Limits...
Differential responsiveness of distinct retinal domains to Atoh7.

Sinn R, Peravali R, Heermann S, Wittbrodt J - Mech. Dev. (2014)

Bottom Line: Atoh7 loss of function results in a massive reduction or even a total loss of RGCs.Photoreceptors were largely absent and the Müller glia cell number was reduced significantly.The majority of cells in this domain were ganglion cells and the abnormal development of this area affected the closure of the optic fissure resulting in coloboma.

View Article: PubMed Central - PubMed

Affiliation: Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany; Hartmut Hoffmann-Berling International Graduate School (HBIGS), Heidelberg, Germany.

Show MeSH

Related in: MedlinePlus

Unaltered ventral marker expression in rx2::atoh7 embryos. (A–H) Whole mount double fluorescent (TSA, FastRed) in situ hybridizations for pax2 mRNA magenta (fast Red) and atoh7 mRNA green (TSA); DAPI nuclear stain blue; 1 μm optical section, stage 28 laterally mounted, pax2 expression domain encircled, scale bar 20 μm. (A and B) proximal optic cup of control embryo (C and D) proximal optic cup of rx2::atoh7 (E and F) distal optic cup of control embryo (G and H) distal optic cup of rx2::atoh7, note the unaltered pax2 expression domain in the ventral optic cup of rx2::atoh7 embryos.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4232737&req=5

f0060: Unaltered ventral marker expression in rx2::atoh7 embryos. (A–H) Whole mount double fluorescent (TSA, FastRed) in situ hybridizations for pax2 mRNA magenta (fast Red) and atoh7 mRNA green (TSA); DAPI nuclear stain blue; 1 μm optical section, stage 28 laterally mounted, pax2 expression domain encircled, scale bar 20 μm. (A and B) proximal optic cup of control embryo (C and D) proximal optic cup of rx2::atoh7 (E and F) distal optic cup of control embryo (G and H) distal optic cup of rx2::atoh7, note the unaltered pax2 expression domain in the ventral optic cup of rx2::atoh7 embryos.


Differential responsiveness of distinct retinal domains to Atoh7.

Sinn R, Peravali R, Heermann S, Wittbrodt J - Mech. Dev. (2014)

Unaltered ventral marker expression in rx2::atoh7 embryos. (A–H) Whole mount double fluorescent (TSA, FastRed) in situ hybridizations for pax2 mRNA magenta (fast Red) and atoh7 mRNA green (TSA); DAPI nuclear stain blue; 1 μm optical section, stage 28 laterally mounted, pax2 expression domain encircled, scale bar 20 μm. (A and B) proximal optic cup of control embryo (C and D) proximal optic cup of rx2::atoh7 (E and F) distal optic cup of control embryo (G and H) distal optic cup of rx2::atoh7, note the unaltered pax2 expression domain in the ventral optic cup of rx2::atoh7 embryos.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4232737&req=5

f0060: Unaltered ventral marker expression in rx2::atoh7 embryos. (A–H) Whole mount double fluorescent (TSA, FastRed) in situ hybridizations for pax2 mRNA magenta (fast Red) and atoh7 mRNA green (TSA); DAPI nuclear stain blue; 1 μm optical section, stage 28 laterally mounted, pax2 expression domain encircled, scale bar 20 μm. (A and B) proximal optic cup of control embryo (C and D) proximal optic cup of rx2::atoh7 (E and F) distal optic cup of control embryo (G and H) distal optic cup of rx2::atoh7, note the unaltered pax2 expression domain in the ventral optic cup of rx2::atoh7 embryos.
Bottom Line: Atoh7 loss of function results in a massive reduction or even a total loss of RGCs.Photoreceptors were largely absent and the Müller glia cell number was reduced significantly.The majority of cells in this domain were ganglion cells and the abnormal development of this area affected the closure of the optic fissure resulting in coloboma.

View Article: PubMed Central - PubMed

Affiliation: Centre for Organismal Studies, Heidelberg University, Heidelberg, Germany; Hartmut Hoffmann-Berling International Graduate School (HBIGS), Heidelberg, Germany.

Show MeSH
Related in: MedlinePlus