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Near-isogenic lines of Triticum aestivum with distinct modes of resistance exhibit dissimilar transcriptional regulation during Diuraphis noxia feeding.

Botha AM, van Eck L, Burger NF, Swanevelder ZH - Biol Open (2014)

Bottom Line: Although the effects of several D. noxia (Dn) resistance genes are known, none have been isolated or characterized.Tolerance is a passive resistance mechanism that acts through repair or de novo synthesis of photosystem proteins.Results further suggest that ethylene-mediated pathways are possibly involved in generating volatile compounds and cell wall fortification during the antixenosic response.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Stellenbosch University, Stellenbosch 7601, South Africa ambo@sun.ac.za.

No MeSH data available.


Related in: MedlinePlus

D. noxia feeding results in peroxide accumulation at aphid feeding sites after staining leaves of infested near isogenic wheat lines plants with 3,3′-diaminobenzidine (DAB) 132 hpi.Leaves are representative of five independent biological replicates per treatment. (A) Tugela; (B) Tugela-Dn1 (antibiotic); (C) Tugela-Dn2 (tolerant); (D) Tugela-Dn5 (antixenotic). Tugela-Dn1 (B) and Tugela-Dn5 (D) show dark areas around aphid feeding sites indicating the presence of peroxide. Tugela (A) and Tugela-Dn2 (C) only show dark areas at cut ends of the leaves.
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f06: D. noxia feeding results in peroxide accumulation at aphid feeding sites after staining leaves of infested near isogenic wheat lines plants with 3,3′-diaminobenzidine (DAB) 132 hpi.Leaves are representative of five independent biological replicates per treatment. (A) Tugela; (B) Tugela-Dn1 (antibiotic); (C) Tugela-Dn2 (tolerant); (D) Tugela-Dn5 (antixenotic). Tugela-Dn1 (B) and Tugela-Dn5 (D) show dark areas around aphid feeding sites indicating the presence of peroxide. Tugela (A) and Tugela-Dn2 (C) only show dark areas at cut ends of the leaves.

Mentions: To corroborate the changes in the oxidative burst in the NILs in response to D. noxia biotype SA1 feeding, leaves were collected and stained for H2O2 using 3,3′-diaminobenzidine (DAB), which forms reddish-brown polymerized deposits in the presence of peroxidase (Fig. 6). Dark staining was observed in Tugela-Dn1 and Tugela-Dn5 around the feeding sites (Fig. 6B and Fig. 5D), but staining was only visible around the regions where the leaves were cut in Tugela and Tugela-Dn2 (Fig. 6A,C). This indicates that the generation of aphid-induced ROS was active only in the NILs associated with antibiotic and antixenotic resistance responses, but not the susceptible Tugela nor the tolerant Tugela-Dn2.


Near-isogenic lines of Triticum aestivum with distinct modes of resistance exhibit dissimilar transcriptional regulation during Diuraphis noxia feeding.

Botha AM, van Eck L, Burger NF, Swanevelder ZH - Biol Open (2014)

D. noxia feeding results in peroxide accumulation at aphid feeding sites after staining leaves of infested near isogenic wheat lines plants with 3,3′-diaminobenzidine (DAB) 132 hpi.Leaves are representative of five independent biological replicates per treatment. (A) Tugela; (B) Tugela-Dn1 (antibiotic); (C) Tugela-Dn2 (tolerant); (D) Tugela-Dn5 (antixenotic). Tugela-Dn1 (B) and Tugela-Dn5 (D) show dark areas around aphid feeding sites indicating the presence of peroxide. Tugela (A) and Tugela-Dn2 (C) only show dark areas at cut ends of the leaves.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232770&req=5

f06: D. noxia feeding results in peroxide accumulation at aphid feeding sites after staining leaves of infested near isogenic wheat lines plants with 3,3′-diaminobenzidine (DAB) 132 hpi.Leaves are representative of five independent biological replicates per treatment. (A) Tugela; (B) Tugela-Dn1 (antibiotic); (C) Tugela-Dn2 (tolerant); (D) Tugela-Dn5 (antixenotic). Tugela-Dn1 (B) and Tugela-Dn5 (D) show dark areas around aphid feeding sites indicating the presence of peroxide. Tugela (A) and Tugela-Dn2 (C) only show dark areas at cut ends of the leaves.
Mentions: To corroborate the changes in the oxidative burst in the NILs in response to D. noxia biotype SA1 feeding, leaves were collected and stained for H2O2 using 3,3′-diaminobenzidine (DAB), which forms reddish-brown polymerized deposits in the presence of peroxidase (Fig. 6). Dark staining was observed in Tugela-Dn1 and Tugela-Dn5 around the feeding sites (Fig. 6B and Fig. 5D), but staining was only visible around the regions where the leaves were cut in Tugela and Tugela-Dn2 (Fig. 6A,C). This indicates that the generation of aphid-induced ROS was active only in the NILs associated with antibiotic and antixenotic resistance responses, but not the susceptible Tugela nor the tolerant Tugela-Dn2.

Bottom Line: Although the effects of several D. noxia (Dn) resistance genes are known, none have been isolated or characterized.Tolerance is a passive resistance mechanism that acts through repair or de novo synthesis of photosystem proteins.Results further suggest that ethylene-mediated pathways are possibly involved in generating volatile compounds and cell wall fortification during the antixenosic response.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetics, Stellenbosch University, Stellenbosch 7601, South Africa ambo@sun.ac.za.

No MeSH data available.


Related in: MedlinePlus