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Differential responsiveness of distinct retinal domains to Atoh7.

Sinn R, Peravali R, Heermann S, Wittbrodt J - Mech. Dev. (2014)

Bottom Line: Atoh7 loss of function results in a massive reduction or even a total loss of RGCs.Photoreceptors were largely absent and the Müller glia cell number was reduced significantly.The majority of cells in this domain were ganglion cells and the abnormal development of this area affected the closure of the optic fissure resulting in coloboma.

View Article: PubMed Central - PubMed

Affiliation: Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany; Hartmut Hoffmann-Berling International Graduate School (HBIGS), Heidelberg, Germany.

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Quantification of cell numbers in rx2::atoh7 hatchlings. (A, B) Frontal sections of hatchling eyes, controls (A, n = 6; 6 fish) and rx2:atoh7 (n = 7; 4 fish) were used for quantification of DAPI stained nuclei within different retinal domains. Graphs are presented as mean and SEM. In the dorsal retina, GCL (green, E), INL (magenta, D) and ONL (blue, C) were analyzed. A significantly reduced number of nuclei of the INL (D) was found in rx2::atoh7 fish. In the ventral retina a separation into GCL, INL and ONL was impossible in rx2::atoh7 fish. Therefore the ventral domain was quantified in toto. A significant reduction of nuclei was found in this domain in the rx2::atoh7 fish (F).
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f0020: Quantification of cell numbers in rx2::atoh7 hatchlings. (A, B) Frontal sections of hatchling eyes, controls (A, n = 6; 6 fish) and rx2:atoh7 (n = 7; 4 fish) were used for quantification of DAPI stained nuclei within different retinal domains. Graphs are presented as mean and SEM. In the dorsal retina, GCL (green, E), INL (magenta, D) and ONL (blue, C) were analyzed. A significantly reduced number of nuclei of the INL (D) was found in rx2::atoh7 fish. In the ventral retina a separation into GCL, INL and ONL was impossible in rx2::atoh7 fish. Therefore the ventral domain was quantified in toto. A significant reduction of nuclei was found in this domain in the rx2::atoh7 fish (F).

Mentions: As described above the dorsal retinal domain appeared to be largely normal with correct layering. To further investigate a potentially subtle phenotype with respect to retinal differentiation we quantified cells in the distinct layers of the dorsal retina (GCL: ganglion cells, INL: amacrine cells, horizontal cells, bipolar cells and Müller glia cells and ONL: photoreceptor cells) of rx2::atoh7 hatchlings and controls (Fig. 4).


Differential responsiveness of distinct retinal domains to Atoh7.

Sinn R, Peravali R, Heermann S, Wittbrodt J - Mech. Dev. (2014)

Quantification of cell numbers in rx2::atoh7 hatchlings. (A, B) Frontal sections of hatchling eyes, controls (A, n = 6; 6 fish) and rx2:atoh7 (n = 7; 4 fish) were used for quantification of DAPI stained nuclei within different retinal domains. Graphs are presented as mean and SEM. In the dorsal retina, GCL (green, E), INL (magenta, D) and ONL (blue, C) were analyzed. A significantly reduced number of nuclei of the INL (D) was found in rx2::atoh7 fish. In the ventral retina a separation into GCL, INL and ONL was impossible in rx2::atoh7 fish. Therefore the ventral domain was quantified in toto. A significant reduction of nuclei was found in this domain in the rx2::atoh7 fish (F).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232737&req=5

f0020: Quantification of cell numbers in rx2::atoh7 hatchlings. (A, B) Frontal sections of hatchling eyes, controls (A, n = 6; 6 fish) and rx2:atoh7 (n = 7; 4 fish) were used for quantification of DAPI stained nuclei within different retinal domains. Graphs are presented as mean and SEM. In the dorsal retina, GCL (green, E), INL (magenta, D) and ONL (blue, C) were analyzed. A significantly reduced number of nuclei of the INL (D) was found in rx2::atoh7 fish. In the ventral retina a separation into GCL, INL and ONL was impossible in rx2::atoh7 fish. Therefore the ventral domain was quantified in toto. A significant reduction of nuclei was found in this domain in the rx2::atoh7 fish (F).
Mentions: As described above the dorsal retinal domain appeared to be largely normal with correct layering. To further investigate a potentially subtle phenotype with respect to retinal differentiation we quantified cells in the distinct layers of the dorsal retina (GCL: ganglion cells, INL: amacrine cells, horizontal cells, bipolar cells and Müller glia cells and ONL: photoreceptor cells) of rx2::atoh7 hatchlings and controls (Fig. 4).

Bottom Line: Atoh7 loss of function results in a massive reduction or even a total loss of RGCs.Photoreceptors were largely absent and the Müller glia cell number was reduced significantly.The majority of cells in this domain were ganglion cells and the abnormal development of this area affected the closure of the optic fissure resulting in coloboma.

View Article: PubMed Central - PubMed

Affiliation: Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany; Hartmut Hoffmann-Berling International Graduate School (HBIGS), Heidelberg, Germany.

Show MeSH
Related in: MedlinePlus