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Insights into the skeletal muscle characteristics of three southern African antelope species.

Kohn TA - Biol Open (2014)

Bottom Line: Overall, all three species had high oxidative and glycolytic capacities, but species differences were found.This study confirmed large variation in oxidative capacities within a single fibre type, as well as overlap between the fibre types with no distinct differences between the three species.The fibre type profile of each species is discussed and confirms some of their physical attributes and capabilities.

View Article: PubMed Central - PubMed

Affiliation: UCT/MRC Research Unit for Exercise Science and Sports Medicine, Department of Human Biology, PO Box 115, Newlands 7725, South Africa.

No MeSH data available.


Histology of mountain reedbuck, blesbok and kudu Vastus lateralis muscles.Fibres were classified as types I (I), IIA (A), IIAX (AX) and IIX (X). Type IIX fibres presenting with high oxidative capacity are labelled as Xo. (A–C) ATPase stain at pH 10.3. Type I fibres are clear. All other fibres are stained dark. Immunohistochemistry using an antibody specific to: (D–F) MHC I (BAD5); (G–I) MHC I and IIa (BF35); (J–L) MHC IIx (6H1). Note cross-reactivity with type I muscle fibres in all three species. (M–O) NADH stain showing oxidative capacity of muscle fibres.
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f02: Histology of mountain reedbuck, blesbok and kudu Vastus lateralis muscles.Fibres were classified as types I (I), IIA (A), IIAX (AX) and IIX (X). Type IIX fibres presenting with high oxidative capacity are labelled as Xo. (A–C) ATPase stain at pH 10.3. Type I fibres are clear. All other fibres are stained dark. Immunohistochemistry using an antibody specific to: (D–F) MHC I (BAD5); (G–I) MHC I and IIa (BF35); (J–L) MHC IIx (6H1). Note cross-reactivity with type I muscle fibres in all three species. (M–O) NADH stain showing oxidative capacity of muscle fibres.

Mentions: The NADH-tetrazolium stain, ATPase stain at pH 10.3 and immunohistochemistry using antibodies specific to MHC I (BAD5), MHC I and MHC IIa (BF35), and MHC IIx (6H1) are presented in Fig. 2. BAD5 (Fig. 2D–F) only reacted with type I fibres and corresponded to pure type I fibres, corresponding to the type I profiles depicted in the ATPase stains (Fig. 2A–C). Fibres containing either pure MHC I or MHC IIa, both, or MHC IIa in combination with MHC IIx reacted with BF35 (Fig. 2G–I). The monoclonal 6H1 reacted with fibres containing MHC IIx, but also cross-reacted with pure type I fibres (Fig. 2J–L). Fig. 2M–O depicts the oxidative capacity of the different fibre types. Fibres containing MHC I primarily had darker staining intensities compared to fibres containing MHC IIa or IIx, indicating a greater oxidative capacity. The MHC IIb isoform could not be detected in these slides using the specific MHC IIb antibodies (BF-F3 and 10F5) (data not shown).


Insights into the skeletal muscle characteristics of three southern African antelope species.

Kohn TA - Biol Open (2014)

Histology of mountain reedbuck, blesbok and kudu Vastus lateralis muscles.Fibres were classified as types I (I), IIA (A), IIAX (AX) and IIX (X). Type IIX fibres presenting with high oxidative capacity are labelled as Xo. (A–C) ATPase stain at pH 10.3. Type I fibres are clear. All other fibres are stained dark. Immunohistochemistry using an antibody specific to: (D–F) MHC I (BAD5); (G–I) MHC I and IIa (BF35); (J–L) MHC IIx (6H1). Note cross-reactivity with type I muscle fibres in all three species. (M–O) NADH stain showing oxidative capacity of muscle fibres.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232761&req=5

f02: Histology of mountain reedbuck, blesbok and kudu Vastus lateralis muscles.Fibres were classified as types I (I), IIA (A), IIAX (AX) and IIX (X). Type IIX fibres presenting with high oxidative capacity are labelled as Xo. (A–C) ATPase stain at pH 10.3. Type I fibres are clear. All other fibres are stained dark. Immunohistochemistry using an antibody specific to: (D–F) MHC I (BAD5); (G–I) MHC I and IIa (BF35); (J–L) MHC IIx (6H1). Note cross-reactivity with type I muscle fibres in all three species. (M–O) NADH stain showing oxidative capacity of muscle fibres.
Mentions: The NADH-tetrazolium stain, ATPase stain at pH 10.3 and immunohistochemistry using antibodies specific to MHC I (BAD5), MHC I and MHC IIa (BF35), and MHC IIx (6H1) are presented in Fig. 2. BAD5 (Fig. 2D–F) only reacted with type I fibres and corresponded to pure type I fibres, corresponding to the type I profiles depicted in the ATPase stains (Fig. 2A–C). Fibres containing either pure MHC I or MHC IIa, both, or MHC IIa in combination with MHC IIx reacted with BF35 (Fig. 2G–I). The monoclonal 6H1 reacted with fibres containing MHC IIx, but also cross-reacted with pure type I fibres (Fig. 2J–L). Fig. 2M–O depicts the oxidative capacity of the different fibre types. Fibres containing MHC I primarily had darker staining intensities compared to fibres containing MHC IIa or IIx, indicating a greater oxidative capacity. The MHC IIb isoform could not be detected in these slides using the specific MHC IIb antibodies (BF-F3 and 10F5) (data not shown).

Bottom Line: Overall, all three species had high oxidative and glycolytic capacities, but species differences were found.This study confirmed large variation in oxidative capacities within a single fibre type, as well as overlap between the fibre types with no distinct differences between the three species.The fibre type profile of each species is discussed and confirms some of their physical attributes and capabilities.

View Article: PubMed Central - PubMed

Affiliation: UCT/MRC Research Unit for Exercise Science and Sports Medicine, Department of Human Biology, PO Box 115, Newlands 7725, South Africa.

No MeSH data available.