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Estrogenic potency of bisphenol S, polyethersulfone and their metabolites generated by the rat liver S9 fractions on a MVLN cell using a luciferase reporter gene assay.

Kang JS, Choi JS, Kim WK, Lee YJ, Park JW - Reprod. Biol. Endocrinol. (2014)

Bottom Line: BPA and BPS induced estrogenic activity whereas PES did not show any estrogenic activity in the concentrations tested.The results suggest that the metabolites of BPS and PES have estrogenic potential and the need for the assessment of both chemicals and their metabolites in other EDC evaluation studies.The estrogenic potency of PES and its metabolites is the first report in our best knowledge.

View Article: PubMed Central - PubMed

Affiliation: Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju, Gyeongnam, Republic of Korea. jwpark@kitox.re.kr.

ABSTRACT

Background: Bisphenol A (BPA) is an applied chemical that is used in many industrial fields and is a potential endocrine disruption chemical (EDC) that is found in the environment. Bisphenol S (BPS) and polyethersulfone (PES) have been suggested as putative BPA alternatives. In this study, the estrogenic potency induced by the binding of 17-beta-estradiol (E2), BPA, BPS, PES and their metabolites formed by the rat liver S9 fraction to the human estrogen receptor (ER) was estimated.

Methods: We used an in vitro bioassay based on the luciferase reporter assay in MVLN cells to evaluate the estrogenic activity of 17-beta-estradiol (E2), BPA, BPS, PES (E2: 0.001 to 0.3 nM; BPA, BPS and PES: 0.0001 to 5 microM) and their metabolites (E2: 0.05 microM; BPA, BPS and PES: 0.1 mM) according to incubation times (0, 20 and 40 min). After chemical treatment to MVLN cells for 72 hrs, and the cell viability and luciferase intensity induced were estimated, from which the estrogenic activity of the chemicals tested was evaluated.

Results: BPA and BPS induced estrogenic activity whereas PES did not show any estrogenic activity in the concentrations tested. In an in vitro assay of metabolites, BPA metabolites displayed comparable estrogenic activity with BPA and metabolites of both BPS and PES showed increasing estrogenic activity.

Conclusions: The results suggest that the metabolites of BPS and PES have estrogenic potential and the need for the assessment of both chemicals and their metabolites in other EDC evaluation studies. The estrogenic potency of PES and its metabolites is the first report in our best knowledge.

Show MeSH
The structures of BPA, BPS and PES. BPA and BPS are bisphenol analogues that have two hydroxyphenyl functionalities. PES is a polymer of BPS.
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Fig1: The structures of BPA, BPS and PES. BPA and BPS are bisphenol analogues that have two hydroxyphenyl functionalities. PES is a polymer of BPS.

Mentions: (17β)-estra-1,3,5(10)-triene-3,17-dio (17-β-estradiol, E2, CAS number: 50-28-2, ≥98%), 2,2-bis (4-hydroxyphenyl) propane (BPA, CAS number: 80-05-7, ≥99%) and 4,4'-sulfonyldiphenol (BPS, CAS number: 80-09-1, 98%) were purchased from Sigma-Aldrich (St Louis, MO, USA), and polyethersulfone (PES, CAS number: 25608-6-3, 100%) was purchased from Goodfellow Cambridge Limited (Cambridgeshire, UK). The structures of BPA, BPS and PES were shown at Figure 1. All of the test chemicals were dissolved in dimethyl sulfoxide (DMSO; Junsei Chemical, Tokyo, Japan).Figure 1


Estrogenic potency of bisphenol S, polyethersulfone and their metabolites generated by the rat liver S9 fractions on a MVLN cell using a luciferase reporter gene assay.

Kang JS, Choi JS, Kim WK, Lee YJ, Park JW - Reprod. Biol. Endocrinol. (2014)

The structures of BPA, BPS and PES. BPA and BPS are bisphenol analogues that have two hydroxyphenyl functionalities. PES is a polymer of BPS.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4232735&req=5

Fig1: The structures of BPA, BPS and PES. BPA and BPS are bisphenol analogues that have two hydroxyphenyl functionalities. PES is a polymer of BPS.
Mentions: (17β)-estra-1,3,5(10)-triene-3,17-dio (17-β-estradiol, E2, CAS number: 50-28-2, ≥98%), 2,2-bis (4-hydroxyphenyl) propane (BPA, CAS number: 80-05-7, ≥99%) and 4,4'-sulfonyldiphenol (BPS, CAS number: 80-09-1, 98%) were purchased from Sigma-Aldrich (St Louis, MO, USA), and polyethersulfone (PES, CAS number: 25608-6-3, 100%) was purchased from Goodfellow Cambridge Limited (Cambridgeshire, UK). The structures of BPA, BPS and PES were shown at Figure 1. All of the test chemicals were dissolved in dimethyl sulfoxide (DMSO; Junsei Chemical, Tokyo, Japan).Figure 1

Bottom Line: BPA and BPS induced estrogenic activity whereas PES did not show any estrogenic activity in the concentrations tested.The results suggest that the metabolites of BPS and PES have estrogenic potential and the need for the assessment of both chemicals and their metabolites in other EDC evaluation studies.The estrogenic potency of PES and its metabolites is the first report in our best knowledge.

View Article: PubMed Central - PubMed

Affiliation: Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju, Gyeongnam, Republic of Korea. jwpark@kitox.re.kr.

ABSTRACT

Background: Bisphenol A (BPA) is an applied chemical that is used in many industrial fields and is a potential endocrine disruption chemical (EDC) that is found in the environment. Bisphenol S (BPS) and polyethersulfone (PES) have been suggested as putative BPA alternatives. In this study, the estrogenic potency induced by the binding of 17-beta-estradiol (E2), BPA, BPS, PES and their metabolites formed by the rat liver S9 fraction to the human estrogen receptor (ER) was estimated.

Methods: We used an in vitro bioassay based on the luciferase reporter assay in MVLN cells to evaluate the estrogenic activity of 17-beta-estradiol (E2), BPA, BPS, PES (E2: 0.001 to 0.3 nM; BPA, BPS and PES: 0.0001 to 5 microM) and their metabolites (E2: 0.05 microM; BPA, BPS and PES: 0.1 mM) according to incubation times (0, 20 and 40 min). After chemical treatment to MVLN cells for 72 hrs, and the cell viability and luciferase intensity induced were estimated, from which the estrogenic activity of the chemicals tested was evaluated.

Results: BPA and BPS induced estrogenic activity whereas PES did not show any estrogenic activity in the concentrations tested. In an in vitro assay of metabolites, BPA metabolites displayed comparable estrogenic activity with BPA and metabolites of both BPS and PES showed increasing estrogenic activity.

Conclusions: The results suggest that the metabolites of BPS and PES have estrogenic potential and the need for the assessment of both chemicals and their metabolites in other EDC evaluation studies. The estrogenic potency of PES and its metabolites is the first report in our best knowledge.

Show MeSH