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Study on the Multi-marker Components Quantitative HPLC Fingerprint of the Compound Chinese Medicine Wuwei Changyanning Granule.

Yang X, Yang SP, Zhang X, Yu XD, He QY, Wang BC - Iran J Pharm Res (2014)

Bottom Line: In addition, common peaks number, the percentage of non-common peaks and similarity were also studied.Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang.The results showed that the identification method of fingerprinting was reliable.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Chongqing Normal University, Chongqing 401331, P.R.China. ; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
The aim of this paper is to develop a rapid and highly sensitive quantitative HPLC fingerprint method with multiple indicators by using the Compound Chinese Medicine Wuwei Changyanning granule and 5 herbs in the prescription. The quantitative fingerprint chromatogram with multiple indicators was investigated. і)6 compositions included rutin, gallic acid, chlorogenic acid, atractylenolide Ⅰ, pachymic acid and apigenin, which originated from 5 herbs respectively, were selected as quantitative compositions, and their contents were determined using HPLC from 11 batches granules and the corresponding 5 medicinal materials. ⅱ) The precision, stability and repeatability of fingerprinting were investigated. In addition, common peaks number, the percentage of non-common peaks and similarity were also studied. Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang. The results showed that the identification method of fingerprinting was reliable.

No MeSH data available.


Peak alignment of fingerprints of Pogostemon cablin(Blanco)Benth
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Figure 10: Peak alignment of fingerprints of Pogostemon cablin(Blanco)Benth

Mentions: According to the definition of fingerprints of TCM, a chromatographic fingerprint is in practice a chromatographic pattern of some common kinds of pharmacologically active and chemically characteristic components in the TCM. This chromatographic profile should be characterized by the fundamental attributions of “sameness” and “differences”. It is suggested that the authentication and identification of herbal medicines can be accurately conducted by chromatographic fingerprints. The chromatographic fingerprints could demonstrate both the “sameness” and “differences” between various samples successfully (13). With HPLC method, 11 batches of samples from different factories in China were analyzed in the optimum conditions. The average chromatogram from the 11 batches was regarded as the standardized characteristic fingerprint of WWCYNG. Peaks existed in all chromatograms of 11 samples were assigned as “common peaks”, indicating the sameness among various samples. The chromatograms of WWCYNG from the 11 samples consisted of 21 common peaks within 88 min, shown in Figure 5. Among these components, rutin was detected as a high and stable content, therefore it was chosen as the reference substance. All common peaks’ relative retention time and relative peak area were obtained with reference to this substance. R.S.D. values of the relative retention time of 21 common peaks in 11 batches samples were less than 1.0% which means the common peaks were in good correspondence in all samples (data not shown). Moreover, such low R.S.D. values demonstrated that the fingerprint by HPLC had good stability and reproducibility. So, the fingerprint of WWCYNG was composed of the peak profiles of the 21 components. Likewise, as shown from Figure 6 to Figure 10,the common peaks of 11 batches about Niuerfeng, Laliao, Baishu, Fuling and Guanghuoxiang, were 19, 15, 12, 7 and 13, respectively.


Study on the Multi-marker Components Quantitative HPLC Fingerprint of the Compound Chinese Medicine Wuwei Changyanning Granule.

Yang X, Yang SP, Zhang X, Yu XD, He QY, Wang BC - Iran J Pharm Res (2014)

Peak alignment of fingerprints of Pogostemon cablin(Blanco)Benth
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232784&req=5

Figure 10: Peak alignment of fingerprints of Pogostemon cablin(Blanco)Benth
Mentions: According to the definition of fingerprints of TCM, a chromatographic fingerprint is in practice a chromatographic pattern of some common kinds of pharmacologically active and chemically characteristic components in the TCM. This chromatographic profile should be characterized by the fundamental attributions of “sameness” and “differences”. It is suggested that the authentication and identification of herbal medicines can be accurately conducted by chromatographic fingerprints. The chromatographic fingerprints could demonstrate both the “sameness” and “differences” between various samples successfully (13). With HPLC method, 11 batches of samples from different factories in China were analyzed in the optimum conditions. The average chromatogram from the 11 batches was regarded as the standardized characteristic fingerprint of WWCYNG. Peaks existed in all chromatograms of 11 samples were assigned as “common peaks”, indicating the sameness among various samples. The chromatograms of WWCYNG from the 11 samples consisted of 21 common peaks within 88 min, shown in Figure 5. Among these components, rutin was detected as a high and stable content, therefore it was chosen as the reference substance. All common peaks’ relative retention time and relative peak area were obtained with reference to this substance. R.S.D. values of the relative retention time of 21 common peaks in 11 batches samples were less than 1.0% which means the common peaks were in good correspondence in all samples (data not shown). Moreover, such low R.S.D. values demonstrated that the fingerprint by HPLC had good stability and reproducibility. So, the fingerprint of WWCYNG was composed of the peak profiles of the 21 components. Likewise, as shown from Figure 6 to Figure 10,the common peaks of 11 batches about Niuerfeng, Laliao, Baishu, Fuling and Guanghuoxiang, were 19, 15, 12, 7 and 13, respectively.

Bottom Line: In addition, common peaks number, the percentage of non-common peaks and similarity were also studied.Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang.The results showed that the identification method of fingerprinting was reliable.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Chongqing Normal University, Chongqing 401331, P.R.China. ; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
The aim of this paper is to develop a rapid and highly sensitive quantitative HPLC fingerprint method with multiple indicators by using the Compound Chinese Medicine Wuwei Changyanning granule and 5 herbs in the prescription. The quantitative fingerprint chromatogram with multiple indicators was investigated. і)6 compositions included rutin, gallic acid, chlorogenic acid, atractylenolide Ⅰ, pachymic acid and apigenin, which originated from 5 herbs respectively, were selected as quantitative compositions, and their contents were determined using HPLC from 11 batches granules and the corresponding 5 medicinal materials. ⅱ) The precision, stability and repeatability of fingerprinting were investigated. In addition, common peaks number, the percentage of non-common peaks and similarity were also studied. Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang. The results showed that the identification method of fingerprinting was reliable.

No MeSH data available.