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Asymmetric inheritance of cytoophidia in Schizosaccharomyces pombe.

Zhang J, Hulme L, Liu JL - Biol Open (2014)

Bottom Line: Our time-lapse studies suggest that cytoophidia are dynamic.Once the mother cell divides, the cytoplasmic and nuclear cytoophidia independently partition into one of the two daughter cells.Our findings on asymmetric inheritance of cytoophidia in S. pombe offer an exciting opportunity to study the inheritance of metabolic enzymes in a well-studied model system.

View Article: PubMed Central - PubMed

Affiliation: MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3PT, UK.

No MeSH data available.


Related in: MedlinePlus

De novo formation of cytoophidia in S. pombe.Snapshots of a 4-hour live imaging recording a cell undergoing cell division. CTP synthase (CTPS) fused to YFP was expressed under the endogenous promoter. At the start timepoint (00:00:00), the mother cell clearly shows one cytoplasmic cytoophidium (C-cytoophidium, red in the cartoons) and one nuclear cytoophidium (N-cytoophidium, green in the cartoons). During cell division (02:11:00), the daughter cell at the top inherits the cytoophidia. The cell at the bottom does not have cytoophidia for about 50 min until a cytoophidium forms de novo (03:04:00). At the timepoint 03:34:00, both C- and N-cytoophidia inside the cell at the bottom grow, similar to the cytoophidia inside the cell at the top. Scale bar: 10 µm.
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f06: De novo formation of cytoophidia in S. pombe.Snapshots of a 4-hour live imaging recording a cell undergoing cell division. CTP synthase (CTPS) fused to YFP was expressed under the endogenous promoter. At the start timepoint (00:00:00), the mother cell clearly shows one cytoplasmic cytoophidium (C-cytoophidium, red in the cartoons) and one nuclear cytoophidium (N-cytoophidium, green in the cartoons). During cell division (02:11:00), the daughter cell at the top inherits the cytoophidia. The cell at the bottom does not have cytoophidia for about 50 min until a cytoophidium forms de novo (03:04:00). At the timepoint 03:34:00, both C- and N-cytoophidia inside the cell at the bottom grow, similar to the cytoophidia inside the cell at the top. Scale bar: 10 µm.

Mentions: To better understand the behavior of cytoophidia, we performed live imaging with S. pombe cells expressing Cts1-YFP. When we recorded live cells every 5 sec, we found that N-cytoophidia appeared highly dynamic. The traces of N-cytoophidia indicate that they mostly stay at the periphery of the nucleus, raising the possibility that N-cytoophidia tether on the nuclear envelope. Traces of C-cytoophidia showed that they moved dynamically in the cytoplasm between one end and the central region when the nucleus resides. The nucleus appears to serve as a barrier which, in most cases, prevents the C-cytoophidium moving from one end towards another end of the cell (Fig. 5A–E; supplementary material Movies 1 and 2). To see if the confinement of C-cytoophidia at G2 phase affects cytoophidium during cell division, we performed live imaging of S. pombe cells processing from G2 to mitosis, cytokinesis and abscission. Indeed, we found that the location of cytoophidia at G2 phase correlated with the differential inheritance of cytoophidia (Fig. 5F,G; supplementary material Movie 3). Since we found that more than 90% S. pombe cells contain C-cytoophidia, we predicted that cytoophidia will form de novo in the daughter cell that does not inherit the C-cytoophidium from the mother cell. Our prediction was confirmed by long-period time-lapse studies (Fig. 6; supplementary material Movie 4).


Asymmetric inheritance of cytoophidia in Schizosaccharomyces pombe.

Zhang J, Hulme L, Liu JL - Biol Open (2014)

De novo formation of cytoophidia in S. pombe.Snapshots of a 4-hour live imaging recording a cell undergoing cell division. CTP synthase (CTPS) fused to YFP was expressed under the endogenous promoter. At the start timepoint (00:00:00), the mother cell clearly shows one cytoplasmic cytoophidium (C-cytoophidium, red in the cartoons) and one nuclear cytoophidium (N-cytoophidium, green in the cartoons). During cell division (02:11:00), the daughter cell at the top inherits the cytoophidia. The cell at the bottom does not have cytoophidia for about 50 min until a cytoophidium forms de novo (03:04:00). At the timepoint 03:34:00, both C- and N-cytoophidia inside the cell at the bottom grow, similar to the cytoophidia inside the cell at the top. Scale bar: 10 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232767&req=5

f06: De novo formation of cytoophidia in S. pombe.Snapshots of a 4-hour live imaging recording a cell undergoing cell division. CTP synthase (CTPS) fused to YFP was expressed under the endogenous promoter. At the start timepoint (00:00:00), the mother cell clearly shows one cytoplasmic cytoophidium (C-cytoophidium, red in the cartoons) and one nuclear cytoophidium (N-cytoophidium, green in the cartoons). During cell division (02:11:00), the daughter cell at the top inherits the cytoophidia. The cell at the bottom does not have cytoophidia for about 50 min until a cytoophidium forms de novo (03:04:00). At the timepoint 03:34:00, both C- and N-cytoophidia inside the cell at the bottom grow, similar to the cytoophidia inside the cell at the top. Scale bar: 10 µm.
Mentions: To better understand the behavior of cytoophidia, we performed live imaging with S. pombe cells expressing Cts1-YFP. When we recorded live cells every 5 sec, we found that N-cytoophidia appeared highly dynamic. The traces of N-cytoophidia indicate that they mostly stay at the periphery of the nucleus, raising the possibility that N-cytoophidia tether on the nuclear envelope. Traces of C-cytoophidia showed that they moved dynamically in the cytoplasm between one end and the central region when the nucleus resides. The nucleus appears to serve as a barrier which, in most cases, prevents the C-cytoophidium moving from one end towards another end of the cell (Fig. 5A–E; supplementary material Movies 1 and 2). To see if the confinement of C-cytoophidia at G2 phase affects cytoophidium during cell division, we performed live imaging of S. pombe cells processing from G2 to mitosis, cytokinesis and abscission. Indeed, we found that the location of cytoophidia at G2 phase correlated with the differential inheritance of cytoophidia (Fig. 5F,G; supplementary material Movie 3). Since we found that more than 90% S. pombe cells contain C-cytoophidia, we predicted that cytoophidia will form de novo in the daughter cell that does not inherit the C-cytoophidium from the mother cell. Our prediction was confirmed by long-period time-lapse studies (Fig. 6; supplementary material Movie 4).

Bottom Line: Our time-lapse studies suggest that cytoophidia are dynamic.Once the mother cell divides, the cytoplasmic and nuclear cytoophidia independently partition into one of the two daughter cells.Our findings on asymmetric inheritance of cytoophidia in S. pombe offer an exciting opportunity to study the inheritance of metabolic enzymes in a well-studied model system.

View Article: PubMed Central - PubMed

Affiliation: MRC Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3PT, UK.

No MeSH data available.


Related in: MedlinePlus