies between calf thymus deoxyribonucleic acid (CT-DNA) and a series of four structurally relative palladium(II) complexes [Pd(en)(HB)](NO3)2 (a-d), where en is ethylenediamine and heterocyclic base (HB) is 2,2'-bipyridine (bpy, a); 1,10-phenanthroline (phen, b); dipyridoquinoxaline (dpq, c) and dipyridophenazine (dppz, d) (Figure 1), were performed. These studies have been investigated by utilizing the electronic absorption spectroscopy, fluorescence spectra and ethidium bromide (EBr) displacement and gel filtration techniques. a-d complexes cooperatively bind and denature the DNA at low concentrations. Their concentration at midpoint of transition, L1/2, follows the order a > b > c > d. Also the g, the number of binding sites per 1000 nucleotides, follows the order a > b ~ c > d. EBr and Scatchard experiments for a-d complexes suggest efficient intercalative binding affinity to CT-DNA giving the order: d > c > b > a. Several binding and thermodynamic parameters are also described. The biological activity of these cationic and water soluble palladium complexes were tested against chronic myelogenous leukemia cell line, K562. b, c and d complexes show cytotoxic concentration (Cc50) values much lower than cisplatin.

No MeSH data available.


Related in: MedlinePlus

The changes in the absorbance of fixed amount of metal complexes in the interaction with varying amount of CT-DNA at 300 K and 310 K. The linear plot of the reciprocal of ∆A vs the reciprocal of [DNA] for [Pd(en)(bpy)](NO3)2 a, [Pd(en)(phen)](NO3)2 b, [Pd(en)(dpq)](NO3)2 c and [Pd(en)(dppz)](NO3)2 d complexes.
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Figure 6: The changes in the absorbance of fixed amount of metal complexes in the interaction with varying amount of CT-DNA at 300 K and 310 K. The linear plot of the reciprocal of ∆A vs the reciprocal of [DNA] for [Pd(en)(bpy)](NO3)2 a, [Pd(en)(phen)](NO3)2 b, [Pd(en)(dpq)](NO3)2 c and [Pd(en)(dppz)](NO3)2 d complexes.

Mentions: A fixed amount of each metal complex was titrated with increasing concentration of DNA in total volume of 2 mL at 300 K and 310 K, separately. In this experiment, change in absorbance, ΔA, was calculated by subtracting the absorbance reading of mixed solutions of each metal complex with various concentrations of DNA, from absorbance reading of free metal complex. The values of ΔAmax, change in absorbance when all binding sites on DNA were occupied by each metal complex, are given in Table 3 and Figure 6.


The Effects of Extending of Co-planarity in a Series of Structurally Relative Polypyridyl Palladium(II) Complexes on DNA-binding and Cytotoxicity Properties.

Shahraki S, Mansouri-Torshizi H, Sori Nezami Z, Ghahghaei A, Yaghoubi F, Divsalar A, Saboury AA, H Shirazi F - Iran J Pharm Res (2014)

The changes in the absorbance of fixed amount of metal complexes in the interaction with varying amount of CT-DNA at 300 K and 310 K. The linear plot of the reciprocal of ∆A vs the reciprocal of [DNA] for [Pd(en)(bpy)](NO3)2 a, [Pd(en)(phen)](NO3)2 b, [Pd(en)(dpq)](NO3)2 c and [Pd(en)(dppz)](NO3)2 d complexes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232794&req=5

Figure 6: The changes in the absorbance of fixed amount of metal complexes in the interaction with varying amount of CT-DNA at 300 K and 310 K. The linear plot of the reciprocal of ∆A vs the reciprocal of [DNA] for [Pd(en)(bpy)](NO3)2 a, [Pd(en)(phen)](NO3)2 b, [Pd(en)(dpq)](NO3)2 c and [Pd(en)(dppz)](NO3)2 d complexes.
Mentions: A fixed amount of each metal complex was titrated with increasing concentration of DNA in total volume of 2 mL at 300 K and 310 K, separately. In this experiment, change in absorbance, ΔA, was calculated by subtracting the absorbance reading of mixed solutions of each metal complex with various concentrations of DNA, from absorbance reading of free metal complex. The values of ΔAmax, change in absorbance when all binding sites on DNA were occupied by each metal complex, are given in Table 3 and Figure 6.

Bottom Line: EBr and Scatchard experiments for a-d complexes suggest efficient intercalative binding affinity to CT-DNA giving the order: d > c > b > a.Several binding and thermodynamic parameters are also described.The biological activity of these cationic and water soluble palladium complexes were tested against chronic myelogenous leukemia cell line, K562. b, c and d complexes show cytotoxic concentration (Cc50) values much lower than cisplatin.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of Sistan & Baluchestan, Zahedan, Iran.

ABSTRACT
In depth interaction studies between calf thymus deoxyribonucleic acid (CT-DNA) and a series of four structurally relative palladium(II) complexes [Pd(en)(HB)](NO3)2 (a-d), where en is ethylenediamine and heterocyclic base (HB) is 2,2'-bipyridine (bpy, a); 1,10-phenanthroline (phen, b); dipyridoquinoxaline (dpq, c) and dipyridophenazine (dppz, d) (Figure 1), were performed. These studies have been investigated by utilizing the electronic absorption spectroscopy, fluorescence spectra and ethidium bromide (EBr) displacement and gel filtration techniques. a-d complexes cooperatively bind and denature the DNA at low concentrations. Their concentration at midpoint of transition, L1/2, follows the order a > b > c > d. Also the g, the number of binding sites per 1000 nucleotides, follows the order a > b ~ c > d. EBr and Scatchard experiments for a-d complexes suggest efficient intercalative binding affinity to CT-DNA giving the order: d > c > b > a. Several binding and thermodynamic parameters are also described. The biological activity of these cationic and water soluble palladium complexes were tested against chronic myelogenous leukemia cell line, K562. b, c and d complexes show cytotoxic concentration (Cc50) values much lower than cisplatin.

No MeSH data available.


Related in: MedlinePlus