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In vitro co-culture systems for studying molecular basis of cellular interaction between Aire-expressing medullary thymic epithelial cells and fresh thymocytes.

Yamaguchi Y, Kudoh J, Yoshida T, Shimizu N - Biol Open (2014)

Bottom Line: Thus, these Aire(+) cells appear to behave like differentiating mTECs as if they pass through the developmental stages from intermediate state toward mature state.Surprisingly, an in vitro co-culture system consisting of Aire(+) cells and fractionated sub-populations of fresh thymocytes implied the possible existence of two distinct subtypes of thymocytes (named as CD4(+) killer and CD4(-) rescuer) that may determine the fate (dead or alive) of the differentiating Aire(+)mTECs.Thus, our in vitro co-culture system appears to mimic a part of "in vivo thymic crosstalk".

View Article: PubMed Central - PubMed

Affiliation: Advanced Research Center for Genome Super Power, Keio University, 2 Okubo, Tsukuba, Ibaraki 300-2611, Japan.

No MeSH data available.


Related in: MedlinePlus

Expression of several genes specific for tumor necrosis factor-receptor super families (TNFRSFs) with and without death domain in three Aire+ cell lines.The Tnfrsf-specific mRNA in total RNA was quantified by qRT-PCR analysis with primers specific for 26 different Tnfrsf members (Tnfrsf-1a, -1b, -3, -4, -5, -6, -7, -8, -9, 10b, -11a, -11b, -12a, -13b, -13c, -14, -16, -17, -18, -19, -21, -22, -23, -25, -26 and -27) and β-Actin. Values are arbitrary units (a.u.) normalized to β-Actin. Average of three separate experiments was shown with standard deviation (bar) above the mean of at least three independent experiments. Asterisk indicates eight members known to possess death domain (Tnfrsf-1a, -6, -10b, -11b, -12a, -16, -21, and -26).
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f05: Expression of several genes specific for tumor necrosis factor-receptor super families (TNFRSFs) with and without death domain in three Aire+ cell lines.The Tnfrsf-specific mRNA in total RNA was quantified by qRT-PCR analysis with primers specific for 26 different Tnfrsf members (Tnfrsf-1a, -1b, -3, -4, -5, -6, -7, -8, -9, 10b, -11a, -11b, -12a, -13b, -13c, -14, -16, -17, -18, -19, -21, -22, -23, -25, -26 and -27) and β-Actin. Values are arbitrary units (a.u.) normalized to β-Actin. Average of three separate experiments was shown with standard deviation (bar) above the mean of at least three independent experiments. Asterisk indicates eight members known to possess death domain (Tnfrsf-1a, -6, -10b, -11b, -12a, -16, -21, and -26).

Mentions: qRT-PCR analysis revealed that a number of TNFRSF members are expressed in all three Aire+ cell lines (Fig. 5). Most of 26 members tested were expressed at similar levels. However, expression of five members (Tnfrsf-8, -11a, -11b, -17, -21) was different between two cell types (Aire+TECs and Aire+DC). On the other hand, no difference was seen for other five members (Tnfrsf-1a, -6, -10b, -12a, and -16).


In vitro co-culture systems for studying molecular basis of cellular interaction between Aire-expressing medullary thymic epithelial cells and fresh thymocytes.

Yamaguchi Y, Kudoh J, Yoshida T, Shimizu N - Biol Open (2014)

Expression of several genes specific for tumor necrosis factor-receptor super families (TNFRSFs) with and without death domain in three Aire+ cell lines.The Tnfrsf-specific mRNA in total RNA was quantified by qRT-PCR analysis with primers specific for 26 different Tnfrsf members (Tnfrsf-1a, -1b, -3, -4, -5, -6, -7, -8, -9, 10b, -11a, -11b, -12a, -13b, -13c, -14, -16, -17, -18, -19, -21, -22, -23, -25, -26 and -27) and β-Actin. Values are arbitrary units (a.u.) normalized to β-Actin. Average of three separate experiments was shown with standard deviation (bar) above the mean of at least three independent experiments. Asterisk indicates eight members known to possess death domain (Tnfrsf-1a, -6, -10b, -11b, -12a, -16, -21, and -26).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232765&req=5

f05: Expression of several genes specific for tumor necrosis factor-receptor super families (TNFRSFs) with and without death domain in three Aire+ cell lines.The Tnfrsf-specific mRNA in total RNA was quantified by qRT-PCR analysis with primers specific for 26 different Tnfrsf members (Tnfrsf-1a, -1b, -3, -4, -5, -6, -7, -8, -9, 10b, -11a, -11b, -12a, -13b, -13c, -14, -16, -17, -18, -19, -21, -22, -23, -25, -26 and -27) and β-Actin. Values are arbitrary units (a.u.) normalized to β-Actin. Average of three separate experiments was shown with standard deviation (bar) above the mean of at least three independent experiments. Asterisk indicates eight members known to possess death domain (Tnfrsf-1a, -6, -10b, -11b, -12a, -16, -21, and -26).
Mentions: qRT-PCR analysis revealed that a number of TNFRSF members are expressed in all three Aire+ cell lines (Fig. 5). Most of 26 members tested were expressed at similar levels. However, expression of five members (Tnfrsf-8, -11a, -11b, -17, -21) was different between two cell types (Aire+TECs and Aire+DC). On the other hand, no difference was seen for other five members (Tnfrsf-1a, -6, -10b, -12a, and -16).

Bottom Line: Thus, these Aire(+) cells appear to behave like differentiating mTECs as if they pass through the developmental stages from intermediate state toward mature state.Surprisingly, an in vitro co-culture system consisting of Aire(+) cells and fractionated sub-populations of fresh thymocytes implied the possible existence of two distinct subtypes of thymocytes (named as CD4(+) killer and CD4(-) rescuer) that may determine the fate (dead or alive) of the differentiating Aire(+)mTECs.Thus, our in vitro co-culture system appears to mimic a part of "in vivo thymic crosstalk".

View Article: PubMed Central - PubMed

Affiliation: Advanced Research Center for Genome Super Power, Keio University, 2 Okubo, Tsukuba, Ibaraki 300-2611, Japan.

No MeSH data available.


Related in: MedlinePlus