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Estrogenic potency of bisphenol S, polyethersulfone and their metabolites generated by the rat liver S9 fractions on a MVLN cell using a luciferase reporter gene assay.

Kang JS, Choi JS, Kim WK, Lee YJ, Park JW - Reprod. Biol. Endocrinol. (2014)

Bottom Line: BPA and BPS induced estrogenic activity whereas PES did not show any estrogenic activity in the concentrations tested.The results suggest that the metabolites of BPS and PES have estrogenic potential and the need for the assessment of both chemicals and their metabolites in other EDC evaluation studies.The estrogenic potency of PES and its metabolites is the first report in our best knowledge.

View Article: PubMed Central - PubMed

Affiliation: Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju, Gyeongnam, Republic of Korea. jwpark@kitox.re.kr.

ABSTRACT

Background: Bisphenol A (BPA) is an applied chemical that is used in many industrial fields and is a potential endocrine disruption chemical (EDC) that is found in the environment. Bisphenol S (BPS) and polyethersulfone (PES) have been suggested as putative BPA alternatives. In this study, the estrogenic potency induced by the binding of 17-beta-estradiol (E2), BPA, BPS, PES and their metabolites formed by the rat liver S9 fraction to the human estrogen receptor (ER) was estimated.

Methods: We used an in vitro bioassay based on the luciferase reporter assay in MVLN cells to evaluate the estrogenic activity of 17-beta-estradiol (E2), BPA, BPS, PES (E2: 0.001 to 0.3 nM; BPA, BPS and PES: 0.0001 to 5 microM) and their metabolites (E2: 0.05 microM; BPA, BPS and PES: 0.1 mM) according to incubation times (0, 20 and 40 min). After chemical treatment to MVLN cells for 72 hrs, and the cell viability and luciferase intensity induced were estimated, from which the estrogenic activity of the chemicals tested was evaluated.

Results: BPA and BPS induced estrogenic activity whereas PES did not show any estrogenic activity in the concentrations tested. In an in vitro assay of metabolites, BPA metabolites displayed comparable estrogenic activity with BPA and metabolites of both BPS and PES showed increasing estrogenic activity.

Conclusions: The results suggest that the metabolites of BPS and PES have estrogenic potential and the need for the assessment of both chemicals and their metabolites in other EDC evaluation studies. The estrogenic potency of PES and its metabolites is the first report in our best knowledge.

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The estrogenic activities of the metabolites of BPA, BPS and PES formed by rat liver S9 fractions according to incubation times. The BPA, BPS and PES (0.1 mM, respectively) were incubated with rat liver S9 for 0 (inactive S9), 20 and 40 min at 37°C. The difference among incubation times of each chemical was statistically analyzed by ANOVA followed by LSD test (p <0.05). All tests were performed in triplicate.
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Fig5: The estrogenic activities of the metabolites of BPA, BPS and PES formed by rat liver S9 fractions according to incubation times. The BPA, BPS and PES (0.1 mM, respectively) were incubated with rat liver S9 for 0 (inactive S9), 20 and 40 min at 37°C. The difference among incubation times of each chemical was statistically analyzed by ANOVA followed by LSD test (p <0.05). All tests were performed in triplicate.

Mentions: The estrogenic activities of E2 metabolites according to incubation times (20 and 40 min) with an active rat liver S9 fraction were 85 and 68%, respectively, when compared to that of E2 incubated at 0 min (using an inactive rat liver S9 fraction) (Figure 4). The estrogenic activities of BPA, BPS and PES metabolites also changed according to the incubation times (Figure 5). A difference in the estrogenic activity between acetonitrile-treated PES and –untreated PES was not observed (data are not shown).Figure 4


Estrogenic potency of bisphenol S, polyethersulfone and their metabolites generated by the rat liver S9 fractions on a MVLN cell using a luciferase reporter gene assay.

Kang JS, Choi JS, Kim WK, Lee YJ, Park JW - Reprod. Biol. Endocrinol. (2014)

The estrogenic activities of the metabolites of BPA, BPS and PES formed by rat liver S9 fractions according to incubation times. The BPA, BPS and PES (0.1 mM, respectively) were incubated with rat liver S9 for 0 (inactive S9), 20 and 40 min at 37°C. The difference among incubation times of each chemical was statistically analyzed by ANOVA followed by LSD test (p <0.05). All tests were performed in triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4232735&req=5

Fig5: The estrogenic activities of the metabolites of BPA, BPS and PES formed by rat liver S9 fractions according to incubation times. The BPA, BPS and PES (0.1 mM, respectively) were incubated with rat liver S9 for 0 (inactive S9), 20 and 40 min at 37°C. The difference among incubation times of each chemical was statistically analyzed by ANOVA followed by LSD test (p <0.05). All tests were performed in triplicate.
Mentions: The estrogenic activities of E2 metabolites according to incubation times (20 and 40 min) with an active rat liver S9 fraction were 85 and 68%, respectively, when compared to that of E2 incubated at 0 min (using an inactive rat liver S9 fraction) (Figure 4). The estrogenic activities of BPA, BPS and PES metabolites also changed according to the incubation times (Figure 5). A difference in the estrogenic activity between acetonitrile-treated PES and –untreated PES was not observed (data are not shown).Figure 4

Bottom Line: BPA and BPS induced estrogenic activity whereas PES did not show any estrogenic activity in the concentrations tested.The results suggest that the metabolites of BPS and PES have estrogenic potential and the need for the assessment of both chemicals and their metabolites in other EDC evaluation studies.The estrogenic potency of PES and its metabolites is the first report in our best knowledge.

View Article: PubMed Central - PubMed

Affiliation: Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju, Gyeongnam, Republic of Korea. jwpark@kitox.re.kr.

ABSTRACT

Background: Bisphenol A (BPA) is an applied chemical that is used in many industrial fields and is a potential endocrine disruption chemical (EDC) that is found in the environment. Bisphenol S (BPS) and polyethersulfone (PES) have been suggested as putative BPA alternatives. In this study, the estrogenic potency induced by the binding of 17-beta-estradiol (E2), BPA, BPS, PES and their metabolites formed by the rat liver S9 fraction to the human estrogen receptor (ER) was estimated.

Methods: We used an in vitro bioassay based on the luciferase reporter assay in MVLN cells to evaluate the estrogenic activity of 17-beta-estradiol (E2), BPA, BPS, PES (E2: 0.001 to 0.3 nM; BPA, BPS and PES: 0.0001 to 5 microM) and their metabolites (E2: 0.05 microM; BPA, BPS and PES: 0.1 mM) according to incubation times (0, 20 and 40 min). After chemical treatment to MVLN cells for 72 hrs, and the cell viability and luciferase intensity induced were estimated, from which the estrogenic activity of the chemicals tested was evaluated.

Results: BPA and BPS induced estrogenic activity whereas PES did not show any estrogenic activity in the concentrations tested. In an in vitro assay of metabolites, BPA metabolites displayed comparable estrogenic activity with BPA and metabolites of both BPS and PES showed increasing estrogenic activity.

Conclusions: The results suggest that the metabolites of BPS and PES have estrogenic potential and the need for the assessment of both chemicals and their metabolites in other EDC evaluation studies. The estrogenic potency of PES and its metabolites is the first report in our best knowledge.

Show MeSH