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Study on the Multi-marker Components Quantitative HPLC Fingerprint of the Compound Chinese Medicine Wuwei Changyanning Granule.

Yang X, Yang SP, Zhang X, Yu XD, He QY, Wang BC - Iran J Pharm Res (2014)

Bottom Line: In addition, common peaks number, the percentage of non-common peaks and similarity were also studied.Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang.The results showed that the identification method of fingerprinting was reliable.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Chongqing Normal University, Chongqing 401331, P.R.China. ; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
The aim of this paper is to develop a rapid and highly sensitive quantitative HPLC fingerprint method with multiple indicators by using the Compound Chinese Medicine Wuwei Changyanning granule and 5 herbs in the prescription. The quantitative fingerprint chromatogram with multiple indicators was investigated. і)6 compositions included rutin, gallic acid, chlorogenic acid, atractylenolide Ⅰ, pachymic acid and apigenin, which originated from 5 herbs respectively, were selected as quantitative compositions, and their contents were determined using HPLC from 11 batches granules and the corresponding 5 medicinal materials. ⅱ) The precision, stability and repeatability of fingerprinting were investigated. In addition, common peaks number, the percentage of non-common peaks and similarity were also studied. Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang. The results showed that the identification method of fingerprinting was reliable.

No MeSH data available.


Percentage of unmutual peak area in the total peak area about 11 batches of granules and 5 raw herbs.
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Figure 11: Percentage of unmutual peak area in the total peak area about 11 batches of granules and 5 raw herbs.

Mentions: Besides the common peaks, there are non-common peaks in each chromatogram, which represents the fuzziness among the same kind of TCM. The percentages of non-common peaks in granules, Niuerfeng, Laliao, Baishu, Fuling and Guanghuoxiang were 6.72% -9.26%, 6.57%-9.01%, 5.88%-8.22%, 3.98%-5.34%, 3.27% -4.44% and 5.70% -6.32%, respectively. They are less than the national standard of 10 % (see Figure 11.).


Study on the Multi-marker Components Quantitative HPLC Fingerprint of the Compound Chinese Medicine Wuwei Changyanning Granule.

Yang X, Yang SP, Zhang X, Yu XD, He QY, Wang BC - Iran J Pharm Res (2014)

Percentage of unmutual peak area in the total peak area about 11 batches of granules and 5 raw herbs.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232784&req=5

Figure 11: Percentage of unmutual peak area in the total peak area about 11 batches of granules and 5 raw herbs.
Mentions: Besides the common peaks, there are non-common peaks in each chromatogram, which represents the fuzziness among the same kind of TCM. The percentages of non-common peaks in granules, Niuerfeng, Laliao, Baishu, Fuling and Guanghuoxiang were 6.72% -9.26%, 6.57%-9.01%, 5.88%-8.22%, 3.98%-5.34%, 3.27% -4.44% and 5.70% -6.32%, respectively. They are less than the national standard of 10 % (see Figure 11.).

Bottom Line: In addition, common peaks number, the percentage of non-common peaks and similarity were also studied.Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang.The results showed that the identification method of fingerprinting was reliable.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Chongqing Normal University, Chongqing 401331, P.R.China. ; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
The aim of this paper is to develop a rapid and highly sensitive quantitative HPLC fingerprint method with multiple indicators by using the Compound Chinese Medicine Wuwei Changyanning granule and 5 herbs in the prescription. The quantitative fingerprint chromatogram with multiple indicators was investigated. і)6 compositions included rutin, gallic acid, chlorogenic acid, atractylenolide Ⅰ, pachymic acid and apigenin, which originated from 5 herbs respectively, were selected as quantitative compositions, and their contents were determined using HPLC from 11 batches granules and the corresponding 5 medicinal materials. ⅱ) The precision, stability and repeatability of fingerprinting were investigated. In addition, common peaks number, the percentage of non-common peaks and similarity were also studied. Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang. The results showed that the identification method of fingerprinting was reliable.

No MeSH data available.