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Effect of zirconium dioxide nanoparticles on glutathione peroxidase enzyme in PC12 and n2a cell lines.

Asadpour E, Sadeghnia HR, Ghorbani A, Boroushaki MT - Iran J Pharm Res (2014)

Bottom Line: Nano-ZrO2 caused a significant reduction in cell viability and GPx activity after 12, 24 and 48 h, as compared with control group.These effects were concentration dependent and started from 250 µg/mL.The present study demonstrated that nano-ZrO2, at concentrations of > 250 µg/mL, has antiproliferative effects via reducing the cell defense mechanism against oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT
Today, special attention is paid to the use of zirconium dioxide nanoparticle (nano-ZrO2), a neutral bioceramic metal, particularly for drug and gene delivery in medicine. However, there are some reports implying that use of nano-ZrO2 is associated with cytotoxic effects like inhibiting the cell proliferation, DNA damage and apoptosis. In the present study, we examined whether nano-ZrO2 alters cell viability and glutathione peroxidase (GPx) activity in two neuronal cell lines. The PC12 and N2a cells were cultured in the absence or presence of varying concentrations (31.25-2000 µg/mL) of nano-ZrO2 for 12, 24 or 48 h. The cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and GPx activity was determined by quantifying the rate of oxidation of the reduced glutathione to the oxidized glutathione. Nano-ZrO2 caused a significant reduction in cell viability and GPx activity after 12, 24 and 48 h, as compared with control group. These effects were concentration dependent and started from 250 µg/mL. The present study demonstrated that nano-ZrO2, at concentrations of > 250 µg/mL, has antiproliferative effects via reducing the cell defense mechanism against oxidative stress.

No MeSH data available.


Related in: MedlinePlus

Effects of nano-ZrO2 on glutathione peroxidase activity (GPx), in A: N2a cell line, B: PCl2 cell line. Cells were treated with different concentrations of zirconia for 24 hours. The GPx concentration is normalized to the protein concentration and the activity of enzyme was expressed as unit/mg protein. One way ANOVA analysis show significant difference between the data which were investigated *p<0.05 **p<0.01 and ***p<0.001, as compared with control group
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Figure 5: Effects of nano-ZrO2 on glutathione peroxidase activity (GPx), in A: N2a cell line, B: PCl2 cell line. Cells were treated with different concentrations of zirconia for 24 hours. The GPx concentration is normalized to the protein concentration and the activity of enzyme was expressed as unit/mg protein. One way ANOVA analysis show significant difference between the data which were investigated *p<0.05 **p<0.01 and ***p<0.001, as compared with control group

Mentions: The influence of nano-ZrO2 on GPx enzyme is determined with decreasing its activity comparing to the untreated group which this reduction is started from 250 µg/mL (p < 0.001) after 12 h exposure of N2a cell line (Figure 4A) and it is distributed to lower concentration via rising the duration of exposure time up to 24 h and 48 h which are 62.5 µg/mL and 31.25 µg/mL (p < 0.05) (Figure 5A and 6A), respectively.


Effect of zirconium dioxide nanoparticles on glutathione peroxidase enzyme in PC12 and n2a cell lines.

Asadpour E, Sadeghnia HR, Ghorbani A, Boroushaki MT - Iran J Pharm Res (2014)

Effects of nano-ZrO2 on glutathione peroxidase activity (GPx), in A: N2a cell line, B: PCl2 cell line. Cells were treated with different concentrations of zirconia for 24 hours. The GPx concentration is normalized to the protein concentration and the activity of enzyme was expressed as unit/mg protein. One way ANOVA analysis show significant difference between the data which were investigated *p<0.05 **p<0.01 and ***p<0.001, as compared with control group
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232778&req=5

Figure 5: Effects of nano-ZrO2 on glutathione peroxidase activity (GPx), in A: N2a cell line, B: PCl2 cell line. Cells were treated with different concentrations of zirconia for 24 hours. The GPx concentration is normalized to the protein concentration and the activity of enzyme was expressed as unit/mg protein. One way ANOVA analysis show significant difference between the data which were investigated *p<0.05 **p<0.01 and ***p<0.001, as compared with control group
Mentions: The influence of nano-ZrO2 on GPx enzyme is determined with decreasing its activity comparing to the untreated group which this reduction is started from 250 µg/mL (p < 0.001) after 12 h exposure of N2a cell line (Figure 4A) and it is distributed to lower concentration via rising the duration of exposure time up to 24 h and 48 h which are 62.5 µg/mL and 31.25 µg/mL (p < 0.05) (Figure 5A and 6A), respectively.

Bottom Line: Nano-ZrO2 caused a significant reduction in cell viability and GPx activity after 12, 24 and 48 h, as compared with control group.These effects were concentration dependent and started from 250 µg/mL.The present study demonstrated that nano-ZrO2, at concentrations of > 250 µg/mL, has antiproliferative effects via reducing the cell defense mechanism against oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT
Today, special attention is paid to the use of zirconium dioxide nanoparticle (nano-ZrO2), a neutral bioceramic metal, particularly for drug and gene delivery in medicine. However, there are some reports implying that use of nano-ZrO2 is associated with cytotoxic effects like inhibiting the cell proliferation, DNA damage and apoptosis. In the present study, we examined whether nano-ZrO2 alters cell viability and glutathione peroxidase (GPx) activity in two neuronal cell lines. The PC12 and N2a cells were cultured in the absence or presence of varying concentrations (31.25-2000 µg/mL) of nano-ZrO2 for 12, 24 or 48 h. The cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and GPx activity was determined by quantifying the rate of oxidation of the reduced glutathione to the oxidized glutathione. Nano-ZrO2 caused a significant reduction in cell viability and GPx activity after 12, 24 and 48 h, as compared with control group. These effects were concentration dependent and started from 250 µg/mL. The present study demonstrated that nano-ZrO2, at concentrations of > 250 µg/mL, has antiproliferative effects via reducing the cell defense mechanism against oxidative stress.

No MeSH data available.


Related in: MedlinePlus