Limits...
Study on the Multi-marker Components Quantitative HPLC Fingerprint of the Compound Chinese Medicine Wuwei Changyanning Granule.

Yang X, Yang SP, Zhang X, Yu XD, He QY, Wang BC - Iran J Pharm Res (2014)

Bottom Line: In addition, common peaks number, the percentage of non-common peaks and similarity were also studied.Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang.The results showed that the identification method of fingerprinting was reliable.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Chongqing Normal University, Chongqing 401331, P.R.China. ; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
The aim of this paper is to develop a rapid and highly sensitive quantitative HPLC fingerprint method with multiple indicators by using the Compound Chinese Medicine Wuwei Changyanning granule and 5 herbs in the prescription. The quantitative fingerprint chromatogram with multiple indicators was investigated. і)6 compositions included rutin, gallic acid, chlorogenic acid, atractylenolide Ⅰ, pachymic acid and apigenin, which originated from 5 herbs respectively, were selected as quantitative compositions, and their contents were determined using HPLC from 11 batches granules and the corresponding 5 medicinal materials. ⅱ) The precision, stability and repeatability of fingerprinting were investigated. In addition, common peaks number, the percentage of non-common peaks and similarity were also studied. Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang. The results showed that the identification method of fingerprinting was reliable.

No MeSH data available.


Related in: MedlinePlus

Chromatogram of granule and Chinese herbal medicine in 360 nm and 273 nm Wavelength Note: The number of 1, 3, 5, 7, 9, 11 and 13 were the chromatograms corresponding to mixed standard solution, WWCYNG, Niuerfeng, Laliao, Baishu, Fuling and Guanghuoxiang under 360 nm conditions, respectively. The number of 2, 4, 6, 8, 10 and 12 were the chromatograms corresponding to mixed standard solution, WWCYNG, Niuerfeng, Laliao, Baishu, Fuling and Guanghuoxiang under 273 nm conditions, respectively. The number of 1’, 2’, 3’, 4’, 5’and 6’ were the compents corresponding to Gallic acid (tR:5.337 min), Pachymic acid(tR:17.386 min), Chlorogenic acid (tR:20.314 min), Rutin (tR:40.439 min), Atractylenolide Ⅰ (tR:65.557 min) and Apigenin ( tR:69.453 min), respectively.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4232784&req=5

Figure 1: Chromatogram of granule and Chinese herbal medicine in 360 nm and 273 nm Wavelength Note: The number of 1, 3, 5, 7, 9, 11 and 13 were the chromatograms corresponding to mixed standard solution, WWCYNG, Niuerfeng, Laliao, Baishu, Fuling and Guanghuoxiang under 360 nm conditions, respectively. The number of 2, 4, 6, 8, 10 and 12 were the chromatograms corresponding to mixed standard solution, WWCYNG, Niuerfeng, Laliao, Baishu, Fuling and Guanghuoxiang under 273 nm conditions, respectively. The number of 1’, 2’, 3’, 4’, 5’and 6’ were the compents corresponding to Gallic acid (tR:5.337 min), Pachymic acid(tR:17.386 min), Chlorogenic acid (tR:20.314 min), Rutin (tR:40.439 min), Atractylenolide Ⅰ (tR:65.557 min) and Apigenin ( tR:69.453 min), respectively.


Study on the Multi-marker Components Quantitative HPLC Fingerprint of the Compound Chinese Medicine Wuwei Changyanning Granule.

Yang X, Yang SP, Zhang X, Yu XD, He QY, Wang BC - Iran J Pharm Res (2014)

Chromatogram of granule and Chinese herbal medicine in 360 nm and 273 nm Wavelength Note: The number of 1, 3, 5, 7, 9, 11 and 13 were the chromatograms corresponding to mixed standard solution, WWCYNG, Niuerfeng, Laliao, Baishu, Fuling and Guanghuoxiang under 360 nm conditions, respectively. The number of 2, 4, 6, 8, 10 and 12 were the chromatograms corresponding to mixed standard solution, WWCYNG, Niuerfeng, Laliao, Baishu, Fuling and Guanghuoxiang under 273 nm conditions, respectively. The number of 1’, 2’, 3’, 4’, 5’and 6’ were the compents corresponding to Gallic acid (tR:5.337 min), Pachymic acid(tR:17.386 min), Chlorogenic acid (tR:20.314 min), Rutin (tR:40.439 min), Atractylenolide Ⅰ (tR:65.557 min) and Apigenin ( tR:69.453 min), respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232784&req=5

Figure 1: Chromatogram of granule and Chinese herbal medicine in 360 nm and 273 nm Wavelength Note: The number of 1, 3, 5, 7, 9, 11 and 13 were the chromatograms corresponding to mixed standard solution, WWCYNG, Niuerfeng, Laliao, Baishu, Fuling and Guanghuoxiang under 360 nm conditions, respectively. The number of 2, 4, 6, 8, 10 and 12 were the chromatograms corresponding to mixed standard solution, WWCYNG, Niuerfeng, Laliao, Baishu, Fuling and Guanghuoxiang under 273 nm conditions, respectively. The number of 1’, 2’, 3’, 4’, 5’and 6’ were the compents corresponding to Gallic acid (tR:5.337 min), Pachymic acid(tR:17.386 min), Chlorogenic acid (tR:20.314 min), Rutin (tR:40.439 min), Atractylenolide Ⅰ (tR:65.557 min) and Apigenin ( tR:69.453 min), respectively.
Bottom Line: In addition, common peaks number, the percentage of non-common peaks and similarity were also studied.Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang.The results showed that the identification method of fingerprinting was reliable.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Chongqing Normal University, Chongqing 401331, P.R.China. ; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
The aim of this paper is to develop a rapid and highly sensitive quantitative HPLC fingerprint method with multiple indicators by using the Compound Chinese Medicine Wuwei Changyanning granule and 5 herbs in the prescription. The quantitative fingerprint chromatogram with multiple indicators was investigated. і)6 compositions included rutin, gallic acid, chlorogenic acid, atractylenolide Ⅰ, pachymic acid and apigenin, which originated from 5 herbs respectively, were selected as quantitative compositions, and their contents were determined using HPLC from 11 batches granules and the corresponding 5 medicinal materials. ⅱ) The precision, stability and repeatability of fingerprinting were investigated. In addition, common peaks number, the percentage of non-common peaks and similarity were also studied. Among them, 21 common peaks in the granule could find the source of peaks from the 5 herbs, among of 10 peaks from Niuerfeng, 9 peaks from Laliao, 3 peaks from Baishu, 3 peaks from Fuling and 5 peaks from Guanghuoxiang. The results showed that the identification method of fingerprinting was reliable.

No MeSH data available.


Related in: MedlinePlus