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Effect of zirconium dioxide nanoparticles on glutathione peroxidase enzyme in PC12 and n2a cell lines.

Asadpour E, Sadeghnia HR, Ghorbani A, Boroushaki MT - Iran J Pharm Res (2014)

Bottom Line: Nano-ZrO2 caused a significant reduction in cell viability and GPx activity after 12, 24 and 48 h, as compared with control group.These effects were concentration dependent and started from 250 µg/mL.The present study demonstrated that nano-ZrO2, at concentrations of > 250 µg/mL, has antiproliferative effects via reducing the cell defense mechanism against oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT
Today, special attention is paid to the use of zirconium dioxide nanoparticle (nano-ZrO2), a neutral bioceramic metal, particularly for drug and gene delivery in medicine. However, there are some reports implying that use of nano-ZrO2 is associated with cytotoxic effects like inhibiting the cell proliferation, DNA damage and apoptosis. In the present study, we examined whether nano-ZrO2 alters cell viability and glutathione peroxidase (GPx) activity in two neuronal cell lines. The PC12 and N2a cells were cultured in the absence or presence of varying concentrations (31.25-2000 µg/mL) of nano-ZrO2 for 12, 24 or 48 h. The cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and GPx activity was determined by quantifying the rate of oxidation of the reduced glutathione to the oxidized glutathione. Nano-ZrO2 caused a significant reduction in cell viability and GPx activity after 12, 24 and 48 h, as compared with control group. These effects were concentration dependent and started from 250 µg/mL. The present study demonstrated that nano-ZrO2, at concentrations of > 250 µg/mL, has antiproliferative effects via reducing the cell defense mechanism against oxidative stress.

No MeSH data available.


Related in: MedlinePlus

Effects of nano-ZrO2 on N2a cell viability. Cells were treated with increasing concentrations of zirconia for 12, 24 and 48 h. The cell viability (quantified by MTS assay) is shown and discussed as percentage of control group (zirconia 0 µg/mL). Mean and SEM of three independent experiments are shown. One way ANOVA analysis shows significant difference between the data which were investigated in each time. ***p<0.001 versus all concentration more than 15.6 µg/mL in all 3 exposure time and *P<0.05 versus control group
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Figure 2: Effects of nano-ZrO2 on N2a cell viability. Cells were treated with increasing concentrations of zirconia for 12, 24 and 48 h. The cell viability (quantified by MTS assay) is shown and discussed as percentage of control group (zirconia 0 µg/mL). Mean and SEM of three independent experiments are shown. One way ANOVA analysis shows significant difference between the data which were investigated in each time. ***p<0.001 versus all concentration more than 15.6 µg/mL in all 3 exposure time and *P<0.05 versus control group

Mentions: The effect of nano-ZrO2 on viability of N2a cells are shown in Figure 2. After 12 h incubation, cell viability was significantly decreased at concentrations of ≥15.6 µg/mL. As compared with untreated cells (100 ± 9.3 %), the nano-ZrO2 at 2000 µg/mL decreased the cell viability to 32.76 ± 2.8 (P < 0.001), which was lowest viability amount among all the concentrations. Similarly, exposure of the N2a cells to the nanoparticles for 24 and 48 h showed a concentration-dependent decrease in cell viability. Again the cytotoxic effect was observed at concentrations more than 15.6 µg/mL (P < 0.05 and P<0.001) after 24 and 48 h exposure, respectively. No significant difference in cell viability was found between the three incubation times.


Effect of zirconium dioxide nanoparticles on glutathione peroxidase enzyme in PC12 and n2a cell lines.

Asadpour E, Sadeghnia HR, Ghorbani A, Boroushaki MT - Iran J Pharm Res (2014)

Effects of nano-ZrO2 on N2a cell viability. Cells were treated with increasing concentrations of zirconia for 12, 24 and 48 h. The cell viability (quantified by MTS assay) is shown and discussed as percentage of control group (zirconia 0 µg/mL). Mean and SEM of three independent experiments are shown. One way ANOVA analysis shows significant difference between the data which were investigated in each time. ***p<0.001 versus all concentration more than 15.6 µg/mL in all 3 exposure time and *P<0.05 versus control group
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232778&req=5

Figure 2: Effects of nano-ZrO2 on N2a cell viability. Cells were treated with increasing concentrations of zirconia for 12, 24 and 48 h. The cell viability (quantified by MTS assay) is shown and discussed as percentage of control group (zirconia 0 µg/mL). Mean and SEM of three independent experiments are shown. One way ANOVA analysis shows significant difference between the data which were investigated in each time. ***p<0.001 versus all concentration more than 15.6 µg/mL in all 3 exposure time and *P<0.05 versus control group
Mentions: The effect of nano-ZrO2 on viability of N2a cells are shown in Figure 2. After 12 h incubation, cell viability was significantly decreased at concentrations of ≥15.6 µg/mL. As compared with untreated cells (100 ± 9.3 %), the nano-ZrO2 at 2000 µg/mL decreased the cell viability to 32.76 ± 2.8 (P < 0.001), which was lowest viability amount among all the concentrations. Similarly, exposure of the N2a cells to the nanoparticles for 24 and 48 h showed a concentration-dependent decrease in cell viability. Again the cytotoxic effect was observed at concentrations more than 15.6 µg/mL (P < 0.05 and P<0.001) after 24 and 48 h exposure, respectively. No significant difference in cell viability was found between the three incubation times.

Bottom Line: Nano-ZrO2 caused a significant reduction in cell viability and GPx activity after 12, 24 and 48 h, as compared with control group.These effects were concentration dependent and started from 250 µg/mL.The present study demonstrated that nano-ZrO2, at concentrations of > 250 µg/mL, has antiproliferative effects via reducing the cell defense mechanism against oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT
Today, special attention is paid to the use of zirconium dioxide nanoparticle (nano-ZrO2), a neutral bioceramic metal, particularly for drug and gene delivery in medicine. However, there are some reports implying that use of nano-ZrO2 is associated with cytotoxic effects like inhibiting the cell proliferation, DNA damage and apoptosis. In the present study, we examined whether nano-ZrO2 alters cell viability and glutathione peroxidase (GPx) activity in two neuronal cell lines. The PC12 and N2a cells were cultured in the absence or presence of varying concentrations (31.25-2000 µg/mL) of nano-ZrO2 for 12, 24 or 48 h. The cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and GPx activity was determined by quantifying the rate of oxidation of the reduced glutathione to the oxidized glutathione. Nano-ZrO2 caused a significant reduction in cell viability and GPx activity after 12, 24 and 48 h, as compared with control group. These effects were concentration dependent and started from 250 µg/mL. The present study demonstrated that nano-ZrO2, at concentrations of > 250 µg/mL, has antiproliferative effects via reducing the cell defense mechanism against oxidative stress.

No MeSH data available.


Related in: MedlinePlus